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CRISP1 as a novel CatSper regulator that modulates sperm motility and orientation during fertilization

Ca(2+)-dependent mechanisms are critical for successful completion of fertilization. Here, we demonstrate that CRISP1, a sperm protein involved in mammalian fertilization, is also present in the female gamete and capable of modulating key sperm Ca(2+) channels. Specifically, we show that CRISP1 is e...

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Autores principales: Ernesto, Juan I., Weigel Muñoz, Mariana, Battistone, María A., Vasen, Gustavo, Martínez-López, Pablo, Orta, Gerardo, Figueiras-Fierro, Dulce, De la Vega-Beltran, José L., Moreno, Ignacio A., Guidobaldi, Héctor A., Giojalas, Laura, Darszon, Alberto, Cohen, Débora J., Cuasnicú, Patricia S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4586743/
https://www.ncbi.nlm.nih.gov/pubmed/26416967
http://dx.doi.org/10.1083/jcb.201412041
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author Ernesto, Juan I.
Weigel Muñoz, Mariana
Battistone, María A.
Vasen, Gustavo
Martínez-López, Pablo
Orta, Gerardo
Figueiras-Fierro, Dulce
De la Vega-Beltran, José L.
Moreno, Ignacio A.
Guidobaldi, Héctor A.
Giojalas, Laura
Darszon, Alberto
Cohen, Débora J.
Cuasnicú, Patricia S.
author_facet Ernesto, Juan I.
Weigel Muñoz, Mariana
Battistone, María A.
Vasen, Gustavo
Martínez-López, Pablo
Orta, Gerardo
Figueiras-Fierro, Dulce
De la Vega-Beltran, José L.
Moreno, Ignacio A.
Guidobaldi, Héctor A.
Giojalas, Laura
Darszon, Alberto
Cohen, Débora J.
Cuasnicú, Patricia S.
author_sort Ernesto, Juan I.
collection PubMed
description Ca(2+)-dependent mechanisms are critical for successful completion of fertilization. Here, we demonstrate that CRISP1, a sperm protein involved in mammalian fertilization, is also present in the female gamete and capable of modulating key sperm Ca(2+) channels. Specifically, we show that CRISP1 is expressed by the cumulus cells that surround the egg and that fertilization of cumulus–oocyte complexes from CRISP1 knockout females is impaired because of a failure of sperm to penetrate the cumulus. We provide evidence that CRISP1 stimulates sperm orientation by modulating sperm hyperactivation, a vigorous motility required for penetration of the egg vestments. Moreover, patch clamping of sperm revealed that CRISP1 has the ability to regulate CatSper, the principal sperm Ca(2+) channel involved in hyperactivation and essential for fertility. Given the critical role of Ca(2+) for sperm motility, we propose a novel CRISP1-mediated fine-tuning mechanism to regulate sperm hyperactivation and orientation for successful penetration of the cumulus during fertilization.
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spelling pubmed-45867432016-03-28 CRISP1 as a novel CatSper regulator that modulates sperm motility and orientation during fertilization Ernesto, Juan I. Weigel Muñoz, Mariana Battistone, María A. Vasen, Gustavo Martínez-López, Pablo Orta, Gerardo Figueiras-Fierro, Dulce De la Vega-Beltran, José L. Moreno, Ignacio A. Guidobaldi, Héctor A. Giojalas, Laura Darszon, Alberto Cohen, Débora J. Cuasnicú, Patricia S. J Cell Biol Research Articles Ca(2+)-dependent mechanisms are critical for successful completion of fertilization. Here, we demonstrate that CRISP1, a sperm protein involved in mammalian fertilization, is also present in the female gamete and capable of modulating key sperm Ca(2+) channels. Specifically, we show that CRISP1 is expressed by the cumulus cells that surround the egg and that fertilization of cumulus–oocyte complexes from CRISP1 knockout females is impaired because of a failure of sperm to penetrate the cumulus. We provide evidence that CRISP1 stimulates sperm orientation by modulating sperm hyperactivation, a vigorous motility required for penetration of the egg vestments. Moreover, patch clamping of sperm revealed that CRISP1 has the ability to regulate CatSper, the principal sperm Ca(2+) channel involved in hyperactivation and essential for fertility. Given the critical role of Ca(2+) for sperm motility, we propose a novel CRISP1-mediated fine-tuning mechanism to regulate sperm hyperactivation and orientation for successful penetration of the cumulus during fertilization. The Rockefeller University Press 2015-09-28 /pmc/articles/PMC4586743/ /pubmed/26416967 http://dx.doi.org/10.1083/jcb.201412041 Text en © 2015 Ernesto et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Ernesto, Juan I.
Weigel Muñoz, Mariana
Battistone, María A.
Vasen, Gustavo
Martínez-López, Pablo
Orta, Gerardo
Figueiras-Fierro, Dulce
De la Vega-Beltran, José L.
Moreno, Ignacio A.
Guidobaldi, Héctor A.
Giojalas, Laura
Darszon, Alberto
Cohen, Débora J.
Cuasnicú, Patricia S.
CRISP1 as a novel CatSper regulator that modulates sperm motility and orientation during fertilization
title CRISP1 as a novel CatSper regulator that modulates sperm motility and orientation during fertilization
title_full CRISP1 as a novel CatSper regulator that modulates sperm motility and orientation during fertilization
title_fullStr CRISP1 as a novel CatSper regulator that modulates sperm motility and orientation during fertilization
title_full_unstemmed CRISP1 as a novel CatSper regulator that modulates sperm motility and orientation during fertilization
title_short CRISP1 as a novel CatSper regulator that modulates sperm motility and orientation during fertilization
title_sort crisp1 as a novel catsper regulator that modulates sperm motility and orientation during fertilization
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4586743/
https://www.ncbi.nlm.nih.gov/pubmed/26416967
http://dx.doi.org/10.1083/jcb.201412041
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