Cargando…
The novel cis-encoded antisense RNA AsrC positively regulates the expression of rpoE-rseABC operon and thus enhances the motility of Salmonella enterica serovar typhi
Bacterial non-coding RNAs are essential in many cellular processes, including response to environmental stress, and virulence. Deep sequencing analysis of the Salmonella enterica serovar typhi (S. typhi) transcriptome revealed a novel antisense RNA transcribed in cis on the strand complementary to r...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4585123/ https://www.ncbi.nlm.nih.gov/pubmed/26441919 http://dx.doi.org/10.3389/fmicb.2015.00990 |
_version_ | 1782392136488976384 |
---|---|
author | Zhang, Qi Zhang, Ying Zhang, Xiaolei Zhan, Lifang Zhao, Xin Xu, Shungao Sheng, Xiumei Huang, Xinxiang |
author_facet | Zhang, Qi Zhang, Ying Zhang, Xiaolei Zhan, Lifang Zhao, Xin Xu, Shungao Sheng, Xiumei Huang, Xinxiang |
author_sort | Zhang, Qi |
collection | PubMed |
description | Bacterial non-coding RNAs are essential in many cellular processes, including response to environmental stress, and virulence. Deep sequencing analysis of the Salmonella enterica serovar typhi (S. typhi) transcriptome revealed a novel antisense RNA transcribed in cis on the strand complementary to rseC, an activator gene of sigma factor RpoE. In this study, expression of this antisense RNA was confirmed in S. typhi by Northern hybridization. Rapid amplification of cDNA ends and sequence analysis identified an 893 bp sequence from the antisense RNA coding region that covered all of the rseC coding region in the reverse direction of transcription. This sequence of RNA was named as AsrC. After overexpression of AsrC with recombinantant plasmid in S. typhi, the bacterial motility was increased obviously. To explore the mechanism of AsrC function, regulation of rseC and rpoE expression by AsrC was investigated. We found that AsrC increased the levels of rseC mRNA and protein. The expression of rpoE was also increased in S. typhi after overexpression of AsrC, which was dependent on rseC. Thus, we propose that AsrC increased RseC level and indirectly activating RpoE which can initiate fliA expression and promote the motility of S. typhi. |
format | Online Article Text |
id | pubmed-4585123 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-45851232015-10-05 The novel cis-encoded antisense RNA AsrC positively regulates the expression of rpoE-rseABC operon and thus enhances the motility of Salmonella enterica serovar typhi Zhang, Qi Zhang, Ying Zhang, Xiaolei Zhan, Lifang Zhao, Xin Xu, Shungao Sheng, Xiumei Huang, Xinxiang Front Microbiol Microbiology Bacterial non-coding RNAs are essential in many cellular processes, including response to environmental stress, and virulence. Deep sequencing analysis of the Salmonella enterica serovar typhi (S. typhi) transcriptome revealed a novel antisense RNA transcribed in cis on the strand complementary to rseC, an activator gene of sigma factor RpoE. In this study, expression of this antisense RNA was confirmed in S. typhi by Northern hybridization. Rapid amplification of cDNA ends and sequence analysis identified an 893 bp sequence from the antisense RNA coding region that covered all of the rseC coding region in the reverse direction of transcription. This sequence of RNA was named as AsrC. After overexpression of AsrC with recombinantant plasmid in S. typhi, the bacterial motility was increased obviously. To explore the mechanism of AsrC function, regulation of rseC and rpoE expression by AsrC was investigated. We found that AsrC increased the levels of rseC mRNA and protein. The expression of rpoE was also increased in S. typhi after overexpression of AsrC, which was dependent on rseC. Thus, we propose that AsrC increased RseC level and indirectly activating RpoE which can initiate fliA expression and promote the motility of S. typhi. Frontiers Media S.A. 2015-09-17 /pmc/articles/PMC4585123/ /pubmed/26441919 http://dx.doi.org/10.3389/fmicb.2015.00990 Text en Copyright © 2015 Zhang, Zhang, Zhang, Zhan, Zhao, Xu, Sheng and Huang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Zhang, Qi Zhang, Ying Zhang, Xiaolei Zhan, Lifang Zhao, Xin Xu, Shungao Sheng, Xiumei Huang, Xinxiang The novel cis-encoded antisense RNA AsrC positively regulates the expression of rpoE-rseABC operon and thus enhances the motility of Salmonella enterica serovar typhi |
