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Carboxymethyl Hyaluronan-Stabilized Nanoparticles for Anticancer Drug Delivery
Carboxymethyl hyaluronic acid (CMHA) is a semisynthetic derivative of HA that is recognized by HA binding proteins but contains an additional carboxylic acid on some of the 6-hydroxyl groups of the N-acetyl glucosamine sugar units. These studies tested the ability of CMHA to stabilize the formation...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4581577/ https://www.ncbi.nlm.nih.gov/pubmed/26448751 http://dx.doi.org/10.1155/2015/249573 |
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author | Woodman, Jessica L. Suh, Min Sung Zhang, Jianxing Kondaveeti, Yuvabharath Burgess, Diane J. White, Bruce A. Prestwich, Glenn D. Kuhn, Liisa T. |
author_facet | Woodman, Jessica L. Suh, Min Sung Zhang, Jianxing Kondaveeti, Yuvabharath Burgess, Diane J. White, Bruce A. Prestwich, Glenn D. Kuhn, Liisa T. |
author_sort | Woodman, Jessica L. |
collection | PubMed |
description | Carboxymethyl hyaluronic acid (CMHA) is a semisynthetic derivative of HA that is recognized by HA binding proteins but contains an additional carboxylic acid on some of the 6-hydroxyl groups of the N-acetyl glucosamine sugar units. These studies tested the ability of CMHA to stabilize the formation of calcium phosphate nanoparticles and evaluated their potential to target therapy resistant, CD44(+)/CD24(−/low) human breast cancer cells (BT-474(EMT)). CMHA stabilized particles (nCaP(CMHA)) were loaded with the chemotherapy drug cis-diamminedichloroplatinum(II) (CDDP) to form nCaP(CMHA)CDDP. nCaP(CMHA)CDDP was determined to be poorly crystalline hydroxyapatite, 200 nm in diameter with a −43 mV zeta potential. nCaP(CMHA)CDDP exhibited a two-day burst release of CDDP that tapered resulting in 86% release by 7 days. Surface plasmon resonance showed that nCaP(CMHA)CDDP binds to CD44, but less effectively than CMHA or hyaluronan. nCaP(CMHA-AF488) was taken up by CD44(+)/CD24(−) BT-474(EMT) breast cancer cells within 18 hours. nCaP(CMHA)CDDP was as cytotoxic as free CDDP against the BT-474(EMT) cells. Subcutaneous BT-474(EMT) tumors were more reproducibly inhibited by a near tumor dose of 2.8 mg/kg CDDP than a 7 mg/kg dose nCaP(CMHA)CDDP. This was likely due to a lack of distribution of nCaP(CMHA)CDDP throughout the dense tumor tissue that limited drug diffusion. |
format | Online Article Text |
id | pubmed-4581577 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-45815772015-10-07 Carboxymethyl Hyaluronan-Stabilized Nanoparticles for Anticancer Drug Delivery Woodman, Jessica L. Suh, Min Sung Zhang, Jianxing Kondaveeti, Yuvabharath Burgess, Diane J. White, Bruce A. Prestwich, Glenn D. Kuhn, Liisa T. Int J Cell Biol Research Article Carboxymethyl hyaluronic acid (CMHA) is a semisynthetic derivative of HA that is recognized by HA binding proteins but contains an additional carboxylic acid on some of the 6-hydroxyl groups of the N-acetyl glucosamine sugar units. These studies tested the ability of CMHA to stabilize the formation of calcium phosphate nanoparticles and evaluated their potential to target therapy resistant, CD44(+)/CD24(−/low) human breast cancer cells (BT-474(EMT)). CMHA stabilized particles (nCaP(CMHA)) were loaded with the chemotherapy drug cis-diamminedichloroplatinum(II) (CDDP) to form nCaP(CMHA)CDDP. nCaP(CMHA)CDDP was determined to be poorly crystalline hydroxyapatite, 200 nm in diameter with a −43 mV zeta potential. nCaP(CMHA)CDDP exhibited a two-day burst release of CDDP that tapered resulting in 86% release by 7 days. Surface plasmon resonance showed that nCaP(CMHA)CDDP binds to CD44, but less effectively than CMHA or hyaluronan. nCaP(CMHA-AF488) was taken up by CD44(+)/CD24(−) BT-474(EMT) breast cancer cells within 18 hours. nCaP(CMHA)CDDP was as cytotoxic as free CDDP against the BT-474(EMT) cells. Subcutaneous BT-474(EMT) tumors were more reproducibly inhibited by a near tumor dose of 2.8 mg/kg CDDP than a 7 mg/kg dose nCaP(CMHA)CDDP. This was likely due to a lack of distribution of nCaP(CMHA)CDDP throughout the dense tumor tissue that limited drug diffusion. Hindawi Publishing Corporation 2015 2015-09-10 /pmc/articles/PMC4581577/ /pubmed/26448751 http://dx.doi.org/10.1155/2015/249573 Text en Copyright © 2015 Jessica L. Woodman et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Woodman, Jessica L. Suh, Min Sung Zhang, Jianxing Kondaveeti, Yuvabharath Burgess, Diane J. White, Bruce A. Prestwich, Glenn D. Kuhn, Liisa T. Carboxymethyl Hyaluronan-Stabilized Nanoparticles for Anticancer Drug Delivery |
title | Carboxymethyl Hyaluronan-Stabilized Nanoparticles for Anticancer Drug Delivery |
title_full | Carboxymethyl Hyaluronan-Stabilized Nanoparticles for Anticancer Drug Delivery |
title_fullStr | Carboxymethyl Hyaluronan-Stabilized Nanoparticles for Anticancer Drug Delivery |
title_full_unstemmed | Carboxymethyl Hyaluronan-Stabilized Nanoparticles for Anticancer Drug Delivery |
title_short | Carboxymethyl Hyaluronan-Stabilized Nanoparticles for Anticancer Drug Delivery |
title_sort | carboxymethyl hyaluronan-stabilized nanoparticles for anticancer drug delivery |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4581577/ https://www.ncbi.nlm.nih.gov/pubmed/26448751 http://dx.doi.org/10.1155/2015/249573 |
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