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CRISP1 as a novel CatSper regulator that modulates sperm motility and orientation during fertilization
Ca(2+)-dependent mechanisms are critical for successful completion of fertilization. Here, we demonstrate that CRISP1, a sperm protein involved in mammalian fertilization, is also present in the female gamete and capable of modulating key sperm Ca(2+) channels. Specifically, we show that CRISP1 is e...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4586743/ https://www.ncbi.nlm.nih.gov/pubmed/26416967 http://dx.doi.org/10.1083/jcb.201412041 |
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author | Ernesto, Juan I. Weigel Muñoz, Mariana Battistone, María A. Vasen, Gustavo Martínez-López, Pablo Orta, Gerardo Figueiras-Fierro, Dulce De la Vega-Beltran, José L. Moreno, Ignacio A. Guidobaldi, Héctor A. Giojalas, Laura Darszon, Alberto Cohen, Débora J. Cuasnicú, Patricia S. |
author_facet | Ernesto, Juan I. Weigel Muñoz, Mariana Battistone, María A. Vasen, Gustavo Martínez-López, Pablo Orta, Gerardo Figueiras-Fierro, Dulce De la Vega-Beltran, José L. Moreno, Ignacio A. Guidobaldi, Héctor A. Giojalas, Laura Darszon, Alberto Cohen, Débora J. Cuasnicú, Patricia S. |
author_sort | Ernesto, Juan I. |
collection | PubMed |
description | Ca(2+)-dependent mechanisms are critical for successful completion of fertilization. Here, we demonstrate that CRISP1, a sperm protein involved in mammalian fertilization, is also present in the female gamete and capable of modulating key sperm Ca(2+) channels. Specifically, we show that CRISP1 is expressed by the cumulus cells that surround the egg and that fertilization of cumulus–oocyte complexes from CRISP1 knockout females is impaired because of a failure of sperm to penetrate the cumulus. We provide evidence that CRISP1 stimulates sperm orientation by modulating sperm hyperactivation, a vigorous motility required for penetration of the egg vestments. Moreover, patch clamping of sperm revealed that CRISP1 has the ability to regulate CatSper, the principal sperm Ca(2+) channel involved in hyperactivation and essential for fertility. Given the critical role of Ca(2+) for sperm motility, we propose a novel CRISP1-mediated fine-tuning mechanism to regulate sperm hyperactivation and orientation for successful penetration of the cumulus during fertilization. |
format | Online Article Text |
id | pubmed-4586743 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-45867432016-03-28 CRISP1 as a novel CatSper regulator that modulates sperm motility and orientation during fertilization Ernesto, Juan I. Weigel Muñoz, Mariana Battistone, María A. Vasen, Gustavo Martínez-López, Pablo Orta, Gerardo Figueiras-Fierro, Dulce De la Vega-Beltran, José L. Moreno, Ignacio A. Guidobaldi, Héctor A. Giojalas, Laura Darszon, Alberto Cohen, Débora J. Cuasnicú, Patricia S. J Cell Biol Research Articles Ca(2+)-dependent mechanisms are critical for successful completion of fertilization. Here, we demonstrate that CRISP1, a sperm protein involved in mammalian fertilization, is also present in the female gamete and capable of modulating key sperm Ca(2+) channels. Specifically, we show that CRISP1 is expressed by the cumulus cells that surround the egg and that fertilization of cumulus–oocyte complexes from CRISP1 knockout females is impaired because of a failure of sperm to penetrate the cumulus. We provide evidence that CRISP1 stimulates sperm orientation by modulating sperm hyperactivation, a vigorous motility required for penetration of the egg vestments. Moreover, patch clamping of sperm revealed that CRISP1 has the ability to regulate CatSper, the principal sperm Ca(2+) channel involved in hyperactivation and essential for fertility. Given the critical role of Ca(2+) for sperm motility, we propose a novel CRISP1-mediated fine-tuning mechanism to regulate sperm hyperactivation and orientation for successful penetration of the cumulus during fertilization. The Rockefeller University Press 2015-09-28 /pmc/articles/PMC4586743/ /pubmed/26416967 http://dx.doi.org/10.1083/jcb.201412041 Text en © 2015 Ernesto et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Ernesto, Juan I. Weigel Muñoz, Mariana Battistone, María A. Vasen, Gustavo Martínez-López, Pablo Orta, Gerardo Figueiras-Fierro, Dulce De la Vega-Beltran, José L. Moreno, Ignacio A. Guidobaldi, Héctor A. Giojalas, Laura Darszon, Alberto Cohen, Débora J. Cuasnicú, Patricia S. CRISP1 as a novel CatSper regulator that modulates sperm motility and orientation during fertilization |
title | CRISP1 as a novel CatSper regulator that modulates sperm motility and orientation during fertilization |
title_full | CRISP1 as a novel CatSper regulator that modulates sperm motility and orientation during fertilization |
title_fullStr | CRISP1 as a novel CatSper regulator that modulates sperm motility and orientation during fertilization |
title_full_unstemmed | CRISP1 as a novel CatSper regulator that modulates sperm motility and orientation during fertilization |
title_short | CRISP1 as a novel CatSper regulator that modulates sperm motility and orientation during fertilization |
title_sort | crisp1 as a novel catsper regulator that modulates sperm motility and orientation during fertilization |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4586743/ https://www.ncbi.nlm.nih.gov/pubmed/26416967 http://dx.doi.org/10.1083/jcb.201412041 |
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