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Anti-Arthritic Effect of Chebulanin on Collagen-Induced Arthritis in Mice

Rheumatoid arthritis is a chronic degenerative autoimmune disease characterized by persistent inflammation of synovial membranes, which leads to cartilage destruction and bone erosion. To date, there are no effective therapies to slow the progress of this degenerative condition. Here, we evaluate th...

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Detalles Bibliográficos
Autores principales: Zhao, Yinglan, Liu, Fang, Liu, Yao, Zhou, Dan, Dai, Qing, Liu, Songqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4581703/
https://www.ncbi.nlm.nih.gov/pubmed/26402786
http://dx.doi.org/10.1371/journal.pone.0139052
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author Zhao, Yinglan
Liu, Fang
Liu, Yao
Zhou, Dan
Dai, Qing
Liu, Songqing
author_facet Zhao, Yinglan
Liu, Fang
Liu, Yao
Zhou, Dan
Dai, Qing
Liu, Songqing
author_sort Zhao, Yinglan
collection PubMed
description Rheumatoid arthritis is a chronic degenerative autoimmune disease characterized by persistent inflammation of synovial membranes, which leads to cartilage destruction and bone erosion. To date, there are no effective therapies to slow the progress of this degenerative condition. Here, we evaluate the anti-arthritic effect of chebulanin, an abundant anti-inflammatory agent isolated from Terminalia chebula, in collagen induced arthritis in DBA/1 mice by intragastric administration. Arthritic severity was scored by performing histopathological evaluation of the joints and measuring the expression of inflammatory cytokines and relative enzymes by immunohistochemical staining. In parallel, bone destruction and erosion were confirmed by micro-CT. Our data revealed that chebulanin significantly improved the severity of arthritis. Specifically, the histopathological characteristics of the tissues were improved and expression of TNF-α, IL-6, MMP-3 and COX-2 in the paws and joints of the treated mice decreased in a dose-dependent manner compared with control mice. Furthermore, micro-CT analysis revealed that chebulanin induced a dose-dependent reduction in cartilage destruction and bone erosion. Taken together, our findings suggest that chebulanin suppresses the expression of inflammatory mediators and prevents cartilage destruction and bone erosion in mice. Therefore, chebulanin is a strong therapeutic alternative for the treatment of RA.
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spelling pubmed-45817032015-10-01 Anti-Arthritic Effect of Chebulanin on Collagen-Induced Arthritis in Mice Zhao, Yinglan Liu, Fang Liu, Yao Zhou, Dan Dai, Qing Liu, Songqing PLoS One Research Article Rheumatoid arthritis is a chronic degenerative autoimmune disease characterized by persistent inflammation of synovial membranes, which leads to cartilage destruction and bone erosion. To date, there are no effective therapies to slow the progress of this degenerative condition. Here, we evaluate the anti-arthritic effect of chebulanin, an abundant anti-inflammatory agent isolated from Terminalia chebula, in collagen induced arthritis in DBA/1 mice by intragastric administration. Arthritic severity was scored by performing histopathological evaluation of the joints and measuring the expression of inflammatory cytokines and relative enzymes by immunohistochemical staining. In parallel, bone destruction and erosion were confirmed by micro-CT. Our data revealed that chebulanin significantly improved the severity of arthritis. Specifically, the histopathological characteristics of the tissues were improved and expression of TNF-α, IL-6, MMP-3 and COX-2 in the paws and joints of the treated mice decreased in a dose-dependent manner compared with control mice. Furthermore, micro-CT analysis revealed that chebulanin induced a dose-dependent reduction in cartilage destruction and bone erosion. Taken together, our findings suggest that chebulanin suppresses the expression of inflammatory mediators and prevents cartilage destruction and bone erosion in mice. Therefore, chebulanin is a strong therapeutic alternative for the treatment of RA. Public Library of Science 2015-09-24 /pmc/articles/PMC4581703/ /pubmed/26402786 http://dx.doi.org/10.1371/journal.pone.0139052 Text en © 2015 Zhao et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Zhao, Yinglan
Liu, Fang
Liu, Yao
Zhou, Dan
Dai, Qing
Liu, Songqing
Anti-Arthritic Effect of Chebulanin on Collagen-Induced Arthritis in Mice
title Anti-Arthritic Effect of Chebulanin on Collagen-Induced Arthritis in Mice
title_full Anti-Arthritic Effect of Chebulanin on Collagen-Induced Arthritis in Mice
title_fullStr Anti-Arthritic Effect of Chebulanin on Collagen-Induced Arthritis in Mice
title_full_unstemmed Anti-Arthritic Effect of Chebulanin on Collagen-Induced Arthritis in Mice
title_short Anti-Arthritic Effect of Chebulanin on Collagen-Induced Arthritis in Mice
title_sort anti-arthritic effect of chebulanin on collagen-induced arthritis in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4581703/
https://www.ncbi.nlm.nih.gov/pubmed/26402786
http://dx.doi.org/10.1371/journal.pone.0139052
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