Recombinant human thrombopoietin promotes hematopoietic reconstruction after severe whole body irradiation

Recombinant human thrombopoietin (rHuTPO) is a drug that is used clinically to promote megakaryocyte and platelet generation. Here, we report the mitigative effect of rHuTPO (administered after exposure) against severe whole body irradiation in mice. Injection of rHuTPO for 14 consecutive days follo...

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Autores principales: Wang, Chao, Zhang, Bowen, Wang, Sihan, Zhang, Jing, Liu, Yiming, Wang, Jingxue, Fan, Zeng, Lv, Yang, Zhang, Xiuyuan, He, Lijuan, Chen, Lin, Xia, Huanzhang, Li, Yanhua, Pei, Xuetao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4585893/
https://www.ncbi.nlm.nih.gov/pubmed/26403418
http://dx.doi.org/10.1038/srep12993
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author Wang, Chao
Zhang, Bowen
Wang, Sihan
Zhang, Jing
Liu, Yiming
Wang, Jingxue
Fan, Zeng
Lv, Yang
Zhang, Xiuyuan
He, Lijuan
Chen, Lin
Xia, Huanzhang
Li, Yanhua
Pei, Xuetao
author_facet Wang, Chao
Zhang, Bowen
Wang, Sihan
Zhang, Jing
Liu, Yiming
Wang, Jingxue
Fan, Zeng
Lv, Yang
Zhang, Xiuyuan
He, Lijuan
Chen, Lin
Xia, Huanzhang
Li, Yanhua
Pei, Xuetao
author_sort Wang, Chao
collection PubMed
description Recombinant human thrombopoietin (rHuTPO) is a drug that is used clinically to promote megakaryocyte and platelet generation. Here, we report the mitigative effect of rHuTPO (administered after exposure) against severe whole body irradiation in mice. Injection of rHuTPO for 14 consecutive days following exposure significantly improved the survival rate of lethally irradiated mice. RHuTPO treatment notably increased bone marrow cell density and LSK cell numbers in the mice after sub-lethal irradiation primarily by promoting residual HSC proliferation. In lethally irradiated mice with hematopoietic cell transplantation, rHuTPO treatment increased the survival rate and enhanced hematopoietic cell engraftment compared with the placebo treatment. Our observations indicate that recombinant human TPO might have a therapeutic role in promoting hematopoietic reconstitution and HSC engraftment.
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spelling pubmed-45858932015-09-30 Recombinant human thrombopoietin promotes hematopoietic reconstruction after severe whole body irradiation Wang, Chao Zhang, Bowen Wang, Sihan Zhang, Jing Liu, Yiming Wang, Jingxue Fan, Zeng Lv, Yang Zhang, Xiuyuan He, Lijuan Chen, Lin Xia, Huanzhang Li, Yanhua Pei, Xuetao Sci Rep Article Recombinant human thrombopoietin (rHuTPO) is a drug that is used clinically to promote megakaryocyte and platelet generation. Here, we report the mitigative effect of rHuTPO (administered after exposure) against severe whole body irradiation in mice. Injection of rHuTPO for 14 consecutive days following exposure significantly improved the survival rate of lethally irradiated mice. RHuTPO treatment notably increased bone marrow cell density and LSK cell numbers in the mice after sub-lethal irradiation primarily by promoting residual HSC proliferation. In lethally irradiated mice with hematopoietic cell transplantation, rHuTPO treatment increased the survival rate and enhanced hematopoietic cell engraftment compared with the placebo treatment. Our observations indicate that recombinant human TPO might have a therapeutic role in promoting hematopoietic reconstitution and HSC engraftment. Nature Publishing Group 2015-09-25 /pmc/articles/PMC4585893/ /pubmed/26403418 http://dx.doi.org/10.1038/srep12993 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Wang, Chao
Zhang, Bowen
Wang, Sihan
Zhang, Jing
Liu, Yiming
Wang, Jingxue
Fan, Zeng
Lv, Yang
Zhang, Xiuyuan
He, Lijuan
Chen, Lin
Xia, Huanzhang
Li, Yanhua
Pei, Xuetao
Recombinant human thrombopoietin promotes hematopoietic reconstruction after severe whole body irradiation
title Recombinant human thrombopoietin promotes hematopoietic reconstruction after severe whole body irradiation
title_full Recombinant human thrombopoietin promotes hematopoietic reconstruction after severe whole body irradiation
title_fullStr Recombinant human thrombopoietin promotes hematopoietic reconstruction after severe whole body irradiation
title_full_unstemmed Recombinant human thrombopoietin promotes hematopoietic reconstruction after severe whole body irradiation
title_short Recombinant human thrombopoietin promotes hematopoietic reconstruction after severe whole body irradiation
title_sort recombinant human thrombopoietin promotes hematopoietic reconstruction after severe whole body irradiation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4585893/
https://www.ncbi.nlm.nih.gov/pubmed/26403418
http://dx.doi.org/10.1038/srep12993
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