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Intracellular microlasers
Optical microresonators(1) which confine light within a small cavity are widely exploited for various applications ranging from the realization of lasers(2) and nonlinear devices(3, 4, 5) to biochemical and optomechanical sensing(6, 7, 8, 9, 10, 11). Here we employ microresonators and suitable optic...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2015
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4583142/ https://www.ncbi.nlm.nih.gov/pubmed/26417383 http://dx.doi.org/10.1038/nphoton.2015.129 |
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author | Humar, Matjaž Yun, Seok Hyun |
author_facet | Humar, Matjaž Yun, Seok Hyun |
author_sort | Humar, Matjaž |
collection | PubMed |
description | Optical microresonators(1) which confine light within a small cavity are widely exploited for various applications ranging from the realization of lasers(2) and nonlinear devices(3, 4, 5) to biochemical and optomechanical sensing(6, 7, 8, 9, 10, 11). Here we employ microresonators and suitable optical gain materials inside biological cells to demonstrate various optical functions in vitro including lasing. We explored two distinct types of microresonators: soft and hard, that support whispering-gallery modes (WGM). Soft droplets formed by injecting oil or using natural lipid droplets support intracellular laser action. The laser spectra from oil-droplet microlasers can chart cytoplasmic internal stress (~500 pN/μm(2)) and its dynamic fluctuations at a sensitivity of 20 pN/μm(2) (20 Pa). In a second form, WGMs within phagocytized polystyrene beads of different sizes enable individual tagging of thousands of cells easily and, in principle, a much larger number by multiplexing with different dyes. |
format | Online Article Text |
id | pubmed-4583142 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
record_format | MEDLINE/PubMed |
spelling | pubmed-45831422016-03-01 Intracellular microlasers Humar, Matjaž Yun, Seok Hyun Nat Photonics Article Optical microresonators(1) which confine light within a small cavity are widely exploited for various applications ranging from the realization of lasers(2) and nonlinear devices(3, 4, 5) to biochemical and optomechanical sensing(6, 7, 8, 9, 10, 11). Here we employ microresonators and suitable optical gain materials inside biological cells to demonstrate various optical functions in vitro including lasing. We explored two distinct types of microresonators: soft and hard, that support whispering-gallery modes (WGM). Soft droplets formed by injecting oil or using natural lipid droplets support intracellular laser action. The laser spectra from oil-droplet microlasers can chart cytoplasmic internal stress (~500 pN/μm(2)) and its dynamic fluctuations at a sensitivity of 20 pN/μm(2) (20 Pa). In a second form, WGMs within phagocytized polystyrene beads of different sizes enable individual tagging of thousands of cells easily and, in principle, a much larger number by multiplexing with different dyes. 2015-07-25 2015-09-01 /pmc/articles/PMC4583142/ /pubmed/26417383 http://dx.doi.org/10.1038/nphoton.2015.129 Text en Reprints and permissions information is available online at www.nature.com/reprints (http://www.nature.com/reprints) . |
spellingShingle | Article Humar, Matjaž Yun, Seok Hyun Intracellular microlasers |
title | Intracellular microlasers |
title_full | Intracellular microlasers |
title_fullStr | Intracellular microlasers |
title_full_unstemmed | Intracellular microlasers |
title_short | Intracellular microlasers |
title_sort | intracellular microlasers |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4583142/ https://www.ncbi.nlm.nih.gov/pubmed/26417383 http://dx.doi.org/10.1038/nphoton.2015.129 |
work_keys_str_mv | AT humarmatjaz intracellularmicrolasers AT yunseokhyun intracellularmicrolasers |