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Cultivated Oral Mucosa Epithelium in Ocular Surface Reconstruction in Aniridia Patients

Purpose. Efficacy of cultivated oral mucosa epithelial transplantation (COMET) procedure in corneal epithelium restoration of aniridia patients. Methods. Study subjects were aniridia patients (13 patients; 17 eyes) with irregular, vascular conjunctival pannus involving visual axis who underwent auto...

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Autores principales: Dobrowolski, Dariusz, Orzechowska-Wylegala, Boguslawa, Wowra, Bogumil, Wroblewska-Czajka, Ewa, Grolik, Maria, Szczubialka, Krzysztof, Nowakowska, Maria, Puzzolo, Domenico, Wylegala, Edward A., Micali, Antonio, Aragona, Pasquale
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4588336/
https://www.ncbi.nlm.nih.gov/pubmed/26451366
http://dx.doi.org/10.1155/2015/281870
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author Dobrowolski, Dariusz
Orzechowska-Wylegala, Boguslawa
Wowra, Bogumil
Wroblewska-Czajka, Ewa
Grolik, Maria
Szczubialka, Krzysztof
Nowakowska, Maria
Puzzolo, Domenico
Wylegala, Edward A.
Micali, Antonio
Aragona, Pasquale
author_facet Dobrowolski, Dariusz
Orzechowska-Wylegala, Boguslawa
Wowra, Bogumil
Wroblewska-Czajka, Ewa
Grolik, Maria
Szczubialka, Krzysztof
Nowakowska, Maria
Puzzolo, Domenico
Wylegala, Edward A.
Micali, Antonio
Aragona, Pasquale
author_sort Dobrowolski, Dariusz
collection PubMed
description Purpose. Efficacy of cultivated oral mucosa epithelial transplantation (COMET) procedure in corneal epithelium restoration of aniridia patients. Methods. Study subjects were aniridia patients (13 patients; 17 eyes) with irregular, vascular conjunctival pannus involving visual axis who underwent autologous transplantation of cultivated epithelium. For the procedure oral mucosa epithelial cells were obtained from buccal mucosa with further enzymatic treatment. Suspension of single cells was seeded on previously prepared denuded amniotic membrane. Cultures were carried on culture dishes inserts in the presence of the inactivated with Mitomycin C monolayer of 3T3 fibroblasts. Cultures were carried for seven days. Stratified oral mucosa epithelium with its amniotic membrane carrier was transplanted on the surgically denuded corneal surface of aniridia patients with total or subtotal limbal stem cell deficiency. Outcome Measures. Corneal surface, epithelial regularity, and visual acuity improvement were evaluated. Results. At the end of the observation period, 76.4% of the eyes had regular transparent epithelium and 23.5% had developed epithelial defects or central corneal haze; in 88.2% of cases visual acuity had increased. VA range was from HM 0.05 before the surgery to HM up to 0.1 after surgery. Conclusion. Application of cultivated oral mucosa epithelium restores regular epithelium on the corneal surface with moderate improvement in quality of vision.
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spelling pubmed-45883362015-10-08 Cultivated Oral Mucosa Epithelium in Ocular Surface Reconstruction in Aniridia Patients Dobrowolski, Dariusz Orzechowska-Wylegala, Boguslawa Wowra, Bogumil Wroblewska-Czajka, Ewa Grolik, Maria Szczubialka, Krzysztof Nowakowska, Maria Puzzolo, Domenico Wylegala, Edward A. Micali, Antonio Aragona, Pasquale Biomed Res Int Clinical Study Purpose. Efficacy of cultivated oral mucosa epithelial transplantation (COMET) procedure in corneal epithelium restoration of aniridia patients. Methods. Study subjects were aniridia patients (13 patients; 17 eyes) with irregular, vascular conjunctival pannus involving visual axis who underwent autologous transplantation of cultivated epithelium. For the procedure oral mucosa epithelial cells were obtained from buccal mucosa with further enzymatic treatment. Suspension of single cells was seeded on previously prepared denuded amniotic membrane. Cultures were carried on culture dishes inserts in the presence of the inactivated with Mitomycin C monolayer of 3T3 fibroblasts. Cultures were carried for seven days. Stratified oral mucosa epithelium with its amniotic membrane carrier was transplanted on the surgically denuded corneal surface of aniridia patients with total or subtotal limbal stem cell deficiency. Outcome Measures. Corneal surface, epithelial regularity, and visual acuity improvement were evaluated. Results. At the end of the observation period, 76.4% of the eyes had regular transparent epithelium and 23.5% had developed epithelial defects or central corneal haze; in 88.2% of cases visual acuity had increased. VA range was from HM 0.05 before the surgery to HM up to 0.1 after surgery. Conclusion. Application of cultivated oral mucosa epithelium restores regular epithelium on the corneal surface with moderate improvement in quality of vision. Hindawi Publishing Corporation 2015 2015-09-16 /pmc/articles/PMC4588336/ /pubmed/26451366 http://dx.doi.org/10.1155/2015/281870 Text en Copyright © 2015 Dariusz Dobrowolski et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Clinical Study
Dobrowolski, Dariusz
Orzechowska-Wylegala, Boguslawa
Wowra, Bogumil
Wroblewska-Czajka, Ewa
Grolik, Maria
Szczubialka, Krzysztof
Nowakowska, Maria
Puzzolo, Domenico
Wylegala, Edward A.
Micali, Antonio
Aragona, Pasquale
Cultivated Oral Mucosa Epithelium in Ocular Surface Reconstruction in Aniridia Patients
title Cultivated Oral Mucosa Epithelium in Ocular Surface Reconstruction in Aniridia Patients
title_full Cultivated Oral Mucosa Epithelium in Ocular Surface Reconstruction in Aniridia Patients
title_fullStr Cultivated Oral Mucosa Epithelium in Ocular Surface Reconstruction in Aniridia Patients
title_full_unstemmed Cultivated Oral Mucosa Epithelium in Ocular Surface Reconstruction in Aniridia Patients
title_short Cultivated Oral Mucosa Epithelium in Ocular Surface Reconstruction in Aniridia Patients
title_sort cultivated oral mucosa epithelium in ocular surface reconstruction in aniridia patients
topic Clinical Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4588336/
https://www.ncbi.nlm.nih.gov/pubmed/26451366
http://dx.doi.org/10.1155/2015/281870
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