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The complete genome sequence of the rumen methanogen Methanosarcina barkeri CM1

Methanosarcina species are the most metabolically versatile of the methanogenic Archaea and can obtain energy for growth by producing methane via the hydrogenotrophic, acetoclastic or methylotrophic pathways. Methanosarcina barkeri CM1 was isolated from the rumen of a New Zealand Friesian cow grazin...

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Autores principales: Lambie, Suzanne C., Kelly, William J., Leahy, Sinead C., Li, Dong, Reilly, Kerri, McAllister, Tim A., Valle, Edith R., Attwood, Graeme T., Altermann, Eric
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4582637/
https://www.ncbi.nlm.nih.gov/pubmed/26413197
http://dx.doi.org/10.1186/s40793-015-0038-5
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author Lambie, Suzanne C.
Kelly, William J.
Leahy, Sinead C.
Li, Dong
Reilly, Kerri
McAllister, Tim A.
Valle, Edith R.
Attwood, Graeme T.
Altermann, Eric
author_facet Lambie, Suzanne C.
Kelly, William J.
Leahy, Sinead C.
Li, Dong
Reilly, Kerri
McAllister, Tim A.
Valle, Edith R.
Attwood, Graeme T.
Altermann, Eric
author_sort Lambie, Suzanne C.
collection PubMed
description Methanosarcina species are the most metabolically versatile of the methanogenic Archaea and can obtain energy for growth by producing methane via the hydrogenotrophic, acetoclastic or methylotrophic pathways. Methanosarcina barkeri CM1 was isolated from the rumen of a New Zealand Friesian cow grazing a ryegrass/clover pasture, and its genome has been sequenced to provide information on the phylogenetic diversity of rumen methanogens with a view to developing technologies for methane mitigation. The 4.5 Mb chromosome has an average G + C content of 39 %, and encodes 3523 protein-coding genes, but has no plasmid or prophage sequences. The gene content is very similar to that of M. barkeri Fusaro which was isolated from freshwater sediment. CM1 has a full complement of genes for all three methanogenesis pathways, but its genome shows many differences from those of other sequenced rumen methanogens. Consequently strategies to mitigate ruminant methane need to include information on the different methanogens that occur in the rumen. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s40793-015-0038-5) contains supplementary material, which is available to authorized users.
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spelling pubmed-45826372015-09-26 The complete genome sequence of the rumen methanogen Methanosarcina barkeri CM1 Lambie, Suzanne C. Kelly, William J. Leahy, Sinead C. Li, Dong Reilly, Kerri McAllister, Tim A. Valle, Edith R. Attwood, Graeme T. Altermann, Eric Stand Genomic Sci Short Genome Report Methanosarcina species are the most metabolically versatile of the methanogenic Archaea and can obtain energy for growth by producing methane via the hydrogenotrophic, acetoclastic or methylotrophic pathways. Methanosarcina barkeri CM1 was isolated from the rumen of a New Zealand Friesian cow grazing a ryegrass/clover pasture, and its genome has been sequenced to provide information on the phylogenetic diversity of rumen methanogens with a view to developing technologies for methane mitigation. The 4.5 Mb chromosome has an average G + C content of 39 %, and encodes 3523 protein-coding genes, but has no plasmid or prophage sequences. The gene content is very similar to that of M. barkeri Fusaro which was isolated from freshwater sediment. CM1 has a full complement of genes for all three methanogenesis pathways, but its genome shows many differences from those of other sequenced rumen methanogens. Consequently strategies to mitigate ruminant methane need to include information on the different methanogens that occur in the rumen. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s40793-015-0038-5) contains supplementary material, which is available to authorized users. BioMed Central 2015-08-19 /pmc/articles/PMC4582637/ /pubmed/26413197 http://dx.doi.org/10.1186/s40793-015-0038-5 Text en © Lambie et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Genome Report
Lambie, Suzanne C.
Kelly, William J.
Leahy, Sinead C.
Li, Dong
Reilly, Kerri
McAllister, Tim A.
Valle, Edith R.
Attwood, Graeme T.
Altermann, Eric
The complete genome sequence of the rumen methanogen Methanosarcina barkeri CM1
title The complete genome sequence of the rumen methanogen Methanosarcina barkeri CM1
title_full The complete genome sequence of the rumen methanogen Methanosarcina barkeri CM1
title_fullStr The complete genome sequence of the rumen methanogen Methanosarcina barkeri CM1
title_full_unstemmed The complete genome sequence of the rumen methanogen Methanosarcina barkeri CM1
title_short The complete genome sequence of the rumen methanogen Methanosarcina barkeri CM1
title_sort complete genome sequence of the rumen methanogen methanosarcina barkeri cm1
topic Short Genome Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4582637/
https://www.ncbi.nlm.nih.gov/pubmed/26413197
http://dx.doi.org/10.1186/s40793-015-0038-5
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