Cargando…
Evaluation on the effectiveness of 2-deoxyglucose-6-phosphate phosphatase (DOG(R)1) gene as a selectable marker for oil palm (Elaeis guineensis Jacq.) embryogenic calli transformation mediated by Agrobacterium tumefaciens
DOG(R)1, which encodes 2-deoxyglucose-6-phosphate phosphatase, has been used as a selectable marker gene to produce transgenic plants. In this study, a transformation vector, pBIDOG, which contains the DOG(R)1 gene, was transformed into oil palm embryogenic calli (EC) mediated by Agrobacterium tumef...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4585222/ https://www.ncbi.nlm.nih.gov/pubmed/26442041 http://dx.doi.org/10.3389/fpls.2015.00727 |
_version_ | 1782392159000854528 |
---|---|
author | Izawati, Abang Masli Dayang Masani, Mat Yunus Abdul Ismanizan, Ismail Parveez, Ghulam Kadir Ahmad |
author_facet | Izawati, Abang Masli Dayang Masani, Mat Yunus Abdul Ismanizan, Ismail Parveez, Ghulam Kadir Ahmad |
author_sort | Izawati, Abang Masli Dayang |
collection | PubMed |
description | DOG(R)1, which encodes 2-deoxyglucose-6-phosphate phosphatase, has been used as a selectable marker gene to produce transgenic plants. In this study, a transformation vector, pBIDOG, which contains the DOG(R)1 gene, was transformed into oil palm embryogenic calli (EC) mediated by Agrobacterium tumefaciens strain LBA4404. Transformed EC were exposed to 400 mg l(-1) 2-deoxyglucose (2-DOG) as the selection agent. 2-DOG resistant tissues were regenerated into whole plantlets on various regeneration media containing the same concentration of 2-DOG. The plantlets were later transferred into soil and grown in a biosafety screenhouse. PCR and subsequently Southern blot analyses were carried out to confirm the integration of the transgene in the plantlets. A transformation efficiency of about 1.0% was obtained using DOG(R)1 gene into the genome of oil palm. This result demonstrates the potential of using combination of DOG(R)1 gene and 2-DOG for regenerating transgenic oil palm. |
format | Online Article Text |
id | pubmed-4585222 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-45852222015-10-05 Evaluation on the effectiveness of 2-deoxyglucose-6-phosphate phosphatase (DOG(R)1) gene as a selectable marker for oil palm (Elaeis guineensis Jacq.) embryogenic calli transformation mediated by Agrobacterium tumefaciens Izawati, Abang Masli Dayang Masani, Mat Yunus Abdul Ismanizan, Ismail Parveez, Ghulam Kadir Ahmad Front Plant Sci Plant Science DOG(R)1, which encodes 2-deoxyglucose-6-phosphate phosphatase, has been used as a selectable marker gene to produce transgenic plants. In this study, a transformation vector, pBIDOG, which contains the DOG(R)1 gene, was transformed into oil palm embryogenic calli (EC) mediated by Agrobacterium tumefaciens strain LBA4404. Transformed EC were exposed to 400 mg l(-1) 2-deoxyglucose (2-DOG) as the selection agent. 2-DOG resistant tissues were regenerated into whole plantlets on various regeneration media containing the same concentration of 2-DOG. The plantlets were later transferred into soil and grown in a biosafety screenhouse. PCR and subsequently Southern blot analyses were carried out to confirm the integration of the transgene in the plantlets. A transformation efficiency of about 1.0% was obtained using DOG(R)1 gene into the genome of oil palm. This result demonstrates the potential of using combination of DOG(R)1 gene and 2-DOG for regenerating transgenic oil palm. Frontiers Media S.A. 2015-09-23 /pmc/articles/PMC4585222/ /pubmed/26442041 http://dx.doi.org/10.3389/fpls.2015.00727 Text en Copyright © 2015 Izawati, Masani, Ismanizan and Parveez. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Izawati, Abang Masli Dayang Masani, Mat Yunus Abdul Ismanizan, Ismail Parveez, Ghulam Kadir Ahmad Evaluation on the effectiveness of 2-deoxyglucose-6-phosphate phosphatase (DOG(R)1) gene as a selectable marker for oil palm (Elaeis guineensis Jacq.) embryogenic calli transformation mediated by Agrobacterium tumefaciens |
title | Evaluation on the effectiveness of 2-deoxyglucose-6-phosphate phosphatase (DOG(R)1) gene as a selectable marker for oil palm (Elaeis guineensis Jacq.) embryogenic calli transformation mediated by Agrobacterium tumefaciens |
title_full | Evaluation on the effectiveness of 2-deoxyglucose-6-phosphate phosphatase (DOG(R)1) gene as a selectable marker for oil palm (Elaeis guineensis Jacq.) embryogenic calli transformation mediated by Agrobacterium tumefaciens |
title_fullStr | Evaluation on the effectiveness of 2-deoxyglucose-6-phosphate phosphatase (DOG(R)1) gene as a selectable marker for oil palm (Elaeis guineensis Jacq.) embryogenic calli transformation mediated by Agrobacterium tumefaciens |
title_full_unstemmed | Evaluation on the effectiveness of 2-deoxyglucose-6-phosphate phosphatase (DOG(R)1) gene as a selectable marker for oil palm (Elaeis guineensis Jacq.) embryogenic calli transformation mediated by Agrobacterium tumefaciens |
title_short | Evaluation on the effectiveness of 2-deoxyglucose-6-phosphate phosphatase (DOG(R)1) gene as a selectable marker for oil palm (Elaeis guineensis Jacq.) embryogenic calli transformation mediated by Agrobacterium tumefaciens |
title_sort | evaluation on the effectiveness of 2-deoxyglucose-6-phosphate phosphatase (dog(r)1) gene as a selectable marker for oil palm (elaeis guineensis jacq.) embryogenic calli transformation mediated by agrobacterium tumefaciens |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4585222/ https://www.ncbi.nlm.nih.gov/pubmed/26442041 http://dx.doi.org/10.3389/fpls.2015.00727 |
work_keys_str_mv | AT izawatiabangmaslidayang evaluationontheeffectivenessof2deoxyglucose6phosphatephosphatasedogr1geneasaselectablemarkerforoilpalmelaeisguineensisjacqembryogeniccallitransformationmediatedbyagrobacteriumtumefaciens AT masanimatyunusabdul evaluationontheeffectivenessof2deoxyglucose6phosphatephosphatasedogr1geneasaselectablemarkerforoilpalmelaeisguineensisjacqembryogeniccallitransformationmediatedbyagrobacteriumtumefaciens AT ismanizanismail evaluationontheeffectivenessof2deoxyglucose6phosphatephosphatasedogr1geneasaselectablemarkerforoilpalmelaeisguineensisjacqembryogeniccallitransformationmediatedbyagrobacteriumtumefaciens AT parveezghulamkadirahmad evaluationontheeffectivenessof2deoxyglucose6phosphatephosphatasedogr1geneasaselectablemarkerforoilpalmelaeisguineensisjacqembryogeniccallitransformationmediatedbyagrobacteriumtumefaciens |