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Whose Gene Is It Anyway? The Effect of Preparation Purity on Neutrophil Transcriptome Studies

Protocols for the isolation of neutrophils from whole blood often result in neutrophil preparations containing low numbers (~5%) of contaminating leukocytes, and it is possible that these contaminating cells contribute to highly sensitive assays that measure neutrophil gene expression (e.g. qPCR). W...

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Detalles Bibliográficos
Autores principales:	Thomas, Huw B., Moots, Robert J., Edwards, Steven W., Wright, Helen L.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2015
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4581699/ https://www.ncbi.nlm.nih.gov/pubmed/26401909 http://dx.doi.org/10.1371/journal.pone.0138982

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