title | The novel cis-encoded antisense RNA AsrC positively regulates the expression of rpoE-rseABC operon and thus enhances the motility of Salmonella enterica serovar typhi |
title_full | The novel cis-encoded antisense RNA AsrC positively regulates the expression of rpoE-rseABC operon and thus enhances the motility of Salmonella enterica serovar typhi |
title_fullStr | The novel cis-encoded antisense RNA AsrC positively regulates the expression of rpoE-rseABC operon and thus enhances the motility of Salmonella enterica serovar typhi |
title_full_unstemmed | The novel cis-encoded antisense RNA AsrC positively regulates the expression of rpoE-rseABC operon and thus enhances the motility of Salmonella enterica serovar typhi |
title_short | The novel cis-encoded antisense RNA AsrC positively regulates the expression of rpoE-rseABC operon and thus enhances the motility of Salmonella enterica serovar typhi |
title_sort | novel cis-encoded antisense rna asrc positively regulates the expression of rpoe-rseabc operon and thus enhances the motility of salmonella enterica serovar typhi |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4585123/ https://www.ncbi.nlm.nih.gov/pubmed/26441919 http://dx.doi.org/10.3389/fmicb.2015.00990 |
work_keys_str_mv | AT zhangqi thenovelcisencodedantisensernaasrcpositivelyregulatestheexpressionofrpoerseabcoperonandthusenhancesthemotilityofsalmonellaentericaserovartyphi AT zhangying thenovelcisencodedantisensernaasrcpositivelyregulatestheexpressionofrpoerseabcoperonandthusenhancesthemotilityofsalmonellaentericaserovartyphi AT zhangxiaolei thenovelcisencodedantisensernaasrcpositivelyregulatestheexpressionofrpoerseabcoperonandthusenhancesthemotilityofsalmonellaentericaserovartyphi AT zhanlifang thenovelcisencodedantisensernaasrcpositivelyregulatestheexpressionofrpoerseabcoperonandthusenhancesthemotilityofsalmonellaentericaserovartyphi AT zhaoxin thenovelcisencodedantisensernaasrcpositivelyregulatestheexpressionofrpoerseabcoperonandthusenhancesthemotilityofsalmonellaentericaserovartyphi AT xushungao thenovelcisencodedantisensernaasrcpositivelyregulatestheexpressionofrpoerseabcoperonandthusenhancesthemotilityofsalmonellaentericaserovartyphi AT shengxiumei thenovelcisencodedantisensernaasrcpositivelyregulatestheexpressionofrpoerseabcoperonandthusenhancesthemotilityofsalmonellaentericaserovartyphi AT huangxinxiang thenovelcisencodedantisensernaasrcpositivelyregulatestheexpressionofrpoerseabcoperonandthusenhancesthemotilityofsalmonellaentericaserovartyphi AT zhangqi novelcisencodedantisensernaasrcpositivelyregulatestheexpressionofrpoerseabcoperonandthusenhancesthemotilityofsalmonellaentericaserovartyphi AT zhangying novelcisencodedantisensernaasrcpositivelyregulatestheexpressionofrpoerseabcoperonandthusenhancesthemotilityofsalmonellaentericaserovartyphi AT zhangxiaolei novelcisencodedantisensernaasrcpositivelyregulatestheexpressionofrpoerseabcoperonandthusenhancesthemotilityofsalmonellaentericaserovartyphi AT zhanlifang novelcisencodedantisensernaasrcpositivelyregulatestheexpressionofrpoerseabcoperonandthusenhancesthemotilityofsalmonellaentericaserovartyphi AT zhaoxin novelcisencodedantisensernaasrcpositivelyregulatestheexpressionofrpoerseabcoperonandthusenhancesthemotilityofsalmonellaentericaserovartyphi AT xushungao novelcisencodedantisensernaasrcpositivelyregulatestheexpressionofrpoerseabcoperonandthusenhancesthemotilityofsalmonellaentericaserovartyphi AT shengxiumei novelcisencodedantisensernaasrcpositivelyregulatestheexpressionofrpoerseabcoperonandthusenhancesthemotilityofsalmonellaentericaserovartyphi AT huangxinxiang novelcisencodedantisensernaasrcpositivelyregulatestheexpressionofrpoerseabcoperonandthusenhancesthemotilityofsalmonellaentericaserovartyphi |