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Ponicidin suppresses HT29 cell growth via the induction of G1 cell cycle arrest and apoptosis

Ponicidin is a diterpenoid extracted from the Chinese herb Isodon adenolomus, which has been reported as a therapeutic cytotoxic drug that may be used to treat various types of human cancer. The present study aimed to determine the antitumor effects of ponicidin, and to investigate its underlying me...

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Autores principales: DU, JIE, CHEN, CHUNYOU, SUN, YIQUN, ZHENG, LIN, WANG, WANCHEN
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4581821/
https://www.ncbi.nlm.nih.gov/pubmed/26239027
http://dx.doi.org/10.3892/mmr.2015.4150
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author DU, JIE
CHEN, CHUNYOU
SUN, YIQUN
ZHENG, LIN
WANG, WANCHEN
author_facet DU, JIE
CHEN, CHUNYOU
SUN, YIQUN
ZHENG, LIN
WANG, WANCHEN
author_sort DU, JIE
collection PubMed
description Ponicidin is a diterpenoid extracted from the Chinese herb Isodon adenolomus, which has been reported as a therapeutic cytotoxic drug that may be used to treat various types of human cancer. The present study aimed to determine the antitumor effects of ponicidin, and to investigate its underlying mechanisms in colorectal cancer. The HT29 colorectal cancer cell line was used to detect the cytotoxicity of various doses of ponicidin. Cell proliferation was measured using a Cell Counting kit-8 assay. Cell cycle and apoptosis analyses were performed using flow cytometry and fluorescent microscopy. Western blot analysis was used to measure the expression levels of apoptosis-associated proteins following treatment with ponicidin. Treatment with ponicidin significantly suppressed HT29 cell growth by inducing G1 cell cycle arrest and apoptosis. The AKT and MEK signaling pathways were also suppressed by ponicidin; however, the p38 signaling pathway was significantly activated. The expression levels of caspase 3 and Bax protein were markedly upregulated following treatment with ponicidin. These results suggest that ponicidin exerts significant antitumor effects via the induction of cell cycle arrest and apoptosis in colorectal cells. In conclusion, ponicidin acted as an inducer of apoptosis, and may be used as a therapeutic cytotoxic drug to treat human cancer, including colorectal cancer.
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spelling pubmed-45818212015-11-30 Ponicidin suppresses HT29 cell growth via the induction of G1 cell cycle arrest and apoptosis DU, JIE CHEN, CHUNYOU SUN, YIQUN ZHENG, LIN WANG, WANCHEN Mol Med Rep Articles Ponicidin is a diterpenoid extracted from the Chinese herb Isodon adenolomus, which has been reported as a therapeutic cytotoxic drug that may be used to treat various types of human cancer. The present study aimed to determine the antitumor effects of ponicidin, and to investigate its underlying mechanisms in colorectal cancer. The HT29 colorectal cancer cell line was used to detect the cytotoxicity of various doses of ponicidin. Cell proliferation was measured using a Cell Counting kit-8 assay. Cell cycle and apoptosis analyses were performed using flow cytometry and fluorescent microscopy. Western blot analysis was used to measure the expression levels of apoptosis-associated proteins following treatment with ponicidin. Treatment with ponicidin significantly suppressed HT29 cell growth by inducing G1 cell cycle arrest and apoptosis. The AKT and MEK signaling pathways were also suppressed by ponicidin; however, the p38 signaling pathway was significantly activated. The expression levels of caspase 3 and Bax protein were markedly upregulated following treatment with ponicidin. These results suggest that ponicidin exerts significant antitumor effects via the induction of cell cycle arrest and apoptosis in colorectal cells. In conclusion, ponicidin acted as an inducer of apoptosis, and may be used as a therapeutic cytotoxic drug to treat human cancer, including colorectal cancer. D.A. Spandidos 2015-10 2015-07-29 /pmc/articles/PMC4581821/ /pubmed/26239027 http://dx.doi.org/10.3892/mmr.2015.4150 Text en Copyright: © Du. https://creativecommons.org/licenses/by-nc-nd/4.0 This is an open access article distributed under the terms of a Creative Commons Attribution License
spellingShingle Articles
DU, JIE
CHEN, CHUNYOU
SUN, YIQUN
ZHENG, LIN
WANG, WANCHEN
Ponicidin suppresses HT29 cell growth via the induction of G1 cell cycle arrest and apoptosis
title Ponicidin suppresses HT29 cell growth via the induction of G1 cell cycle arrest and apoptosis
title_full Ponicidin suppresses HT29 cell growth via the induction of G1 cell cycle arrest and apoptosis
title_fullStr Ponicidin suppresses HT29 cell growth via the induction of G1 cell cycle arrest and apoptosis
title_full_unstemmed Ponicidin suppresses HT29 cell growth via the induction of G1 cell cycle arrest and apoptosis
title_short Ponicidin suppresses HT29 cell growth via the induction of G1 cell cycle arrest and apoptosis
title_sort ponicidin suppresses ht29 cell growth via the induction of g1 cell cycle arrest and apoptosis
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4581821/
https://www.ncbi.nlm.nih.gov/pubmed/26239027
http://dx.doi.org/10.3892/mmr.2015.4150
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