Cargando…

Human epidermal neural crest stem cells as a source of Schwann cells

We show that highly pure populations of human Schwann cells can be derived rapidly and in a straightforward way, without the need for genetic manipulation, from human epidermal neural crest stem cells [hEPI-NCSC(s)] present in the bulge of hair follicles. These human Schwann cells promise to be a us...

Descripción completa

Detalles Bibliográficos
Autores principales: Sakaue, Motoharu, Sieber-Blum, Maya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4582175/
https://www.ncbi.nlm.nih.gov/pubmed/26251357
http://dx.doi.org/10.1242/dev.123034
_version_ 1782391660225757184
author Sakaue, Motoharu
Sieber-Blum, Maya
author_facet Sakaue, Motoharu
Sieber-Blum, Maya
author_sort Sakaue, Motoharu
collection PubMed
description We show that highly pure populations of human Schwann cells can be derived rapidly and in a straightforward way, without the need for genetic manipulation, from human epidermal neural crest stem cells [hEPI-NCSC(s)] present in the bulge of hair follicles. These human Schwann cells promise to be a useful tool for cell-based therapies, disease modelling and drug discovery. Schwann cells are glia that support axons of peripheral nerves and are direct descendants of the embryonic neural crest. Peripheral nerves are damaged in various conditions, including through trauma or tumour-related surgery, and Schwann cells are required for their repair and regeneration. Schwann cells also promise to be useful for treating spinal cord injuries. Ex vivo expansion of hEPI-NCSC isolated from hair bulge explants, manipulating the WNT, sonic hedgehog and TGFβ signalling pathways, and exposure of the cells to pertinent growth factors led to the expression of the Schwann cell markers SOX10, KROX20 (EGR2), p75NTR (NGFR), MBP and S100B by day 4 in virtually all cells, and maturation was completed by 2 weeks of differentiation. Gene expression profiling demonstrated expression of transcripts for neurotrophic and angiogenic factors, as well as JUN, all of which are essential for nerve regeneration. Co-culture of hEPI-NCSC-derived human Schwann cells with rodent dorsal root ganglia showed interaction of the Schwann cells with axons, providing evidence of Schwann cell functionality. We conclude that hEPI-NCSCs are a biologically relevant source for generating large and highly pure populations of human Schwann cells.
format Online
Article
Text
id pubmed-4582175
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher The Company of Biologists
record_format MEDLINE/PubMed
spelling pubmed-45821752015-11-04 Human epidermal neural crest stem cells as a source of Schwann cells Sakaue, Motoharu Sieber-Blum, Maya Development Stem Cells and Regeneration We show that highly pure populations of human Schwann cells can be derived rapidly and in a straightforward way, without the need for genetic manipulation, from human epidermal neural crest stem cells [hEPI-NCSC(s)] present in the bulge of hair follicles. These human Schwann cells promise to be a useful tool for cell-based therapies, disease modelling and drug discovery. Schwann cells are glia that support axons of peripheral nerves and are direct descendants of the embryonic neural crest. Peripheral nerves are damaged in various conditions, including through trauma or tumour-related surgery, and Schwann cells are required for their repair and regeneration. Schwann cells also promise to be useful for treating spinal cord injuries. Ex vivo expansion of hEPI-NCSC isolated from hair bulge explants, manipulating the WNT, sonic hedgehog and TGFβ signalling pathways, and exposure of the cells to pertinent growth factors led to the expression of the Schwann cell markers SOX10, KROX20 (EGR2), p75NTR (NGFR), MBP and S100B by day 4 in virtually all cells, and maturation was completed by 2 weeks of differentiation. Gene expression profiling demonstrated expression of transcripts for neurotrophic and angiogenic factors, as well as JUN, all of which are essential for nerve regeneration. Co-culture of hEPI-NCSC-derived human Schwann cells with rodent dorsal root ganglia showed interaction of the Schwann cells with axons, providing evidence of Schwann cell functionality. We conclude that hEPI-NCSCs are a biologically relevant source for generating large and highly pure populations of human Schwann cells. The Company of Biologists 2015-09-15 /pmc/articles/PMC4582175/ /pubmed/26251357 http://dx.doi.org/10.1242/dev.123034 Text en © 2015. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Stem Cells and Regeneration
Sakaue, Motoharu
Sieber-Blum, Maya
Human epidermal neural crest stem cells as a source of Schwann cells
title Human epidermal neural crest stem cells as a source of Schwann cells
title_full Human epidermal neural crest stem cells as a source of Schwann cells
title_fullStr Human epidermal neural crest stem cells as a source of Schwann cells
title_full_unstemmed Human epidermal neural crest stem cells as a source of Schwann cells
title_short Human epidermal neural crest stem cells as a source of Schwann cells
title_sort human epidermal neural crest stem cells as a source of schwann cells
topic Stem Cells and Regeneration
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4582175/
https://www.ncbi.nlm.nih.gov/pubmed/26251357
http://dx.doi.org/10.1242/dev.123034
work_keys_str_mv AT sakauemotoharu humanepidermalneuralcreststemcellsasasourceofschwanncells
AT sieberblummaya humanepidermalneuralcreststemcellsasasourceofschwanncells