A Molecular Toolkit to Visualize Native Protein Assemblies in the Context of Human Disease

We present a new molecular toolkit to investigate protein assemblies natively formed in the context of human disease. The system employs tunable microchips that can be decorated with switchable adaptor molecules to select for target proteins of interest and analyze them using molecular microscopy. I...

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Detalles Bibliográficos
Autores principales: Gilmore, Brian L., Winton, Carly E., Demmert, Andrew C., Tanner, Justin R., Bowman, Sam, Karageorge, Vasilea, Patel, Kaya, Sheng, Zhi, Kelly, Deborah F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4585766/
https://www.ncbi.nlm.nih.gov/pubmed/26395823
http://dx.doi.org/10.1038/srep14440
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author Gilmore, Brian L.
Winton, Carly E.
Demmert, Andrew C.
Tanner, Justin R.
Bowman, Sam
Karageorge, Vasilea
Patel, Kaya
Sheng, Zhi
Kelly, Deborah F.
author_facet Gilmore, Brian L.
Winton, Carly E.
Demmert, Andrew C.
Tanner, Justin R.
Bowman, Sam
Karageorge, Vasilea
Patel, Kaya
Sheng, Zhi
Kelly, Deborah F.
author_sort Gilmore, Brian L.
collection PubMed
description We present a new molecular toolkit to investigate protein assemblies natively formed in the context of human disease. The system employs tunable microchips that can be decorated with switchable adaptor molecules to select for target proteins of interest and analyze them using molecular microscopy. Implementing our new streamlined microchip approach, we could directly visualize BRCA1 gene regulatory complexes from patient-derived cancer cells for the first time.
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spelling pubmed-45857662015-09-29 A Molecular Toolkit to Visualize Native Protein Assemblies in the Context of Human Disease Gilmore, Brian L. Winton, Carly E. Demmert, Andrew C. Tanner, Justin R. Bowman, Sam Karageorge, Vasilea Patel, Kaya Sheng, Zhi Kelly, Deborah F. Sci Rep Article We present a new molecular toolkit to investigate protein assemblies natively formed in the context of human disease. The system employs tunable microchips that can be decorated with switchable adaptor molecules to select for target proteins of interest and analyze them using molecular microscopy. Implementing our new streamlined microchip approach, we could directly visualize BRCA1 gene regulatory complexes from patient-derived cancer cells for the first time. Nature Publishing Group 2015-09-23 /pmc/articles/PMC4585766/ /pubmed/26395823 http://dx.doi.org/10.1038/srep14440 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Gilmore, Brian L.
Winton, Carly E.
Demmert, Andrew C.
Tanner, Justin R.
Bowman, Sam
Karageorge, Vasilea
Patel, Kaya
Sheng, Zhi
Kelly, Deborah F.
A Molecular Toolkit to Visualize Native Protein Assemblies in the Context of Human Disease
title A Molecular Toolkit to Visualize Native Protein Assemblies in the Context of Human Disease
title_full A Molecular Toolkit to Visualize Native Protein Assemblies in the Context of Human Disease
title_fullStr A Molecular Toolkit to Visualize Native Protein Assemblies in the Context of Human Disease
title_full_unstemmed A Molecular Toolkit to Visualize Native Protein Assemblies in the Context of Human Disease
title_short A Molecular Toolkit to Visualize Native Protein Assemblies in the Context of Human Disease
title_sort molecular toolkit to visualize native protein assemblies in the context of human disease
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4585766/
https://www.ncbi.nlm.nih.gov/pubmed/26395823
http://dx.doi.org/10.1038/srep14440
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