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Experimental infection of cattle with ovine Dichelobacter nodosus isolates

BACKGROUND: Dichelobacter nodosus is the main causative agent of ovine footrot, and there are strong indications that the bacterium can be transferred to cattle grazing on the same pasture as sheep. The aim of this study was to investigate if benign and virulent D. nodosus strains isolated from shee...

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Autores principales: Knappe-Poindecker, Maren, Jørgensen, Hannah Joan, Jensen, Tim Kåre, Tesfamichael, Bereket, Ulvund, Martha Jakobsen, Hektoen, Lisbeth, Fjeldaas, Terje
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4582627/
https://www.ncbi.nlm.nih.gov/pubmed/26407552
http://dx.doi.org/10.1186/s13028-015-0148-5
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author Knappe-Poindecker, Maren
Jørgensen, Hannah Joan
Jensen, Tim Kåre
Tesfamichael, Bereket
Ulvund, Martha Jakobsen
Hektoen, Lisbeth
Fjeldaas, Terje
author_facet Knappe-Poindecker, Maren
Jørgensen, Hannah Joan
Jensen, Tim Kåre
Tesfamichael, Bereket
Ulvund, Martha Jakobsen
Hektoen, Lisbeth
Fjeldaas, Terje
author_sort Knappe-Poindecker, Maren
collection PubMed
description BACKGROUND: Dichelobacter nodosus is the main causative agent of ovine footrot, and there are strong indications that the bacterium can be transferred to cattle grazing on the same pasture as sheep. The aim of this study was to investigate if benign and virulent D. nodosus strains isolated from sheep can be transferred to the interdigital skin of cattle under experimental conditions. Further, we wanted to observe the impact of such infection on bovine foot health, and test the effect of topical chlortetracycline (Cyclo spray(®): Eurovet) on the infection. FINDINGS: Six heifers were included in the study. After an initial 18-day maceration period, three heifers were inoculated on one single foot with a benign strain and three with a virulent strain by adding bacterial suspension in a bandage. The bandages were left on for 17 days, and when removed, D. nodosus was isolated from all six heifers. All six heifers developed interdigital dermatitis. In five of the heifers D. nodosus organisms were demonstrated within the epidermis. Twenty-four days after treatment with chlortetracycline all heifers were negative by cultivation, but tested positive for D. nodosus by polymerase chain reaction (PCR). Two of the six heifers still tested positive for D. nodosus by PCR 49 days after treatment. After 70 days, all heifers tested negative for D. nodosus. CONCLUSIONS: This study shows that both virulent and benign D. nodosus strains originating from sheep can be transferred to naïve heifers under experimental conditions. Further, the study supports the hypothesis that infections with virulent D. nodosus in cattle are associated with interdigital dermatitis. No conclusion regarding the treatment of D. nodosus infection with chlortetracycline was possible.
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spelling pubmed-45826272015-09-26 Experimental infection of cattle with ovine Dichelobacter nodosus isolates Knappe-Poindecker, Maren Jørgensen, Hannah Joan Jensen, Tim Kåre Tesfamichael, Bereket Ulvund, Martha Jakobsen Hektoen, Lisbeth Fjeldaas, Terje Acta Vet Scand Brief Communication BACKGROUND: Dichelobacter nodosus is the main causative agent of ovine footrot, and there are strong indications that the bacterium can be transferred to cattle grazing on the same pasture as sheep. The aim of this study was to investigate if benign and virulent D. nodosus strains isolated from sheep can be transferred to the interdigital skin of cattle under experimental conditions. Further, we wanted to observe the impact of such infection on bovine foot health, and test the effect of topical chlortetracycline (Cyclo spray(®): Eurovet) on the infection. FINDINGS: Six heifers were included in the study. After an initial 18-day maceration period, three heifers were inoculated on one single foot with a benign strain and three with a virulent strain by adding bacterial suspension in a bandage. The bandages were left on for 17 days, and when removed, D. nodosus was isolated from all six heifers. All six heifers developed interdigital dermatitis. In five of the heifers D. nodosus organisms were demonstrated within the epidermis. Twenty-four days after treatment with chlortetracycline all heifers were negative by cultivation, but tested positive for D. nodosus by polymerase chain reaction (PCR). Two of the six heifers still tested positive for D. nodosus by PCR 49 days after treatment. After 70 days, all heifers tested negative for D. nodosus. CONCLUSIONS: This study shows that both virulent and benign D. nodosus strains originating from sheep can be transferred to naïve heifers under experimental conditions. Further, the study supports the hypothesis that infections with virulent D. nodosus in cattle are associated with interdigital dermatitis. No conclusion regarding the treatment of D. nodosus infection with chlortetracycline was possible. BioMed Central 2015-09-25 /pmc/articles/PMC4582627/ /pubmed/26407552 http://dx.doi.org/10.1186/s13028-015-0148-5 Text en © Knappe-Poindecker et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Brief Communication
Knappe-Poindecker, Maren
Jørgensen, Hannah Joan
Jensen, Tim Kåre
Tesfamichael, Bereket
Ulvund, Martha Jakobsen
Hektoen, Lisbeth
Fjeldaas, Terje
Experimental infection of cattle with ovine Dichelobacter nodosus isolates
title Experimental infection of cattle with ovine Dichelobacter nodosus isolates
title_full Experimental infection of cattle with ovine Dichelobacter nodosus isolates
title_fullStr Experimental infection of cattle with ovine Dichelobacter nodosus isolates
title_full_unstemmed Experimental infection of cattle with ovine Dichelobacter nodosus isolates
title_short Experimental infection of cattle with ovine Dichelobacter nodosus isolates
title_sort experimental infection of cattle with ovine dichelobacter nodosus isolates
topic Brief Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4582627/
https://www.ncbi.nlm.nih.gov/pubmed/26407552
http://dx.doi.org/10.1186/s13028-015-0148-5
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