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Differentiation of adipose-derived stem cells into Schwann-like cells: fetal bovine serum or human serum?

Access to autologous Schwann cells is limited due to lack of donor site and its difficult isolation and culture. Therefore, one of the possible ways to obtain to Schwann cells is to differentiate mesenchymal stem cells into glial pathway using various materials and protocols. The aim of this study w...

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Autores principales: Younesi, Elham, Bayati, Vahid, Hashemitabar, Mahmoud, Azandeh, Seyyed Saeed, Bijannejad, Dariush, Bahreini, Amin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Association of Anatomists 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4582159/
https://www.ncbi.nlm.nih.gov/pubmed/26417476
http://dx.doi.org/10.5115/acb.2015.48.3.170
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author Younesi, Elham
Bayati, Vahid
Hashemitabar, Mahmoud
Azandeh, Seyyed Saeed
Bijannejad, Dariush
Bahreini, Amin
author_facet Younesi, Elham
Bayati, Vahid
Hashemitabar, Mahmoud
Azandeh, Seyyed Saeed
Bijannejad, Dariush
Bahreini, Amin
author_sort Younesi, Elham
collection PubMed
description Access to autologous Schwann cells is limited due to lack of donor site and its difficult isolation and culture. Therefore, one of the possible ways to obtain to Schwann cells is to differentiate mesenchymal stem cells into glial pathway using various materials and protocols. The aim of this study was to compare the effects of fetal bovine serum and human serum on Schwann cell differentiation of adipose-derived stem cells to choose the best serum for use in future research. For this purpose, after isolation of human adipose-derived stem cells, it was characterized and differentiated into Schwann cell lineage using two protocols which one of them contained fetal bovine serum and the other human serum. At the end, morphological evaluation declared an increased detachment of cells in response to human serum. On the other side, immunocytochemistry showed that there was a significant increase in the number of cells expressing glial fibrillary acidic proteins and S100 in fetal bovine serum-treated group when compared to human serum-treated one (P<0.05). It was concluded that fetal bovine serum was more effective than allogeneic human serum in Schwann cell differentiation of adipose-derived stem cells.
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spelling pubmed-45821592015-09-28 Differentiation of adipose-derived stem cells into Schwann-like cells: fetal bovine serum or human serum? Younesi, Elham Bayati, Vahid Hashemitabar, Mahmoud Azandeh, Seyyed Saeed Bijannejad, Dariush Bahreini, Amin Anat Cell Biol Original Article Access to autologous Schwann cells is limited due to lack of donor site and its difficult isolation and culture. Therefore, one of the possible ways to obtain to Schwann cells is to differentiate mesenchymal stem cells into glial pathway using various materials and protocols. The aim of this study was to compare the effects of fetal bovine serum and human serum on Schwann cell differentiation of adipose-derived stem cells to choose the best serum for use in future research. For this purpose, after isolation of human adipose-derived stem cells, it was characterized and differentiated into Schwann cell lineage using two protocols which one of them contained fetal bovine serum and the other human serum. At the end, morphological evaluation declared an increased detachment of cells in response to human serum. On the other side, immunocytochemistry showed that there was a significant increase in the number of cells expressing glial fibrillary acidic proteins and S100 in fetal bovine serum-treated group when compared to human serum-treated one (P<0.05). It was concluded that fetal bovine serum was more effective than allogeneic human serum in Schwann cell differentiation of adipose-derived stem cells. Korean Association of Anatomists 2015-09 2015-09-22 /pmc/articles/PMC4582159/ /pubmed/26417476 http://dx.doi.org/10.5115/acb.2015.48.3.170 Text en Copyright © 2015. Anatomy & Cell Biology http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Younesi, Elham
Bayati, Vahid
Hashemitabar, Mahmoud
Azandeh, Seyyed Saeed
Bijannejad, Dariush
Bahreini, Amin
Differentiation of adipose-derived stem cells into Schwann-like cells: fetal bovine serum or human serum?
title Differentiation of adipose-derived stem cells into Schwann-like cells: fetal bovine serum or human serum?
title_full Differentiation of adipose-derived stem cells into Schwann-like cells: fetal bovine serum or human serum?
title_fullStr Differentiation of adipose-derived stem cells into Schwann-like cells: fetal bovine serum or human serum?
title_full_unstemmed Differentiation of adipose-derived stem cells into Schwann-like cells: fetal bovine serum or human serum?
title_short Differentiation of adipose-derived stem cells into Schwann-like cells: fetal bovine serum or human serum?
title_sort differentiation of adipose-derived stem cells into schwann-like cells: fetal bovine serum or human serum?
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4582159/
https://www.ncbi.nlm.nih.gov/pubmed/26417476
http://dx.doi.org/10.5115/acb.2015.48.3.170
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