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Genome-wide analysis and expression profiling under heat and drought treatments of HSP70 gene family in soybean (Glycine max L.)

Heat shock proteins (HSPs) perform a fundamental role in protecting plants against abiotic stresses. Previous studies have made great efforts in the functional analysis of individual family members, but there has not yet been an overall analysis or expression profiling of the HSP70 gene family in so...

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Autores principales: Zhang, Ling, Zhao, Hong-Kun, Dong, Qian-Li, Zhang, Yuan-Yu, Wang, Yu-Min, Li, Hai-Yun, Xing, Guo-Jie, Li, Qi-Yun, Dong, Ying-Shan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4585176/
https://www.ncbi.nlm.nih.gov/pubmed/26442082
http://dx.doi.org/10.3389/fpls.2015.00773
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author Zhang, Ling
Zhao, Hong-Kun
Dong, Qian-Li
Zhang, Yuan-Yu
Wang, Yu-Min
Li, Hai-Yun
Xing, Guo-Jie
Li, Qi-Yun
Dong, Ying-Shan
author_facet Zhang, Ling
Zhao, Hong-Kun
Dong, Qian-Li
Zhang, Yuan-Yu
Wang, Yu-Min
Li, Hai-Yun
Xing, Guo-Jie
Li, Qi-Yun
Dong, Ying-Shan
author_sort Zhang, Ling
collection PubMed
description Heat shock proteins (HSPs) perform a fundamental role in protecting plants against abiotic stresses. Previous studies have made great efforts in the functional analysis of individual family members, but there has not yet been an overall analysis or expression profiling of the HSP70 gene family in soybeans (Glycine max L.). In this study, an investigation of the soybean genome revealed 61 putative HSP70 genes, which were evaluated. These genes were classified into eight sub-families, denoted I–VIII, based on a phylogenetic analysis. In each sub-family, the constituent parts of the gene structure and motif were relatively conserved. These GmHSP70 genes were distributed unequally on 17 of the 20 chromosomes. The analysis of the expression profiles showed that 53 of the 61 GmHSP70 genes were differentially expressed across the 14 tissues. However, most of the GmHSP70s were differentially expressed in a tissue-specific expression pattern. Furthermore, the expression of some of the duplicate genes was partially redundant, while others showed functional diversity. The quantitative real-time PCR (qRT-PCR) analysis of the 61 soybean HSP70 genes confirmed their stress-inducible expression patterns under both drought and heat stress. These findings provide a thorough overview of the evolution and modification of the GmHSP70 gene family, which will help to determine the functional characteristics of the HSP70 genes in soybean growth and development.
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spelling pubmed-45851762015-10-05 Genome-wide analysis and expression profiling under heat and drought treatments of HSP70 gene family in soybean (Glycine max L.) Zhang, Ling Zhao, Hong-Kun Dong, Qian-Li Zhang, Yuan-Yu Wang, Yu-Min Li, Hai-Yun Xing, Guo-Jie Li, Qi-Yun Dong, Ying-Shan Front Plant Sci Plant Science Heat shock proteins (HSPs) perform a fundamental role in protecting plants against abiotic stresses. Previous studies have made great efforts in the functional analysis of individual family members, but there has not yet been an overall analysis or expression profiling of the HSP70 gene family in soybeans (Glycine max L.). In this study, an investigation of the soybean genome revealed 61 putative HSP70 genes, which were evaluated. These genes were classified into eight sub-families, denoted I–VIII, based on a phylogenetic analysis. In each sub-family, the constituent parts of the gene structure and motif were relatively conserved. These GmHSP70 genes were distributed unequally on 17 of the 20 chromosomes. The analysis of the expression profiles showed that 53 of the 61 GmHSP70 genes were differentially expressed across the 14 tissues. However, most of the GmHSP70s were differentially expressed in a tissue-specific expression pattern. Furthermore, the expression of some of the duplicate genes was partially redundant, while others showed functional diversity. The quantitative real-time PCR (qRT-PCR) analysis of the 61 soybean HSP70 genes confirmed their stress-inducible expression patterns under both drought and heat stress. These findings provide a thorough overview of the evolution and modification of the GmHSP70 gene family, which will help to determine the functional characteristics of the HSP70 genes in soybean growth and development. Frontiers Media S.A. 2015-09-25 /pmc/articles/PMC4585176/ /pubmed/26442082 http://dx.doi.org/10.3389/fpls.2015.00773 Text en Copyright © 2015 Zhang, Zhao, Dong, Zhang, Wang, Li, Xing, Li and Dong. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Zhang, Ling
Zhao, Hong-Kun
Dong, Qian-Li
Zhang, Yuan-Yu
Wang, Yu-Min
Li, Hai-Yun
Xing, Guo-Jie
Li, Qi-Yun
Dong, Ying-Shan
Genome-wide analysis and expression profiling under heat and drought treatments of HSP70 gene family in soybean (Glycine max L.)
title Genome-wide analysis and expression profiling under heat and drought treatments of HSP70 gene family in soybean (Glycine max L.)
title_full Genome-wide analysis and expression profiling under heat and drought treatments of HSP70 gene family in soybean (Glycine max L.)
title_fullStr Genome-wide analysis and expression profiling under heat and drought treatments of HSP70 gene family in soybean (Glycine max L.)
title_full_unstemmed Genome-wide analysis and expression profiling under heat and drought treatments of HSP70 gene family in soybean (Glycine max L.)
title_short Genome-wide analysis and expression profiling under heat and drought treatments of HSP70 gene family in soybean (Glycine max L.)
title_sort genome-wide analysis and expression profiling under heat and drought treatments of hsp70 gene family in soybean (glycine max l.)
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4585176/
https://www.ncbi.nlm.nih.gov/pubmed/26442082
http://dx.doi.org/10.3389/fpls.2015.00773
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