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Comparative quantitative trait loci for silique length and seed weight in Brassica napus

Silique length (SL) and seed weight (SW) are important yield-associated traits in rapeseed (Brassica napus). Although many quantitative trait loci (QTL) for SL and SW have been identified in B. napus, comparative analysis for those QTL is seldom performed. In the present study, 20 and 21 QTL for SL...

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Autores principales: Fu, Ying, Wei, Dayong, Dong, Hongli, He, Yajun, Cui, Yixin, Mei, Jiaqin, Wan, Huafang, Li, Jiana, Snowdon, Rod, Friedt, Wolfgang, Li, Xiaorong, Qian, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4585775/
https://www.ncbi.nlm.nih.gov/pubmed/26394547
http://dx.doi.org/10.1038/srep14407
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author Fu, Ying
Wei, Dayong
Dong, Hongli
He, Yajun
Cui, Yixin
Mei, Jiaqin
Wan, Huafang
Li, Jiana
Snowdon, Rod
Friedt, Wolfgang
Li, Xiaorong
Qian, Wei
author_facet Fu, Ying
Wei, Dayong
Dong, Hongli
He, Yajun
Cui, Yixin
Mei, Jiaqin
Wan, Huafang
Li, Jiana
Snowdon, Rod
Friedt, Wolfgang
Li, Xiaorong
Qian, Wei
author_sort Fu, Ying
collection PubMed
description Silique length (SL) and seed weight (SW) are important yield-associated traits in rapeseed (Brassica napus). Although many quantitative trait loci (QTL) for SL and SW have been identified in B. napus, comparative analysis for those QTL is seldom performed. In the present study, 20 and 21 QTL for SL and SW were identified in doubled haploid (DH) and DH-derived reconstructed F(2) populations in rapeseed, explaining 55.1–74.3% and 24.4–62.9% of the phenotypic variation across three years, respectively. Of which, 17 QTL with partially or completely overlapped confidence interval on chromosome A09, were homologous with two overlapped QTL on chromosome C08 by aligning QTL confidence intervals with the reference genomes of Brassica crops. By high density selective genotyping of DH lines with extreme phenotypes, using a Brassica single-nucleotide polymorphism (SNP) array, the QTL on chromosome A09 was narrowed, and aligned into 1.14-Mb region from 30.84 to 31.98 Mb on chromosome R09 of B. rapa and 1.05-Mb region from 27.21 to 28.26 Mb on chromosome A09 of B. napus. The alignment of QTL with Brassica reference genomes revealed homologous QTL on A09 and C08 for SL. The narrowed QTL region provides clues for gene cloning and breeding cultivars by marker-assisted selection.
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spelling pubmed-45857752015-09-29 Comparative quantitative trait loci for silique length and seed weight in Brassica napus Fu, Ying Wei, Dayong Dong, Hongli He, Yajun Cui, Yixin Mei, Jiaqin Wan, Huafang Li, Jiana Snowdon, Rod Friedt, Wolfgang Li, Xiaorong Qian, Wei Sci Rep Article Silique length (SL) and seed weight (SW) are important yield-associated traits in rapeseed (Brassica napus). Although many quantitative trait loci (QTL) for SL and SW have been identified in B. napus, comparative analysis for those QTL is seldom performed. In the present study, 20 and 21 QTL for SL and SW were identified in doubled haploid (DH) and DH-derived reconstructed F(2) populations in rapeseed, explaining 55.1–74.3% and 24.4–62.9% of the phenotypic variation across three years, respectively. Of which, 17 QTL with partially or completely overlapped confidence interval on chromosome A09, were homologous with two overlapped QTL on chromosome C08 by aligning QTL confidence intervals with the reference genomes of Brassica crops. By high density selective genotyping of DH lines with extreme phenotypes, using a Brassica single-nucleotide polymorphism (SNP) array, the QTL on chromosome A09 was narrowed, and aligned into 1.14-Mb region from 30.84 to 31.98 Mb on chromosome R09 of B. rapa and 1.05-Mb region from 27.21 to 28.26 Mb on chromosome A09 of B. napus. The alignment of QTL with Brassica reference genomes revealed homologous QTL on A09 and C08 for SL. The narrowed QTL region provides clues for gene cloning and breeding cultivars by marker-assisted selection. Nature Publishing Group 2015-09-23 /pmc/articles/PMC4585775/ /pubmed/26394547 http://dx.doi.org/10.1038/srep14407 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Fu, Ying
Wei, Dayong
Dong, Hongli
He, Yajun
Cui, Yixin
Mei, Jiaqin
Wan, Huafang
Li, Jiana
Snowdon, Rod
Friedt, Wolfgang
Li, Xiaorong
Qian, Wei
Comparative quantitative trait loci for silique length and seed weight in Brassica napus
title Comparative quantitative trait loci for silique length and seed weight in Brassica napus
title_full Comparative quantitative trait loci for silique length and seed weight in Brassica napus
title_fullStr Comparative quantitative trait loci for silique length and seed weight in Brassica napus
title_full_unstemmed Comparative quantitative trait loci for silique length and seed weight in Brassica napus
title_short Comparative quantitative trait loci for silique length and seed weight in Brassica napus
title_sort comparative quantitative trait loci for silique length and seed weight in brassica napus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4585775/
https://www.ncbi.nlm.nih.gov/pubmed/26394547
http://dx.doi.org/10.1038/srep14407
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