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Differential Expression of Ecdysone Receptor Leads to Variation in Phenotypic Plasticity across Serial Homologs

Bodies are often made of repeated units, or serial homologs, that develop using the same core gene regulatory network. Local inputs and modifications to this network allow serial homologs to evolve different morphologies, but currently we do not understand which modifications allow these repeated tr...

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Autores principales: Monteiro, Antónia, Tong, Xiaoling, Bear, Ashley, Liew, Seng Fatt, Bhardwaj, Shivam, Wasik, Bethany R., Dinwiddie, April, Bastianelli, Carole, Cheong, Wei Fun, Wenk, Markus R., Cao, Hui, Prudic, Kathleen L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4583414/
https://www.ncbi.nlm.nih.gov/pubmed/26405828
http://dx.doi.org/10.1371/journal.pgen.1005529
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author Monteiro, Antónia
Tong, Xiaoling
Bear, Ashley
Liew, Seng Fatt
Bhardwaj, Shivam
Wasik, Bethany R.
Dinwiddie, April
Bastianelli, Carole
Cheong, Wei Fun
Wenk, Markus R.
Cao, Hui
Prudic, Kathleen L.
author_facet Monteiro, Antónia
Tong, Xiaoling
Bear, Ashley
Liew, Seng Fatt
Bhardwaj, Shivam
Wasik, Bethany R.
Dinwiddie, April
Bastianelli, Carole
Cheong, Wei Fun
Wenk, Markus R.
Cao, Hui
Prudic, Kathleen L.
author_sort Monteiro, Antónia
collection PubMed
description Bodies are often made of repeated units, or serial homologs, that develop using the same core gene regulatory network. Local inputs and modifications to this network allow serial homologs to evolve different morphologies, but currently we do not understand which modifications allow these repeated traits to evolve different levels of phenotypic plasticity. Here we describe variation in phenotypic plasticity across serial homologous eyespots of the butterfly Bicyclus anynana, hypothesized to be under selection for similar or different functions in the wet and dry seasonal forms. Specifically, we document the presence of eyespot size and scale brightness plasticity in hindwing eyespots hypothesized to vary in function across seasons, and reduced size plasticity and absence of brightness plasticity in forewing eyespots hypothesized to have the same function across seasons. By exploring the molecular and physiological causes of this variation in plasticity across fore and hindwing serial homologs we discover that: 1) temperature experienced during the wandering stages of larval development alters titers of an ecdysteroid hormone, 20-hydroxyecdysone (20E), in the hemolymph of wet and dry seasonal forms at that stage; 2) the 20E receptor (EcR) is differentially expressed in the forewing and hindwing eyespot centers of both seasonal forms during this critical developmental stage; and 3) manipulations of EcR signaling disproportionately affected hindwing eyespots relative to forewing eyespots. We propose that differential EcR expression across forewing and hindwing eyespots at a critical stage of development explains the variation in levels of phenotypic plasticity across these serial homologues. This finding provides a novel signaling pathway, 20E, and a novel molecular candidate, EcR, for the regulation of levels of phenotypic plasticity across body parts or serial homologs.
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spelling pubmed-45834142015-10-02 Differential Expression of Ecdysone Receptor Leads to Variation in Phenotypic Plasticity across Serial Homologs Monteiro, Antónia Tong, Xiaoling Bear, Ashley Liew, Seng Fatt Bhardwaj, Shivam Wasik, Bethany R. Dinwiddie, April Bastianelli, Carole Cheong, Wei Fun Wenk, Markus R. Cao, Hui Prudic, Kathleen L. PLoS Genet Research Article Bodies are often made of repeated units, or serial homologs, that develop using the same core gene regulatory network. Local inputs and modifications to this network allow serial homologs to evolve different morphologies, but currently we do not understand which modifications allow these repeated traits to evolve different levels of phenotypic plasticity. Here we describe variation in phenotypic plasticity across serial homologous eyespots of the butterfly Bicyclus anynana, hypothesized to be under selection for similar or different functions in the wet and dry seasonal forms. Specifically, we document the presence of eyespot size and scale brightness plasticity in hindwing eyespots hypothesized to vary in function across seasons, and reduced size plasticity and absence of brightness plasticity in forewing eyespots hypothesized to have the same function across seasons. By exploring the molecular and physiological causes of this variation in plasticity across fore and hindwing serial homologs we discover that: 1) temperature experienced during the wandering stages of larval development alters titers of an ecdysteroid hormone, 20-hydroxyecdysone (20E), in the hemolymph of wet and dry seasonal forms at that stage; 2) the 20E receptor (EcR) is differentially expressed in the forewing and hindwing eyespot centers of both seasonal forms during this critical developmental stage; and 3) manipulations of EcR signaling disproportionately affected hindwing eyespots relative to forewing eyespots. We propose that differential EcR expression across forewing and hindwing eyespots at a critical stage of development explains the variation in levels of phenotypic plasticity across these serial homologues. This finding provides a novel signaling pathway, 20E, and a novel molecular candidate, EcR, for the regulation of levels of phenotypic plasticity across body parts or serial homologs. Public Library of Science 2015-09-25 /pmc/articles/PMC4583414/ /pubmed/26405828 http://dx.doi.org/10.1371/journal.pgen.1005529 Text en © 2015 Monteiro et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Monteiro, Antónia
Tong, Xiaoling
Bear, Ashley
Liew, Seng Fatt
Bhardwaj, Shivam
Wasik, Bethany R.
Dinwiddie, April
Bastianelli, Carole
Cheong, Wei Fun
Wenk, Markus R.
Cao, Hui
Prudic, Kathleen L.
Differential Expression of Ecdysone Receptor Leads to Variation in Phenotypic Plasticity across Serial Homologs
title Differential Expression of Ecdysone Receptor Leads to Variation in Phenotypic Plasticity across Serial Homologs
title_full Differential Expression of Ecdysone Receptor Leads to Variation in Phenotypic Plasticity across Serial Homologs
title_fullStr Differential Expression of Ecdysone Receptor Leads to Variation in Phenotypic Plasticity across Serial Homologs
title_full_unstemmed Differential Expression of Ecdysone Receptor Leads to Variation in Phenotypic Plasticity across Serial Homologs
title_short Differential Expression of Ecdysone Receptor Leads to Variation in Phenotypic Plasticity across Serial Homologs
title_sort differential expression of ecdysone receptor leads to variation in phenotypic plasticity across serial homologs
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4583414/
https://www.ncbi.nlm.nih.gov/pubmed/26405828
http://dx.doi.org/10.1371/journal.pgen.1005529
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