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Antifungal Activity of 14-Helical β-Peptides against Planktonic Cells and Biofilms of Candida Species

Candida albicans is the most prevalent cause of fungal infections and treatment is further complicated by the formation of drug resistant biofilms, often on the surfaces of implanted medical devices. In recent years, the incidence of fungal infections by other pathogenic Candida species such as C. g...

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Autores principales: Raman, Namrata, Lee, Myung-Ryul, Lynn, David M., Palecek, Sean P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4588179/
https://www.ncbi.nlm.nih.gov/pubmed/26287212
http://dx.doi.org/10.3390/ph8030483
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author Raman, Namrata
Lee, Myung-Ryul
Lynn, David M.
Palecek, Sean P.
author_facet Raman, Namrata
Lee, Myung-Ryul
Lynn, David M.
Palecek, Sean P.
author_sort Raman, Namrata
collection PubMed
description Candida albicans is the most prevalent cause of fungal infections and treatment is further complicated by the formation of drug resistant biofilms, often on the surfaces of implanted medical devices. In recent years, the incidence of fungal infections by other pathogenic Candida species such as C. glabrata, C. parapsilosis and C. tropicalis has increased. Amphiphilic, helical β-peptide structural mimetics of natural antimicrobial α-peptides have been shown to exhibit specific planktonic antifungal and anti-biofilm formation activity against C. albicans in vitro. Here, we demonstrate that β-peptides are also active against clinically isolated and drug resistant strains of C. albicans and against other opportunistic Candida spp. Different Candida species were susceptible to β-peptides to varying degrees, with C. tropicalis being the most and C. glabrata being the least susceptible. β-peptide hydrophobicity directly correlated with antifungal activity against all the Candida clinical strains and species tested. While β-peptides were largely ineffective at disrupting existing Candida biofilms, hydrophobic β-peptides were able to prevent the formation of C. albicans, C. glabrata, C. parapsilosis and C. tropicalis biofilms. The broad-spectrum antifungal activity of β-peptides against planktonic cells and in preventing biofilm formation suggests the promise of this class of molecules as therapeutics.
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spelling pubmed-45881792015-10-08 Antifungal Activity of 14-Helical β-Peptides against Planktonic Cells and Biofilms of Candida Species Raman, Namrata Lee, Myung-Ryul Lynn, David M. Palecek, Sean P. Pharmaceuticals (Basel) Article Candida albicans is the most prevalent cause of fungal infections and treatment is further complicated by the formation of drug resistant biofilms, often on the surfaces of implanted medical devices. In recent years, the incidence of fungal infections by other pathogenic Candida species such as C. glabrata, C. parapsilosis and C. tropicalis has increased. Amphiphilic, helical β-peptide structural mimetics of natural antimicrobial α-peptides have been shown to exhibit specific planktonic antifungal and anti-biofilm formation activity against C. albicans in vitro. Here, we demonstrate that β-peptides are also active against clinically isolated and drug resistant strains of C. albicans and against other opportunistic Candida spp. Different Candida species were susceptible to β-peptides to varying degrees, with C. tropicalis being the most and C. glabrata being the least susceptible. β-peptide hydrophobicity directly correlated with antifungal activity against all the Candida clinical strains and species tested. While β-peptides were largely ineffective at disrupting existing Candida biofilms, hydrophobic β-peptides were able to prevent the formation of C. albicans, C. glabrata, C. parapsilosis and C. tropicalis biofilms. The broad-spectrum antifungal activity of β-peptides against planktonic cells and in preventing biofilm formation suggests the promise of this class of molecules as therapeutics. MDPI 2015-08-13 /pmc/articles/PMC4588179/ /pubmed/26287212 http://dx.doi.org/10.3390/ph8030483 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Raman, Namrata
Lee, Myung-Ryul
Lynn, David M.
Palecek, Sean P.
Antifungal Activity of 14-Helical β-Peptides against Planktonic Cells and Biofilms of Candida Species
title Antifungal Activity of 14-Helical β-Peptides against Planktonic Cells and Biofilms of Candida Species
title_full Antifungal Activity of 14-Helical β-Peptides against Planktonic Cells and Biofilms of Candida Species
title_fullStr Antifungal Activity of 14-Helical β-Peptides against Planktonic Cells and Biofilms of Candida Species
title_full_unstemmed Antifungal Activity of 14-Helical β-Peptides against Planktonic Cells and Biofilms of Candida Species
title_short Antifungal Activity of 14-Helical β-Peptides against Planktonic Cells and Biofilms of Candida Species
title_sort antifungal activity of 14-helical β-peptides against planktonic cells and biofilms of candida species
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4588179/
https://www.ncbi.nlm.nih.gov/pubmed/26287212
http://dx.doi.org/10.3390/ph8030483
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