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Diversity of cultivable protease-producing bacteria in sediments of Jiaozhou Bay, China
Although protease-producing bacteria are key players in the degradation of organic nitrogen and essential for the nitrogen recycling in marine sediments, diversity of both these bacteria and their extracellular proteases is still largely unknown. This study investigated the diversity of the cultivab...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4585330/ https://www.ncbi.nlm.nih.gov/pubmed/26441943 http://dx.doi.org/10.3389/fmicb.2015.01021 |
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author | Zhang, Xi-Ying Han, Xiao-Xu Chen, Xiu-Lan Dang, Hong-Yue Xie, Bin-Bin Qin, Qi-Long Shi, Mei Zhou, Bai-Cheng Zhang, Yu-Zhong |
author_facet | Zhang, Xi-Ying Han, Xiao-Xu Chen, Xiu-Lan Dang, Hong-Yue Xie, Bin-Bin Qin, Qi-Long Shi, Mei Zhou, Bai-Cheng Zhang, Yu-Zhong |
author_sort | Zhang, Xi-Ying |
collection | PubMed |
description | Although protease-producing bacteria are key players in the degradation of organic nitrogen and essential for the nitrogen recycling in marine sediments, diversity of both these bacteria and their extracellular proteases is still largely unknown. This study investigated the diversity of the cultivable protease-producing bacteria and their extracellular proteases in the sediments of the eutrophied Jiaozhou Bay, China through phylogenetic analysis and protease inhibitor tests. The abundance of the cultivable protease-producing bacteria was up to 10(4) cells/g in all six sediment samples. The cultivated protease-producing bacteria mostly belonged to the phyla Proteobacteria and Firmicutes with the predominant genera being Photobacterium (39.4%), Bacillus (25.8%), and Vibrio (19.7%). Protease inhibitor tests revealed that extracellular proteases secreted by the bacteria were mainly serine proteases and/or metalloproteases with relatively low proportions of cysteine proteases. This study represents the first comprehensive analysis on the diversity of protease-producing bacteria and their extracellular proteases in sediments of a eutrophic bay. |
format | Online Article Text |
id | pubmed-4585330 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-45853302015-10-05 Diversity of cultivable protease-producing bacteria in sediments of Jiaozhou Bay, China Zhang, Xi-Ying Han, Xiao-Xu Chen, Xiu-Lan Dang, Hong-Yue Xie, Bin-Bin Qin, Qi-Long Shi, Mei Zhou, Bai-Cheng Zhang, Yu-Zhong Front Microbiol Microbiology Although protease-producing bacteria are key players in the degradation of organic nitrogen and essential for the nitrogen recycling in marine sediments, diversity of both these bacteria and their extracellular proteases is still largely unknown. This study investigated the diversity of the cultivable protease-producing bacteria and their extracellular proteases in the sediments of the eutrophied Jiaozhou Bay, China through phylogenetic analysis and protease inhibitor tests. The abundance of the cultivable protease-producing bacteria was up to 10(4) cells/g in all six sediment samples. The cultivated protease-producing bacteria mostly belonged to the phyla Proteobacteria and Firmicutes with the predominant genera being Photobacterium (39.4%), Bacillus (25.8%), and Vibrio (19.7%). Protease inhibitor tests revealed that extracellular proteases secreted by the bacteria were mainly serine proteases and/or metalloproteases with relatively low proportions of cysteine proteases. This study represents the first comprehensive analysis on the diversity of protease-producing bacteria and their extracellular proteases in sediments of a eutrophic bay. Frontiers Media S.A. 2015-09-25 /pmc/articles/PMC4585330/ /pubmed/26441943 http://dx.doi.org/10.3389/fmicb.2015.01021 Text en Copyright © 2015 Zhang, Han, Chen, Dang, Xie, Qin, Shi, Zhou and Zhang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Zhang, Xi-Ying Han, Xiao-Xu Chen, Xiu-Lan Dang, Hong-Yue Xie, Bin-Bin Qin, Qi-Long Shi, Mei Zhou, Bai-Cheng Zhang, Yu-Zhong Diversity of cultivable protease-producing bacteria in sediments of Jiaozhou Bay, China |
title | Diversity of cultivable protease-producing bacteria in sediments of Jiaozhou Bay, China |
title_full | Diversity of cultivable protease-producing bacteria in sediments of Jiaozhou Bay, China |
title_fullStr | Diversity of cultivable protease-producing bacteria in sediments of Jiaozhou Bay, China |
title_full_unstemmed | Diversity of cultivable protease-producing bacteria in sediments of Jiaozhou Bay, China |
title_short | Diversity of cultivable protease-producing bacteria in sediments of Jiaozhou Bay, China |
title_sort | diversity of cultivable protease-producing bacteria in sediments of jiaozhou bay, china |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4585330/ https://www.ncbi.nlm.nih.gov/pubmed/26441943 http://dx.doi.org/10.3389/fmicb.2015.01021 |
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