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Cortical and Clonal Contribution of Tbr2 Expressing Progenitors in the Developing Mouse Brain
The individual contribution of different progenitor subtypes towards the mature rodent cerebral cortex is not fully understood. Intermediate progenitor cells (IPCs) are key to understanding the regulation of neuronal number during cortical development and evolution, yet their exact contribution is m...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4585488/ https://www.ncbi.nlm.nih.gov/pubmed/24927931 http://dx.doi.org/10.1093/cercor/bhu125 |
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author | Vasistha, Navneet A. García-Moreno, Fernando Arora, Siddharth Cheung, Amanda F.P. Arnold, Sebastian J. Robertson, Elizabeth J. Molnár, Zoltán |
author_facet | Vasistha, Navneet A. García-Moreno, Fernando Arora, Siddharth Cheung, Amanda F.P. Arnold, Sebastian J. Robertson, Elizabeth J. Molnár, Zoltán |
author_sort | Vasistha, Navneet A. |
collection | PubMed |
description | The individual contribution of different progenitor subtypes towards the mature rodent cerebral cortex is not fully understood. Intermediate progenitor cells (IPCs) are key to understanding the regulation of neuronal number during cortical development and evolution, yet their exact contribution is much debated. Intermediate progenitors in the cortical subventricular zone are defined by expression of T-box brain-2 (Tbr2). In this study we demonstrate by using the Tbr2(Cre) mouse line and state-of-the-art cell lineage labeling techniques, that IPC derived cells contribute substantial proportions 67.5% of glutamatergic but not GABAergic or astrocytic cells to all cortical layers including the earliest generated subplate zone. We also describe the laminar dispersion of clonally derived cells from IPCs using a recently described clonal analysis tool (CLoNe) and show that pair-generated cells in different layers cluster closer (142.1 ± 76.8 μm) than unrelated cells (294.9 ± 105.4 μm). The clonal dispersion from individual Tbr2 positive intermediate progenitors contributes to increasing the cortical surface. Our study also describes extracortical contributions from Tbr2+ progenitors to the lateral olfactory tract and ventromedial hypothalamic nucleus. |
format | Online Article Text |
id | pubmed-4585488 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-45854882015-09-29 Cortical and Clonal Contribution of Tbr2 Expressing Progenitors in the Developing Mouse Brain Vasistha, Navneet A. García-Moreno, Fernando Arora, Siddharth Cheung, Amanda F.P. Arnold, Sebastian J. Robertson, Elizabeth J. Molnár, Zoltán Cereb Cortex Articles The individual contribution of different progenitor subtypes towards the mature rodent cerebral cortex is not fully understood. Intermediate progenitor cells (IPCs) are key to understanding the regulation of neuronal number during cortical development and evolution, yet their exact contribution is much debated. Intermediate progenitors in the cortical subventricular zone are defined by expression of T-box brain-2 (Tbr2). In this study we demonstrate by using the Tbr2(Cre) mouse line and state-of-the-art cell lineage labeling techniques, that IPC derived cells contribute substantial proportions 67.5% of glutamatergic but not GABAergic or astrocytic cells to all cortical layers including the earliest generated subplate zone. We also describe the laminar dispersion of clonally derived cells from IPCs using a recently described clonal analysis tool (CLoNe) and show that pair-generated cells in different layers cluster closer (142.1 ± 76.8 μm) than unrelated cells (294.9 ± 105.4 μm). The clonal dispersion from individual Tbr2 positive intermediate progenitors contributes to increasing the cortical surface. Our study also describes extracortical contributions from Tbr2+ progenitors to the lateral olfactory tract and ventromedial hypothalamic nucleus. Oxford University Press 2015-10 2014-06-13 /pmc/articles/PMC4585488/ /pubmed/24927931 http://dx.doi.org/10.1093/cercor/bhu125 Text en © The Author 2014. Published by Oxford University Press http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles Vasistha, Navneet A. García-Moreno, Fernando Arora, Siddharth Cheung, Amanda F.P. Arnold, Sebastian J. Robertson, Elizabeth J. Molnár, Zoltán Cortical and Clonal Contribution of Tbr2 Expressing Progenitors in the Developing Mouse Brain |
title | Cortical and Clonal Contribution of Tbr2 Expressing Progenitors in the Developing Mouse Brain |
title_full | Cortical and Clonal Contribution of Tbr2 Expressing Progenitors in the Developing Mouse Brain |
title_fullStr | Cortical and Clonal Contribution of Tbr2 Expressing Progenitors in the Developing Mouse Brain |
title_full_unstemmed | Cortical and Clonal Contribution of Tbr2 Expressing Progenitors in the Developing Mouse Brain |
title_short | Cortical and Clonal Contribution of Tbr2 Expressing Progenitors in the Developing Mouse Brain |
title_sort | cortical and clonal contribution of tbr2 expressing progenitors in the developing mouse brain |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4585488/ https://www.ncbi.nlm.nih.gov/pubmed/24927931 http://dx.doi.org/10.1093/cercor/bhu125 |
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