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Rapid detection of Acinetobacter baumannii and molecular epidemiology of carbapenem-resistant A. baumannii in two comprehensive hospitals of Beijing, China

Acinetobacter baumannii is an important opportunistic pathogen associated with a variety of nosocomial infections. A rapid and sensitive molecular detection in clinical isolates is quite needed for the appropriate therapy and outbreak control of A. baumannii. Group 2 carbapenems have been considered...

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Autores principales: Li, Puyuan, Niu, Wenkai, Li, Huan, Lei, Hong, Liu, Wei, Zhao, Xiangna, Guo, Leijing, Zou, Dayang, Yuan, Xin, Liu, Huiying, Yuan, Jing, Bai, Changqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4585070/
https://www.ncbi.nlm.nih.gov/pubmed/26441924
http://dx.doi.org/10.3389/fmicb.2015.00997
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author Li, Puyuan
Niu, Wenkai
Li, Huan
Lei, Hong
Liu, Wei
Zhao, Xiangna
Guo, Leijing
Zou, Dayang
Yuan, Xin
Liu, Huiying
Yuan, Jing
Bai, Changqing
author_facet Li, Puyuan
Niu, Wenkai
Li, Huan
Lei, Hong
Liu, Wei
Zhao, Xiangna
Guo, Leijing
Zou, Dayang
Yuan, Xin
Liu, Huiying
Yuan, Jing
Bai, Changqing
author_sort Li, Puyuan
collection PubMed
description Acinetobacter baumannii is an important opportunistic pathogen associated with a variety of nosocomial infections. A rapid and sensitive molecular detection in clinical isolates is quite needed for the appropriate therapy and outbreak control of A. baumannii. Group 2 carbapenems have been considered the agents of choice for the treatment of multiple drug-resistant A. baumannii. But the prevalence of carbapenem-resistant A. baumannii (CRAB) has been steadily increasing in recent years. Here, we developed a loop-mediated isothermal amplification (LAMP) assay for the rapid detection of A. baumannii in clinical samples by using high-specificity primers of the bla(OXA-51) gene. Then we investigated the OXA-carbapenemases molecular epidemiology of A. baumannii isolates in two comprehensive hospitals in Beijing. The results showed that the LAMP assay could detect target DNA within 60 min at 65°C. The detection limit was 50 pg/μl, which was about 10-fold greater than that of PCR. Furthermore, this method could distinguish A. baumannii from the homologous A. nosocomialis and A. pittii. A total of 228 positive isolates were identified by this LAMP-based method for A. baumannii from 335 intensive care unit patients with clinically suspected multi-resistant infections in two hospitals in Beijing. The rates of CRAB are on the rise and are slowly becoming a routine phenotype for A. baumannii. Among the CRABs, 92.3% harbored both the bla(OXA-23) and bla(OXA-51) genes. Thirty-three pulsotypes were identified by pulsed-field gel electrophoresis, and the majority belonged to clone C. In conclusion, the LAMP method developed for detecting A. baumannii was faster and simpler than conventional PCR and has great potential for both point-of-care testing and basic research. We further demonstrated a high distribution of class D carbapenemase-encoding genes, mainly OXA-23, which presents an emerging threat in hospitals in China.
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spelling pubmed-45850702015-10-05 Rapid detection of Acinetobacter baumannii and molecular epidemiology of carbapenem-resistant A. baumannii in two comprehensive hospitals of Beijing, China Li, Puyuan Niu, Wenkai Li, Huan Lei, Hong Liu, Wei Zhao, Xiangna Guo, Leijing Zou, Dayang Yuan, Xin Liu, Huiying Yuan, Jing Bai, Changqing Front Microbiol Microbiology Acinetobacter baumannii is an important opportunistic pathogen associated with a variety of nosocomial infections. A rapid and sensitive molecular detection in clinical isolates is quite needed for the appropriate therapy and outbreak control of A. baumannii. Group 2 carbapenems have been considered the agents of choice for the treatment of multiple drug-resistant A. baumannii. But the prevalence of carbapenem-resistant A. baumannii (CRAB) has been steadily increasing in recent years. Here, we developed a loop-mediated isothermal amplification (LAMP) assay for the rapid detection of A. baumannii in clinical samples by using high-specificity primers of the bla(OXA-51) gene. Then we investigated the OXA-carbapenemases molecular epidemiology of A. baumannii isolates in two comprehensive hospitals in Beijing. The results showed that the LAMP assay could detect target DNA within 60 min at 65°C. The detection limit was 50 pg/μl, which was about 10-fold greater than that of PCR. Furthermore, this method could distinguish A. baumannii from the homologous A. nosocomialis and A. pittii. A total of 228 positive isolates were identified by this LAMP-based method for A. baumannii from 335 intensive care unit patients with clinically suspected multi-resistant infections in two hospitals in Beijing. The rates of CRAB are on the rise and are slowly becoming a routine phenotype for A. baumannii. Among the CRABs, 92.3% harbored both the bla(OXA-23) and bla(OXA-51) genes. Thirty-three pulsotypes were identified by pulsed-field gel electrophoresis, and the majority belonged to clone C. In conclusion, the LAMP method developed for detecting A. baumannii was faster and simpler than conventional PCR and has great potential for both point-of-care testing and basic research. We further demonstrated a high distribution of class D carbapenemase-encoding genes, mainly OXA-23, which presents an emerging threat in hospitals in China. Frontiers Media S.A. 2015-09-23 /pmc/articles/PMC4585070/ /pubmed/26441924 http://dx.doi.org/10.3389/fmicb.2015.00997 Text en Copyright © 2015 Li, Niu, Li, Lei, Liu, Zhao, Guo, Zou, Yuan, Liu, Yuan and Bai. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Li, Puyuan
Niu, Wenkai
Li, Huan
Lei, Hong
Liu, Wei
Zhao, Xiangna
Guo, Leijing
Zou, Dayang
Yuan, Xin
Liu, Huiying
Yuan, Jing
Bai, Changqing
Rapid detection of Acinetobacter baumannii and molecular epidemiology of carbapenem-resistant A. baumannii in two comprehensive hospitals of Beijing, China
title Rapid detection of Acinetobacter baumannii and molecular epidemiology of carbapenem-resistant A. baumannii in two comprehensive hospitals of Beijing, China
title_full Rapid detection of Acinetobacter baumannii and molecular epidemiology of carbapenem-resistant A. baumannii in two comprehensive hospitals of Beijing, China
title_fullStr Rapid detection of Acinetobacter baumannii and molecular epidemiology of carbapenem-resistant A. baumannii in two comprehensive hospitals of Beijing, China
title_full_unstemmed Rapid detection of Acinetobacter baumannii and molecular epidemiology of carbapenem-resistant A. baumannii in two comprehensive hospitals of Beijing, China
title_short Rapid detection of Acinetobacter baumannii and molecular epidemiology of carbapenem-resistant A. baumannii in two comprehensive hospitals of Beijing, China
title_sort rapid detection of acinetobacter baumannii and molecular epidemiology of carbapenem-resistant a. baumannii in two comprehensive hospitals of beijing, china
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4585070/
https://www.ncbi.nlm.nih.gov/pubmed/26441924
http://dx.doi.org/10.3389/fmicb.2015.00997
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