Cargando…
The Role of Dietary Extra Virgin Olive Oil and Corn Oil on the Alteration of Epigenetic Patterns in the Rat DMBA-Induced Breast Cancer Model
Disruption of epigenetic patterns is a major change occurring in all types of cancers. Such alterations are characterized by global DNA hypomethylation, gene-promoter hypermethylation and aberrant histone modifications, and may be modified by environment. Nutritional factors, and especially dietary...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4581736/ https://www.ncbi.nlm.nih.gov/pubmed/26401660 http://dx.doi.org/10.1371/journal.pone.0138980 |
_version_ | 1782391608794152960 |
---|---|
author | Rodríguez-Miguel, Cristina Moral, Raquel Escrich, Raquel Vela, Elena Solanas, Montserrat Escrich, Eduard |
author_facet | Rodríguez-Miguel, Cristina Moral, Raquel Escrich, Raquel Vela, Elena Solanas, Montserrat Escrich, Eduard |
author_sort | Rodríguez-Miguel, Cristina |
collection | PubMed |
description | Disruption of epigenetic patterns is a major change occurring in all types of cancers. Such alterations are characterized by global DNA hypomethylation, gene-promoter hypermethylation and aberrant histone modifications, and may be modified by environment. Nutritional factors, and especially dietary lipids, have a role in the etiology of breast cancer. Thus, we aimed to analyze the influence of different high fat diets on DNA methylation and histone modifications in the rat dimethylbenz(a)anthracene (DMBA)-induced breast cancer model. Female Sprague-Dawley rats were fed a low-fat, a high corn-oil or a high extra-virgin olive oil (EVOO) diet from weaning or from induction with DMBA. In mammary glands and tumors we analyzed global and gene specific (RASSF1A, TIMP3) DNA methylation by LUMA and bisulfite pyrosequencing assays, respectively. We also determined gene expression and enzymatic activity of DNA methyltransferases (DNMT1, DNMT3a and DNMT3b) and evaluated changes in histone modifications (H3K4me2, H3K27me3, H4K20me3 and H4K16ac) by western-blot. Our results showed variations along time in the global DNA methylation of the mammary gland displaying decreases at puberty and with aging. The olive oil-enriched diet, on the one hand, increased the levels of global DNA methylation in mammary gland and tumor, and on the other, changed histone modifications patterns. The corn oil-enriched diet increased DNA methyltransferase activity in both tissues, resulting in an increase in the promoter methylation of the tumor suppressor genes RASSF1A and TIMP3. These results suggest a differential effect of the high fat diets on epigenetic patterns with a relevant role in the neoplastic transformation, which could be one of the mechanisms of their differential promoter effect, clearly stimulating for the high corn-oil diet and with a weaker influence for the high EVOO diet, on breast cancer progression. |
format | Online Article Text |
id | pubmed-4581736 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-45817362015-10-01 The Role of Dietary Extra Virgin Olive Oil and Corn Oil on the Alteration of Epigenetic Patterns in the Rat DMBA-Induced Breast Cancer Model Rodríguez-Miguel, Cristina Moral, Raquel Escrich, Raquel Vela, Elena Solanas, Montserrat Escrich, Eduard PLoS One Research Article Disruption of epigenetic patterns is a major change occurring in all types of cancers. Such alterations are characterized by global DNA hypomethylation, gene-promoter hypermethylation and aberrant histone modifications, and may be modified by environment. Nutritional factors, and especially dietary lipids, have a role in the etiology of breast cancer. Thus, we aimed to analyze the influence of different high fat diets on DNA methylation and histone modifications in the rat dimethylbenz(a)anthracene (DMBA)-induced breast cancer model. Female Sprague-Dawley rats were fed a low-fat, a high corn-oil or a high extra-virgin olive oil (EVOO) diet from weaning or from induction with DMBA. In mammary glands and tumors we analyzed global and gene specific (RASSF1A, TIMP3) DNA methylation by LUMA and bisulfite pyrosequencing assays, respectively. We also determined gene expression and enzymatic activity of