Determination of the infectious titer and virulence of an original US porcine epidemic diarrhea virus PC22A strain

The infectious dose of a virus pool of original US PEDV strain PC22A was determined in 4-day-old, cesarean-derived, colostrum-deprived (CDCD) piglets. The median pig diarrhea dose (PDD(50)) of the virus pool was determined as 7.35 log(10) PDD(50)/mL, similar to the cell culture infectious titer, 7.7...

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Autores principales: Liu, Xinsheng, Lin, Chun-Ming, Annamalai, Thavamathi, Gao, Xiang, Lu, Zhongyan, Esseili, Malak A, Jung, Kwonil, El-Tholoth, Mohamed, Saif, Linda J, Wang, Qiuhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4582625/
https://www.ncbi.nlm.nih.gov/pubmed/26408019
http://dx.doi.org/10.1186/s13567-015-0249-1
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author Liu, Xinsheng
Lin, Chun-Ming
Annamalai, Thavamathi
Gao, Xiang
Lu, Zhongyan
Esseili, Malak A
Jung, Kwonil
El-Tholoth, Mohamed
Saif, Linda J
Wang, Qiuhong
author_facet Liu, Xinsheng
Lin, Chun-Ming
Annamalai, Thavamathi
Gao, Xiang
Lu, Zhongyan
Esseili, Malak A
Jung, Kwonil
El-Tholoth, Mohamed
Saif, Linda J
Wang, Qiuhong
author_sort Liu, Xinsheng
collection PubMed
description The infectious dose of a virus pool of original US PEDV strain PC22A was determined in 4-day-old, cesarean-derived, colostrum-deprived (CDCD) piglets. The median pig diarrhea dose (PDD(50)) of the virus pool was determined as 7.35 log(10) PDD(50)/mL, similar to the cell culture infectious titer, 7.75 log(10) plaque-forming units (PFU)/mL. 100 PDD(50) caused watery diarrhea in all conventional suckling piglets (n = 12) derived from a PEDV-naive sow, whereas 1000 and 10 000 PDD(50) did not cause diarrhea in piglets derived from two PEDV-field exposed-recovered sows. This information is important for future PEDV challenge studies and validation of PEDV vaccines.
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spelling pubmed-45826252015-09-26 Determination of the infectious titer and virulence of an original US porcine epidemic diarrhea virus PC22A strain Liu, Xinsheng Lin, Chun-Ming Annamalai, Thavamathi Gao, Xiang Lu, Zhongyan Esseili, Malak A Jung, Kwonil El-Tholoth, Mohamed Saif, Linda J Wang, Qiuhong Vet Res Short Report The infectious dose of a virus pool of original US PEDV strain PC22A was determined in 4-day-old, cesarean-derived, colostrum-deprived (CDCD) piglets. The median pig diarrhea dose (PDD(50)) of the virus pool was determined as 7.35 log(10) PDD(50)/mL, similar to the cell culture infectious titer, 7.75 log(10) plaque-forming units (PFU)/mL. 100 PDD(50) caused watery diarrhea in all conventional suckling piglets (n = 12) derived from a PEDV-naive sow, whereas 1000 and 10 000 PDD(50) did not cause diarrhea in piglets derived from two PEDV-field exposed-recovered sows. This information is important for future PEDV challenge studies and validation of PEDV vaccines. BioMed Central 2015-09-25 2015 /pmc/articles/PMC4582625/ /pubmed/26408019 http://dx.doi.org/10.1186/s13567-015-0249-1 Text en © Liu et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Report
Liu, Xinsheng
Lin, Chun-Ming
Annamalai, Thavamathi
Gao, Xiang
Lu, Zhongyan
Esseili, Malak A
Jung, Kwonil
El-Tholoth, Mohamed
Saif, Linda J
Wang, Qiuhong
Determination of the infectious titer and virulence of an original US porcine epidemic diarrhea virus PC22A strain
title Determination of the infectious titer and virulence of an original US porcine epidemic diarrhea virus PC22A strain
title_full Determination of the infectious titer and virulence of an original US porcine epidemic diarrhea virus PC22A strain
title_fullStr Determination of the infectious titer and virulence of an original US porcine epidemic diarrhea virus PC22A strain
title_full_unstemmed Determination of the infectious titer and virulence of an original US porcine epidemic diarrhea virus PC22A strain
title_short Determination of the infectious titer and virulence of an original US porcine epidemic diarrhea virus PC22A strain
title_sort determination of the infectious titer and virulence of an original us porcine epidemic diarrhea virus pc22a strain
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4582625/
https://www.ncbi.nlm.nih.gov/pubmed/26408019
http://dx.doi.org/10.1186/s13567-015-0249-1
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