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Flagellar membranes are rich in raft-forming phospholipids
The observation that the membranes of flagella are enriched in sterols and sphingolipids has led to the hypothesis that flagella might be enriched in raft-forming lipids. However, a detailed lipidomic analysis of flagellar membranes is not available. Novel protocols to detach and isolate intact flag...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4582118/ https://www.ncbi.nlm.nih.gov/pubmed/26276100 http://dx.doi.org/10.1242/bio.011957 |
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author | Serricchio, Mauro Schmid, Adrien W. Steinmann, Michael E. Sigel, Erwin Rauch, Monika Julkowska, Daria Bonnefoy, Serge Fort, Cécile Bastin, Philippe Bütikofer, Peter |
author_facet | Serricchio, Mauro Schmid, Adrien W. Steinmann, Michael E. Sigel, Erwin Rauch, Monika Julkowska, Daria Bonnefoy, Serge Fort, Cécile Bastin, Philippe Bütikofer, Peter |
author_sort | Serricchio, Mauro |
collection | PubMed |
description | The observation that the membranes of flagella are enriched in sterols and sphingolipids has led to the hypothesis that flagella might be enriched in raft-forming lipids. However, a detailed lipidomic analysis of flagellar membranes is not available. Novel protocols to detach and isolate intact flagella from Trypanosoma brucei procyclic forms in combination with reverse-phase liquid chromatography high-resolution tandem mass spectrometry allowed us to determine the phospholipid composition of flagellar membranes relative to whole cells. Our analyses revealed that phosphatidylethanolamine, phosphatidylserine, ceramide and the sphingolipids inositol phosphorylceramide and sphingomyelin are enriched in flagella relative to whole cells. In contrast, phosphatidylcholine and phosphatidylinositol are strongly depleted in flagella. Within individual glycerophospholipid classes, we observed a preference for ether-type over diacyl-type molecular species in membranes of flagella. Our study provides direct evidence for a preferential presence of raft-forming phospholipids in flagellar membranes of T. brucei. |
format | Online Article Text |
id | pubmed-4582118 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | The Company of Biologists |
record_format | MEDLINE/PubMed |
spelling | pubmed-45821182015-10-02 Flagellar membranes are rich in raft-forming phospholipids Serricchio, Mauro Schmid, Adrien W. Steinmann, Michael E. Sigel, Erwin Rauch, Monika Julkowska, Daria Bonnefoy, Serge Fort, Cécile Bastin, Philippe Bütikofer, Peter Biol Open Research Article The observation that the membranes of flagella are enriched in sterols and sphingolipids has led to the hypothesis that flagella might be enriched in raft-forming lipids. However, a detailed lipidomic analysis of flagellar membranes is not available. Novel protocols to detach and isolate intact flagella from Trypanosoma brucei procyclic forms in combination with reverse-phase liquid chromatography high-resolution tandem mass spectrometry allowed us to determine the phospholipid composition of flagellar membranes relative to whole cells. Our analyses revealed that phosphatidylethanolamine, phosphatidylserine, ceramide and the sphingolipids inositol phosphorylceramide and sphingomyelin are enriched in flagella relative to whole cells. In contrast, phosphatidylcholine and phosphatidylinositol are strongly depleted in flagella. Within individual glycerophospholipid classes, we observed a preference for ether-type over diacyl-type molecular species in membranes of flagella. Our study provides direct evidence for a preferential presence of raft-forming phospholipids in flagellar membranes of T. brucei. The Company of Biologists 2015-08-14 /pmc/articles/PMC4582118/ /pubmed/26276100 http://dx.doi.org/10.1242/bio.011957 Text en © 2015. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Research Article Serricchio, Mauro Schmid, Adrien W. Steinmann, Michael E. Sigel, Erwin Rauch, Monika Julkowska, Daria Bonnefoy, Serge Fort, Cécile Bastin, Philippe Bütikofer, Peter Flagellar membranes are rich in raft-forming phospholipids |
title | Flagellar membranes are rich in raft-forming phospholipids |
title_full | Flagellar membranes are rich in raft-forming phospholipids |
title_fullStr | Flagellar membranes are rich in raft-forming phospholipids |
title_full_unstemmed | Flagellar membranes are rich in raft-forming phospholipids |
title_short | Flagellar membranes are rich in raft-forming phospholipids |
title_sort | flagellar membranes are rich in raft-forming phospholipids |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4582118/ https://www.ncbi.nlm.nih.gov/pubmed/26276100 http://dx.doi.org/10.1242/bio.011957 |
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