DNA methyltransferases (DNMT1, DNMT3a and DNMT3b) and evaluated changes in histone modifications (H3K4me2, H3K27me3, H4K20me3 and H4K16ac) by western-blot. Our results showed variations along time in the global DNA methylation of the mammary gland displaying decreases at puberty and with aging. The olive oil-enriched diet, on the one hand, increased the levels of global DNA methylation in mammary gland and tumor, and on the other, changed histone modifications patterns. The corn oil-enriched diet increased DNA methyltransferase activity in both tissues, resulting in an increase in the promoter methylation of the tumor suppressor genes RASSF1A and TIMP3. These results suggest a differential effect of the high fat diets on epigenetic patterns with a relevant role in the neoplastic transformation, which could be one of the mechanisms of their differential promoter effect, clearly stimulating for the high corn-oil diet and with a weaker influence for the high EVOO diet, on breast cancer progression. Public Library of Science 2015-09-24 /pmc/articles/PMC4581736/ /pubmed/26401660 http://dx.doi.org/10.1371/journal.pone.0138980 Text en © 2015 Rodríguez-Miguel et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Rodríguez-Miguel, Cristina Moral, Raquel Escrich, Raquel Vela, Elena Solanas, Montserrat Escrich, Eduard The Role of Dietary Extra Virgin Olive Oil and Corn Oil on the Alteration of Epigenetic Patterns in the Rat DMBA-Induced Breast Cancer Model |
title | The Role of Dietary Extra Virgin Olive Oil and Corn Oil on the Alteration of Epigenetic Patterns in the Rat DMBA-Induced Breast Cancer Model |
title_full | The Role of Dietary Extra Virgin Olive Oil and Corn Oil on the Alteration of Epigenetic Patterns in the Rat DMBA-Induced Breast Cancer Model |
title_fullStr | The Role of Dietary Extra Virgin Olive Oil and Corn Oil on the Alteration of Epigenetic Patterns in the Rat DMBA-Induced Breast Cancer Model |
title_full_unstemmed | The Role of Dietary Extra Virgin Olive Oil and Corn Oil on the Alteration of Epigenetic Patterns in the Rat DMBA-Induced Breast Cancer Model |
title_short | The Role of Dietary Extra Virgin Olive Oil and Corn Oil on the Alteration of Epigenetic Patterns in the Rat DMBA-Induced Breast Cancer Model |
title_sort | role of dietary extra virgin olive oil and corn oil on the alteration of epigenetic patterns in the rat dmba-induced breast cancer model |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4581736/ https://www.ncbi.nlm.nih.gov/pubmed/26401660 http://dx.doi.org/10.1371/journal.pone.0138980 |
work_keys_str_mv | AT rodriguezmiguelcristina theroleofdietaryextravirginoliveoilandcornoilonthealterationofepigeneticpatternsintheratdmbainducedbreastcancermodel AT moralraquel theroleofdietaryextravirginoliveoilandcornoilonthealterationofepigeneticpatternsintheratdmbainducedbreastcancermodel AT escrichraquel theroleofdietaryextravirginoliveoilandcornoilonthealterationofepigeneticpatternsintheratdmbainducedbreastcancermodel AT velaelena theroleofdietaryextravirginoliveoilandcornoilonthealterationofepigeneticpatternsintheratdmbainducedbreastcancermodel AT solanasmontserrat theroleofdietaryextravirginoliveoilandcornoilonthealterationofepigeneticpatternsintheratdmbainducedbreastcancermodel AT escricheduard theroleofdietaryextravirginoliveoilandcornoilonthealterationofepigeneticpatternsintheratdmbainducedbreastcancermodel AT rodriguezmiguelcristina roleofdietaryextravirginoliveoilandcornoilonthealterationofepigeneticpatternsintheratdmbainducedbreastcancermodel AT moralraquel roleofdietaryextravirginoliveoilandcornoilonthealterationofepigeneticpatternsintheratdmbainducedbreastcancermodel AT escrichraquel roleofdietaryextravirginoliveoilandcornoilonthealterationofepigeneticpatternsintheratdmbainducedbreastcancermodel AT velaelena roleofdietaryextravirginoliveoilandcornoilonthealterationofepigeneticpatternsintheratdmbainducedbreastcancermodel AT solanasmontserrat roleofdietaryextravirginoliveoilandcornoilonthealterationofepigeneticpatternsintheratdmbainducedbreastcancermodel AT escricheduard roleofdietaryextravirginoliveoilandcornoilonthealterationofepigeneticpatternsintheratdmbainducedbreastcancermodel |