Revealing Annexin A2 and ARF-6 enrollment during Trypanosoma cruzi extracellular amastigote-host cell interaction

BACKGROUND: Invasion of host cells by Trypanosoma cruzi extracellular amastigotes is host actin polymerization-dependent. However, the role of proteins related to actin dynamics during invasion by amastigotes remains to be investigated. Here we describe the role of Annexin A2 and ARF-6 during extrac...

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Autores principales: Teixeira, Thaise Lara, Cruz, Lilian, Mortara, Renato Arruda, Da Silva, Claudio Vieira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4587755/
https://www.ncbi.nlm.nih.gov/pubmed/26416603
http://dx.doi.org/10.1186/s13071-015-1097-6
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author Teixeira, Thaise Lara
Cruz, Lilian
Mortara, Renato Arruda
Da Silva, Claudio Vieira
author_facet Teixeira, Thaise Lara
Cruz, Lilian
Mortara, Renato Arruda
Da Silva, Claudio Vieira
author_sort Teixeira, Thaise Lara
collection PubMed
description BACKGROUND: Invasion of host cells by Trypanosoma cruzi extracellular amastigotes is host actin polymerization-dependent. However, the role of proteins related to actin dynamics during invasion by amastigotes remains to be investigated. Here we describe the role of Annexin A2 and ARF-6 during extracellular amastigote-mammalian cell interactions. FINDINGS: Our results showed ARF-6 accumulation in the amastigote-containing parasitophorous vacuole containing amastigote forms; demonstrated ARF-6 and Annexin A2 critical impact over parasite cell invasion and revealed the effect of Annexin A2 expression on intracellular parasite multiplication. CONCLUSION: ARF-6 and Annexin A2 are involved in invasion of mammalian cells by T. cruzi amastigotes.
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spelling pubmed-45877552015-09-30 Revealing Annexin A2 and ARF-6 enrollment during Trypanosoma cruzi extracellular amastigote-host cell interaction Teixeira, Thaise Lara Cruz, Lilian Mortara, Renato Arruda Da Silva, Claudio Vieira Parasit Vectors Short Report BACKGROUND: Invasion of host cells by Trypanosoma cruzi extracellular amastigotes is host actin polymerization-dependent. However, the role of proteins related to actin dynamics during invasion by amastigotes remains to be investigated. Here we describe the role of Annexin A2 and ARF-6 during extracellular amastigote-mammalian cell interactions. FINDINGS: Our results showed ARF-6 accumulation in the amastigote-containing parasitophorous vacuole containing amastigote forms; demonstrated ARF-6 and Annexin A2 critical impact over parasite cell invasion and revealed the effect of Annexin A2 expression on intracellular parasite multiplication. CONCLUSION: ARF-6 and Annexin A2 are involved in invasion of mammalian cells by T. cruzi amastigotes. BioMed Central 2015-09-29 /pmc/articles/PMC4587755/ /pubmed/26416603 http://dx.doi.org/10.1186/s13071-015-1097-6 Text en © Teixeira et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Report
Teixeira, Thaise Lara
Cruz, Lilian
Mortara, Renato Arruda
Da Silva, Claudio Vieira
Revealing Annexin A2 and ARF-6 enrollment during Trypanosoma cruzi extracellular amastigote-host cell interaction
title Revealing Annexin A2 and ARF-6 enrollment during Trypanosoma cruzi extracellular amastigote-host cell interaction
title_full Revealing Annexin A2 and ARF-6 enrollment during Trypanosoma cruzi extracellular amastigote-host cell interaction
title_fullStr Revealing Annexin A2 and ARF-6 enrollment during Trypanosoma cruzi extracellular amastigote-host cell interaction
title_full_unstemmed Revealing Annexin A2 and ARF-6 enrollment during Trypanosoma cruzi extracellular amastigote-host cell interaction
title_short Revealing Annexin A2 and ARF-6 enrollment during Trypanosoma cruzi extracellular amastigote-host cell interaction
title_sort revealing annexin a2 and arf-6 enrollment during trypanosoma cruzi extracellular amastigote-host cell interaction
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4587755/
https://www.ncbi.nlm.nih.gov/pubmed/26416603
http://dx.doi.org/10.1186/s13071-015-1097-6
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