QIAD assay for quantitating a compound’s efficacy in elimination of toxic Aβ oligomers

Strong evidence exists for a central role of amyloid β-protein (Aβ) oligomers in the pathogenesis of Alzheimer’s disease. We have developed a fast, reliable and robust in vitro assay, termed QIAD, to quantify the effect of any compound on the Aβ aggregate size distribution. Applying QIAD, we studied...

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Autores principales: Brener, Oleksandr, Dunkelmann, Tina, Gremer, Lothar, van Groen, Thomas, Mirecka, Ewa A., Kadish, Inga, Willuweit, Antje, Kutzsche, Janine, Jürgens, Dagmar, Rudolph, Stephan, Tusche, Markus, Bongen, Patrick, Pietruszka, Jörg, Oesterhelt, Filipp, Langen, Karl-Josef, Demuth, Hans-Ulrich, Janssen, Arnold, Hoyer, Wolfgang, Funke, Susanne A., Nagel-Steger, Luitgard, Willbold, Dieter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4585794/
https://www.ncbi.nlm.nih.gov/pubmed/26394756
http://dx.doi.org/10.1038/srep13222
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author Brener, Oleksandr
Dunkelmann, Tina
Gremer, Lothar
van Groen, Thomas
Mirecka, Ewa A.
Kadish, Inga
Willuweit, Antje
Kutzsche, Janine
Jürgens, Dagmar
Rudolph, Stephan
Tusche, Markus
Bongen, Patrick
Pietruszka, Jörg
Oesterhelt, Filipp
Langen, Karl-Josef
Demuth, Hans-Ulrich
Janssen, Arnold
Hoyer, Wolfgang
Funke, Susanne A.
Nagel-Steger, Luitgard
Willbold, Dieter
author_facet Brener, Oleksandr
Dunkelmann, Tina
Gremer, Lothar
van Groen, Thomas
Mirecka, Ewa A.
Kadish, Inga
Willuweit, Antje
Kutzsche, Janine
Jürgens, Dagmar
Rudolph, Stephan
Tusche, Markus
Bongen, Patrick
Pietruszka, Jörg
Oesterhelt, Filipp
Langen, Karl-Josef
Demuth, Hans-Ulrich
Janssen, Arnold
Hoyer, Wolfgang
Funke, Susanne A.
Nagel-Steger, Luitgard
Willbold, Dieter
author_sort Brener, Oleksandr
collection PubMed
description Strong evidence exists for a central role of amyloid β-protein (Aβ) oligomers in the pathogenesis of Alzheimer’s disease. We have developed a fast, reliable and robust in vitro assay, termed QIAD, to quantify the effect of any compound on the Aβ aggregate size distribution. Applying QIAD, we studied the effect of homotaurine, scyllo-inositol, EGCG, the benzofuran derivative KMS88009, ZAβ3W, the D-enantiomeric peptide D3 and its tandem version D3D3 on Aβ aggregation. The predictive power of the assay for in vivo efficacy is demonstrated by comparing the oligomer elimination efficiency of D3 and D3D3 with their treatment effects in animal models of Alzheimer´s disease.
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spelling pubmed-45857942015-09-29 QIAD assay for quantitating a compound’s efficacy in elimination of toxic Aβ oligomers Brener, Oleksandr Dunkelmann, Tina Gremer, Lothar van Groen, Thomas Mirecka, Ewa A. Kadish, Inga Willuweit, Antje Kutzsche, Janine Jürgens, Dagmar Rudolph, Stephan Tusche, Markus Bongen, Patrick Pietruszka, Jörg Oesterhelt, Filipp Langen, Karl-Josef Demuth, Hans-Ulrich Janssen, Arnold Hoyer, Wolfgang Funke, Susanne A. Nagel-Steger, Luitgard Willbold, Dieter Sci Rep Article Strong evidence exists for a central role of amyloid β-protein (Aβ) oligomers in the pathogenesis of Alzheimer’s disease. We have developed a fast, reliable and robust in vitro assay, termed QIAD, to quantify the effect of any compound on the Aβ aggregate size distribution. Applying QIAD, we studied the effect of homotaurine, scyllo-inositol, EGCG, the benzofuran derivative KMS88009, ZAβ3W, the D-enantiomeric peptide D3 and its tandem version D3D3 on Aβ aggregation. The predictive power of the assay for in vivo efficacy is demonstrated by comparing the oligomer elimination efficiency of D3 and D3D3 with their treatment effects in animal models of Alzheimer´s disease. Nature Publishing Group 2015-09-23 /pmc/articles/PMC4585794/ /pubmed/26394756 http://dx.doi.org/10.1038/srep13222 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Brener, Oleksandr
Dunkelmann, Tina
Gremer, Lothar
van Groen, Thomas
Mirecka, Ewa A.
Kadish, Inga
Willuweit, Antje
Kutzsche, Janine
Jürgens, Dagmar
Rudolph, Stephan
Tusche, Markus
Bongen, Patrick
Pietruszka, Jörg
Oesterhelt, Filipp
Langen, Karl-Josef
Demuth, Hans-Ulrich
Janssen, Arnold
Hoyer, Wolfgang
Funke, Susanne A.
Nagel-Steger, Luitgard
Willbold, Dieter
QIAD assay for quantitating a compound’s efficacy in elimination of toxic Aβ oligomers
title QIAD assay for quantitating a compound’s efficacy in elimination of toxic Aβ oligomers
title_full QIAD assay for quantitating a compound’s efficacy in elimination of toxic Aβ oligomers
title_fullStr QIAD assay for quantitating a compound’s efficacy in elimination of toxic Aβ oligomers
title_full_unstemmed QIAD assay for quantitating a compound’s efficacy in elimination of toxic Aβ oligomers
title_short QIAD assay for quantitating a compound’s efficacy in elimination of toxic Aβ oligomers
title_sort qiad assay for quantitating a compound’s efficacy in elimination of toxic aβ oligomers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4585794/
https://www.ncbi.nlm.nih.gov/pubmed/26394756
http://dx.doi.org/10.1038/srep13222
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