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Intracellular microlasers

Optical microresonators(1) which confine light within a small cavity are widely exploited for various applications ranging from the realization of lasers(2) and nonlinear devices(3, 4, 5) to biochemical and optomechanical sensing(6, 7, 8, 9, 10, 11). Here we employ microresonators and suitable optic...

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Detalles Bibliográficos
Autores principales: Humar, Matjaž, Yun, Seok Hyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4583142/
https://www.ncbi.nlm.nih.gov/pubmed/26417383
http://dx.doi.org/10.1038/nphoton.2015.129
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author Humar, Matjaž
Yun, Seok Hyun
author_facet Humar, Matjaž
Yun, Seok Hyun
author_sort Humar, Matjaž
collection PubMed
description Optical microresonators(1) which confine light within a small cavity are widely exploited for various applications ranging from the realization of lasers(2) and nonlinear devices(3, 4, 5) to biochemical and optomechanical sensing(6, 7, 8, 9, 10, 11). Here we employ microresonators and suitable optical gain materials inside biological cells to demonstrate various optical functions in vitro including lasing. We explored two distinct types of microresonators: soft and hard, that support whispering-gallery modes (WGM). Soft droplets formed by injecting oil or using natural lipid droplets support intracellular laser action. The laser spectra from oil-droplet microlasers can chart cytoplasmic internal stress (~500 pN/μm(2)) and its dynamic fluctuations at a sensitivity of 20 pN/μm(2) (20 Pa). In a second form, WGMs within phagocytized polystyrene beads of different sizes enable individual tagging of thousands of cells easily and, in principle, a much larger number by multiplexing with different dyes.
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spelling pubmed-45831422016-03-01 Intracellular microlasers Humar, Matjaž Yun, Seok Hyun Nat Photonics Article Optical microresonators(1) which confine light within a small cavity are widely exploited for various applications ranging from the realization of lasers(2) and nonlinear devices(3, 4, 5) to biochemical and optomechanical sensing(6, 7, 8, 9, 10, 11). Here we employ microresonators and suitable optical gain materials inside biological cells to demonstrate various optical functions in vitro including lasing. We explored two distinct types of microresonators: soft and hard, that support whispering-gallery modes (WGM). Soft droplets formed by injecting oil or using natural lipid droplets support intracellular laser action. The laser spectra from oil-droplet microlasers can chart cytoplasmic internal stress (~500 pN/μm(2)) and its dynamic fluctuations at a sensitivity of 20 pN/μm(2) (20 Pa). In a second form, WGMs within phagocytized polystyrene beads of different sizes enable individual tagging of thousands of cells easily and, in principle, a much larger number by multiplexing with different dyes. 2015-07-25 2015-09-01 /pmc/articles/PMC4583142/ /pubmed/26417383 http://dx.doi.org/10.1038/nphoton.2015.129 Text en Reprints and permissions information is available online at www.nature.com/reprints (http://www.nature.com/reprints) .
spellingShingle Article
Humar, Matjaž
Yun, Seok Hyun
Intracellular microlasers
title Intracellular microlasers
title_full Intracellular microlasers
title_fullStr Intracellular microlasers
title_full_unstemmed Intracellular microlasers
title_short Intracellular microlasers
title_sort intracellular microlasers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4583142/
https://www.ncbi.nlm.nih.gov/pubmed/26417383
http://dx.doi.org/10.1038/nphoton.2015.129
work_keys_str_mv AT humarmatjaz intracellularmicrolasers
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