Cargando…

Diversity in boron toxicity tolerance of Australian barley (Hordeum vulgare L.) genotypes

BACKGROUND: Boron (B) is an important micronutrient for plant growth, but is toxic when levels are too high. This commonly occurs in environments with alkaline soils and relatively low rainfall, including many of the cereal growing regions of southern Australia. Four major genetic loci controlling t...

Descripción completa

Detalles Bibliográficos
Autores principales: Hayes, Julie E., Pallotta, Margaret, Garcia, Melissa, Öz, Mehmet Tufan, Rongala, Jay, Sutton, Tim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4584011/
https://www.ncbi.nlm.nih.gov/pubmed/26410221
http://dx.doi.org/10.1186/s12870-015-0607-1
_version_ 1782391921960812544
author Hayes, Julie E.
Pallotta, Margaret
Garcia, Melissa
Öz, Mehmet Tufan
Rongala, Jay
Sutton, Tim
author_facet Hayes, Julie E.
Pallotta, Margaret
Garcia, Melissa
Öz, Mehmet Tufan
Rongala, Jay
Sutton, Tim
author_sort Hayes, Julie E.
collection PubMed
description BACKGROUND: Boron (B) is an important micronutrient for plant growth, but is toxic when levels are too high. This commonly occurs in environments with alkaline soils and relatively low rainfall, including many of the cereal growing regions of southern Australia. Four major genetic loci controlling tolerance to high soil B have been identified in the landrace barley, Sahara 3771. Genes underlying two of the loci encode the B transporters HvBot1 and HvNIP2;1. RESULTS: We investigated sequence and expression level diversity in HvBot1 and HvNIP2;1 across barley germplasm, and identified five novel coding sequence alleles for HvBot1. Lines were identified containing either single or multiple copies of the Sahara HvBot1 allele. We established that only the tandemly duplicated Sahara allele conferred B tolerance, and this duplicated allele was found only in a set of nine lines accessioned in Australian collections as Sahara 3763–3771. HvNIP2;1 coding sequences were highly conserved across barley germplasm. We identified the likely causative SNP in the 5’UTR of Sahara HvNIP2;1, and propose that the creation of a small upstream open reading frame interferes with HvNIP2;1 translation in Sahara 3771. Similar to HvBot1, the tolerant HvNIP2;1 allele was unique to the Sahara barley accessions. We identified a new source of the 2H B tolerance allele controlling leaf symptom development, in the landrace Ethiopia 756. CONCLUSIONS: Ethiopia 756, as well as the cultivar Sloop Vic which carries both the 2H and HvBot1 B tolerance alleles derived from Sahara 3771, may be valuable as alternative parents in breeding programs targeted to high soil B environments. There is significant diversity in B toxicity tolerance among contemporary Australian barley varieties but this is not related to variation at any of the four known B tolerance loci, indicating that novel, as yet undiscovered, sources of tolerance exist. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12870-015-0607-1) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-4584011
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-45840112015-09-28 Diversity in boron toxicity tolerance of Australian barley (Hordeum vulgare L.) genotypes Hayes, Julie E. Pallotta, Margaret Garcia, Melissa Öz, Mehmet Tufan Rongala, Jay Sutton, Tim BMC Plant Biol Research Article BACKGROUND: Boron (B) is an important micronutrient for plant growth, but is toxic when levels are too high. This commonly occurs in environments with alkaline soils and relatively low rainfall, including many of the cereal growing regions of southern Australia. Four major genetic loci controlling tolerance to high soil B have been identified in the landrace barley, Sahara 3771. Genes underlying two of the loci encode the B transporters HvBot1 and HvNIP2;1. RESULTS: We investigated sequence and expression level diversity in HvBot1 and HvNIP2;1 across barley germplasm, and identified five novel coding sequence alleles for HvBot1. Lines were identified containing either single or multiple copies of the Sahara HvBot1 allele. We established that only the tandemly duplicated Sahara allele conferred B tolerance, and this duplicated allele was found only in a set of nine lines accessioned in Australian collections as Sahara 3763–3771. HvNIP2;1 coding sequences were highly conserved across barley germplasm. We identified the likely causative SNP in the 5’UTR of Sahara HvNIP2;1, and propose that the creation of a small upstream open reading frame interferes with HvNIP2;1 translation in Sahara 3771. Similar to HvBot1, the tolerant HvNIP2;1 allele was unique to the Sahara barley accessions. We identified a new source of the 2H B tolerance allele controlling leaf symptom development, in the landrace Ethiopia 756. CONCLUSIONS: Ethiopia 756, as well as the cultivar Sloop Vic which carries both the 2H and HvBot1 B tolerance alleles derived from Sahara 3771, may be valuable as alternative parents in breeding programs targeted to high soil B environments. There is significant diversity in B toxicity tolerance among contemporary Australian barley varieties but this is not related to variation at any of the four known B tolerance loci, indicating that novel, as yet undiscovered, sources of tolerance exist. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12870-015-0607-1) contains supplementary material, which is available to authorized users. BioMed Central 2015-09-26 /pmc/articles/PMC4584011/ /pubmed/26410221 http://dx.doi.org/10.1186/s12870-015-0607-1 Text en © Hayes et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Hayes, Julie E.
Pallotta, Margaret
Garcia, Melissa
Öz, Mehmet Tufan
Rongala, Jay
Sutton, Tim
Diversity in boron toxicity tolerance of Australian barley (Hordeum vulgare L.) genotypes
title Diversity in boron toxicity tolerance of Australian barley (Hordeum vulgare L.) genotypes
title_full Diversity in boron toxicity tolerance of Australian barley (Hordeum vulgare L.) genotypes
title_fullStr Diversity in boron toxicity tolerance of Australian barley (Hordeum vulgare L.) genotypes
title_full_unstemmed Diversity in boron toxicity tolerance of Australian barley (Hordeum vulgare L.) genotypes
title_short Diversity in boron toxicity tolerance of Australian barley (Hordeum vulgare L.) genotypes
title_sort diversity in boron toxicity tolerance of australian barley (hordeum vulgare l.) genotypes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4584011/
https://www.ncbi.nlm.nih.gov/pubmed/26410221
http://dx.doi.org/10.1186/s12870-015-0607-1
work_keys_str_mv AT hayesjuliee diversityinborontoxicitytoleranceofaustralianbarleyhordeumvulgarelgenotypes
AT pallottamargaret diversityinborontoxicitytoleranceofaustralianbarleyhordeumvulgarelgenotypes
AT garciamelissa diversityinborontoxicitytoleranceofaustralianbarleyhordeumvulgarelgenotypes
AT ozmehmettufan diversityinborontoxicitytoleranceofaustralianbarleyhordeumvulgarelgenotypes
AT rongalajay diversityinborontoxicitytoleranceofaustralianbarleyhordeumvulgarelgenotypes
AT suttontim diversityinborontoxicitytoleranceofaustralianbarleyhordeumvulgarelgenotypes