Osteogenic potential of mesenchymal cells derived from canine umbilical cord matrix co-cultured with platelet-rich plasma and demineralized bone matrix

Canine mesenchymal cells (MSCs) derived from Wharton's jelly were co-cultured, then supplemented or not supplemented with platelet rich plasma (PRP) and demineralized bone matrix (DBM) to verify osteogenic differentiation. Osteoblastic differentiation followed by mineralized bone matrix product...

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Autores principales: Souza, Talita F.B., Sakamoto, Silmara S., Ferreira, Gabriel T.N.M., Gameiro, Roberto, Marinho, Marcia, de Andrade, Alexandre L., Cardoso, Tereza C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Veterinary Science 2015
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4588025/
https://www.ncbi.nlm.nih.gov/pubmed/26040617
http://dx.doi.org/10.4142/jvs.2015.16.3.381
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author Souza, Talita F.B.
Sakamoto, Silmara S.
Ferreira, Gabriel T.N.M.
Gameiro, Roberto
Marinho, Marcia
de Andrade, Alexandre L.
Cardoso, Tereza C.
author_facet Souza, Talita F.B.
Sakamoto, Silmara S.
Ferreira, Gabriel T.N.M.
Gameiro, Roberto
Marinho, Marcia
de Andrade, Alexandre L.
Cardoso, Tereza C.
author_sort Souza, Talita F.B.
collection PubMed
description Canine mesenchymal cells (MSCs) derived from Wharton's jelly were co-cultured, then supplemented or not supplemented with platelet rich plasma (PRP) and demineralized bone matrix (DBM) to verify osteogenic differentiation. Osteoblastic differentiation followed by mineralized bone matrix production was found to be significantly higher (p < 0.05) when MSCs were associated with PRP/DBM in culture after 14-21-days of induction. Osteopontin and osteocalcin gene expression were significantly superior (p < 0.05) under the same culture conditions after 21 days of observation. In conclusion, addition of PRP to DBM co-cultured with MSCs successfully induced osteogenesis in vitro.
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spelling pubmed-45880252015-09-30 Osteogenic potential of mesenchymal cells derived from canine umbilical cord matrix co-cultured with platelet-rich plasma and demineralized bone matrix Souza, Talita F.B. Sakamoto, Silmara S. Ferreira, Gabriel T.N.M. Gameiro, Roberto Marinho, Marcia de Andrade, Alexandre L. Cardoso, Tereza C. J Vet Sci Short Communication Canine mesenchymal cells (MSCs) derived from Wharton's jelly were co-cultured, then supplemented or not supplemented with platelet rich plasma (PRP) and demineralized bone matrix (DBM) to verify osteogenic differentiation. Osteoblastic differentiation followed by mineralized bone matrix production was found to be significantly higher (p < 0.05) when MSCs were associated with PRP/DBM in culture after 14-21-days of induction. Osteopontin and osteocalcin gene expression were significantly superior (p < 0.05) under the same culture conditions after 21 days of observation. In conclusion, addition of PRP to DBM co-cultured with MSCs successfully induced osteogenesis in vitro. The Korean Society of Veterinary Science 2015-09 2015-09-21 /pmc/articles/PMC4588025/ /pubmed/26040617 http://dx.doi.org/10.4142/jvs.2015.16.3.381 Text en © 2015 The Korean Society of Veterinary Science. http://creativecommons.org/licenses/by-nc/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Souza, Talita F.B.
Sakamoto, Silmara S.
Ferreira, Gabriel T.N.M.
Gameiro, Roberto
Marinho, Marcia
de Andrade, Alexandre L.
Cardoso, Tereza C.
Osteogenic potential of mesenchymal cells derived from canine umbilical cord matrix co-cultured with platelet-rich plasma and demineralized bone matrix
title Osteogenic potential of mesenchymal cells derived from canine umbilical cord matrix co-cultured with platelet-rich plasma and demineralized bone matrix
title_full Osteogenic potential of mesenchymal cells derived from canine umbilical cord matrix co-cultured with platelet-rich plasma and demineralized bone matrix
title_fullStr Osteogenic potential of mesenchymal cells derived from canine umbilical cord matrix co-cultured with platelet-rich plasma and demineralized bone matrix
title_full_unstemmed Osteogenic potential of mesenchymal cells derived from canine umbilical cord matrix co-cultured with platelet-rich plasma and demineralized bone matrix
title_short Osteogenic potential of mesenchymal cells derived from canine umbilical cord matrix co-cultured with platelet-rich plasma and demineralized bone matrix
title_sort osteogenic potential of mesenchymal cells derived from canine umbilical cord matrix co-cultured with platelet-rich plasma and demineralized bone matrix
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4588025/
https://www.ncbi.nlm.nih.gov/pubmed/26040617
http://dx.doi.org/10.4142/jvs.2015.16.3.381
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