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Protective effects of SS31 on t-BHP induced oxidative damage in 661W cells

The present study aimed to investigate the ability of SS31, a novel mitochondria-targeted peptide to protect against t-BHP-induced mitochondrial dysfunction and apoptosis in 661W cell lines. The 661W cells were treated with various concentrations of SS-31 and an MTT assay was used to determine cell...

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Autores principales: MA, WEI, ZHU, XIAOBO, DING, XIAOYAN, LI, TAO, HU, YIJUN, HU, XUTING, YUAN, LIN, LEI, LEI, HU, ANDINA, LUO, YAN, TANG, SHIBO
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4581771/
https://www.ncbi.nlm.nih.gov/pubmed/26165373
http://dx.doi.org/10.3892/mmr.2015.4055
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author MA, WEI
ZHU, XIAOBO
DING, XIAOYAN
LI, TAO
HU, YIJUN
HU, XUTING
YUAN, LIN
LEI, LEI
HU, ANDINA
LUO, YAN
TANG, SHIBO
author_facet MA, WEI
ZHU, XIAOBO
DING, XIAOYAN
LI, TAO
HU, YIJUN
HU, XUTING
YUAN, LIN
LEI, LEI
HU, ANDINA
LUO, YAN
TANG, SHIBO
author_sort MA, WEI
collection PubMed
description The present study aimed to investigate the ability of SS31, a novel mitochondria-targeted peptide to protect against t-BHP-induced mitochondrial dysfunction and apoptosis in 661W cell lines. The 661W cells were treated with various concentrations of SS-31 and an MTT assay was used to determine cell viability. The expression of nitrotyrosine and 8-hydroxydeoxyguanosine (8-OHdG) was detected using immunofluorescent staining. Apoptosis were assessed using Hoechst staining and an annexin V/propidium iodide flow cytometer. Reactive oxygen species (ROS) were detected using MitoSOX(™) with confocal microscopy. Changes in mitochondrial membrane potential were analyzed using flow cytometry. In addition, the release of cytochrome c was analyzed using confocal microscopy. The viability of the cells improved following treatment with SS31 between 100 nM and 1 µM, compared with untreated control group. Compared with the t-BHP treatment group (20.0±3.8%), the number of annexin V-positive cells decreased dose-dependently to 13.6±2.6, 9.8±0.5 and 7.4±2.0% in the SS-31 treated group at concentrations of 10 nM, 100 nM and 1 µM, respectively. Treatment with SS-31 significantly prevented the t-BHP-induced expression of nitrotyrosine and 8-OHdG, decreased the quantity of mitochondrial ROS, increased mitochondrial potential, and prevented the release of cytochrome c from mitochondria into the cytoplasm. Therefore, the SS31 mitochondria-targeted peptide protected the 661W cells from the sustained oxidative stress induced by t-BHP.
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spelling pubmed-45817712015-11-30 Protective effects of SS31 on t-BHP induced oxidative damage in 661W cells MA, WEI ZHU, XIAOBO DING, XIAOYAN LI, TAO HU, YIJUN HU, XUTING YUAN, LIN LEI, LEI HU, ANDINA LUO, YAN TANG, SHIBO Mol Med Rep Articles The present study aimed to investigate the ability of SS31, a novel mitochondria-targeted peptide to protect against t-BHP-induced mitochondrial dysfunction and apoptosis in 661W cell lines. The 661W cells were treated with various concentrations of SS-31 and an MTT assay was used to determine cell viability. The expression of nitrotyrosine and 8-hydroxydeoxyguanosine (8-OHdG) was detected using immunofluorescent staining. Apoptosis were assessed using Hoechst staining and an annexin V/propidium iodide flow cytometer. Reactive oxygen species (ROS) were detected using MitoSOX(™) with confocal microscopy. Changes in mitochondrial membrane potential were analyzed using flow cytometry. In addition, the release of cytochrome c was analyzed using confocal microscopy. The viability of the cells improved following treatment with SS31 between 100 nM and 1 µM, compared with untreated control group. Compared with the t-BHP treatment group (20.0±3.8%), the number of annexin V-positive cells decreased dose-dependently to 13.6±2.6, 9.8±0.5 and 7.4±2.0% in the SS-31 treated group at concentrations of 10 nM, 100 nM and 1 µM, respectively. Treatment with SS-31 significantly prevented the t-BHP-induced expression of nitrotyrosine and 8-OHdG, decreased the quantity of mitochondrial ROS, increased mitochondrial potential, and prevented the release of cytochrome c from mitochondria into the cytoplasm. Therefore, the SS31 mitochondria-targeted peptide protected the 661W cells from the sustained oxidative stress induced by t-BHP. D.A. Spandidos 2015-10 2015-07-07 /pmc/articles/PMC4581771/ /pubmed/26165373 http://dx.doi.org/10.3892/mmr.2015.4055 Text en Copyright: © Ma. https://creativecommons.org/licenses/by-nc-nd/4.0 This is an open access article distributed under the terms of a Creative Commons Attribution License
spellingShingle Articles
MA, WEI
ZHU, XIAOBO
DING, XIAOYAN
LI, TAO
HU, YIJUN
HU, XUTING
YUAN, LIN
LEI, LEI
HU, ANDINA
LUO, YAN
TANG, SHIBO
Protective effects of SS31 on t-BHP induced oxidative damage in 661W cells
title Protective effects of SS31 on t-BHP induced oxidative damage in 661W cells
title_full Protective effects of SS31 on t-BHP induced oxidative damage in 661W cells
title_fullStr Protective effects of SS31 on t-BHP induced oxidative damage in 661W cells
title_full_unstemmed Protective effects of SS31 on t-BHP induced oxidative damage in 661W cells
title_short Protective effects of SS31 on t-BHP induced oxidative damage in 661W cells
title_sort protective effects of ss31 on t-bhp induced oxidative damage in 661w cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4581771/
https://www.ncbi.nlm.nih.gov/pubmed/26165373
http://dx.doi.org/10.3892/mmr.2015.4055
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