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Cultivated Oral Mucosa Epithelium in Ocular Surface Reconstruction in Aniridia Patients
Purpose. Efficacy of cultivated oral mucosa epithelial transplantation (COMET) procedure in corneal epithelium restoration of aniridia patients. Methods. Study subjects were aniridia patients (13 patients; 17 eyes) with irregular, vascular conjunctival pannus involving visual axis who underwent auto...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4588336/ https://www.ncbi.nlm.nih.gov/pubmed/26451366 http://dx.doi.org/10.1155/2015/281870 |
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author | Dobrowolski, Dariusz Orzechowska-Wylegala, Boguslawa Wowra, Bogumil Wroblewska-Czajka, Ewa Grolik, Maria Szczubialka, Krzysztof Nowakowska, Maria Puzzolo, Domenico Wylegala, Edward A. Micali, Antonio Aragona, Pasquale |
author_facet | Dobrowolski, Dariusz Orzechowska-Wylegala, Boguslawa Wowra, Bogumil Wroblewska-Czajka, Ewa Grolik, Maria Szczubialka, Krzysztof Nowakowska, Maria Puzzolo, Domenico Wylegala, Edward A. Micali, Antonio Aragona, Pasquale |
author_sort | Dobrowolski, Dariusz |
collection | PubMed |
description | Purpose. Efficacy of cultivated oral mucosa epithelial transplantation (COMET) procedure in corneal epithelium restoration of aniridia patients. Methods. Study subjects were aniridia patients (13 patients; 17 eyes) with irregular, vascular conjunctival pannus involving visual axis who underwent autologous transplantation of cultivated epithelium. For the procedure oral mucosa epithelial cells were obtained from buccal mucosa with further enzymatic treatment. Suspension of single cells was seeded on previously prepared denuded amniotic membrane. Cultures were carried on culture dishes inserts in the presence of the inactivated with Mitomycin C monolayer of 3T3 fibroblasts. Cultures were carried for seven days. Stratified oral mucosa epithelium with its amniotic membrane carrier was transplanted on the surgically denuded corneal surface of aniridia patients with total or subtotal limbal stem cell deficiency. Outcome Measures. Corneal surface, epithelial regularity, and visual acuity improvement were evaluated. Results. At the end of the observation period, 76.4% of the eyes had regular transparent epithelium and 23.5% had developed epithelial defects or central corneal haze; in 88.2% of cases visual acuity had increased. VA range was from HM 0.05 before the surgery to HM up to 0.1 after surgery. Conclusion. Application of cultivated oral mucosa epithelium restores regular epithelium on the corneal surface with moderate improvement in quality of vision. |
format | Online Article Text |
id | pubmed-4588336 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-45883362015-10-08 Cultivated Oral Mucosa Epithelium in Ocular Surface Reconstruction in Aniridia Patients Dobrowolski, Dariusz Orzechowska-Wylegala, Boguslawa Wowra, Bogumil Wroblewska-Czajka, Ewa Grolik, Maria Szczubialka, Krzysztof Nowakowska, Maria Puzzolo, Domenico Wylegala, Edward A. Micali, Antonio Aragona, Pasquale Biomed Res Int Clinical Study Purpose. Efficacy of cultivated oral mucosa epithelial transplantation (COMET) procedure in corneal epithelium restoration of aniridia patients. Methods. Study subjects were aniridia patients (13 patients; 17 eyes) with irregular, vascular conjunctival pannus involving visual axis who underwent autologous transplantation of cultivated epithelium. For the procedure oral mucosa epithelial cells were obtained from buccal mucosa with further enzymatic treatment. Suspension of single cells was seeded on previously prepared denuded amniotic membrane. Cultures were carried on culture dishes inserts in the presence of the inactivated with Mitomycin C monolayer of 3T3 fibroblasts. Cultures were carried for seven days. Stratified oral mucosa epithelium with its amniotic membrane carrier was transplanted on the surgically denuded corneal surface of aniridia patients with total or subtotal limbal stem cell deficiency. Outcome Measures. Corneal surface, epithelial regularity, and visual acuity improvement were evaluated. Results. At the end of the observation period, 76.4% of the eyes had regular transparent epithelium and 23.5% had developed epithelial defects or central corneal haze; in 88.2% of cases visual acuity had increased. VA range was from HM 0.05 before the surgery to HM up to 0.1 after surgery. Conclusion. Application of cultivated oral mucosa epithelium restores regular epithelium on the corneal surface with moderate improvement in quality of vision. Hindawi Publishing Corporation 2015 2015-09-16 /pmc/articles/PMC4588336/ /pubmed/26451366 http://dx.doi.org/10.1155/2015/281870 Text en Copyright © 2015 Dariusz Dobrowolski et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Clinical Study Dobrowolski, Dariusz Orzechowska-Wylegala, Boguslawa Wowra, Bogumil Wroblewska-Czajka, Ewa Grolik, Maria Szczubialka, Krzysztof Nowakowska, Maria Puzzolo, Domenico Wylegala, Edward A. Micali, Antonio Aragona, Pasquale Cultivated Oral Mucosa Epithelium in Ocular Surface Reconstruction in Aniridia Patients |
title | Cultivated Oral Mucosa Epithelium in Ocular Surface Reconstruction in Aniridia Patients |
title_full | Cultivated Oral Mucosa Epithelium in Ocular Surface Reconstruction in Aniridia Patients |
title_fullStr | Cultivated Oral Mucosa Epithelium in Ocular Surface Reconstruction in Aniridia Patients |
title_full_unstemmed | Cultivated Oral Mucosa Epithelium in Ocular Surface Reconstruction in Aniridia Patients |
title_short | Cultivated Oral Mucosa Epithelium in Ocular Surface Reconstruction in Aniridia Patients |
title_sort | cultivated oral mucosa epithelium in ocular surface reconstruction in aniridia patients |
topic | Clinical Study |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4588336/ https://www.ncbi.nlm.nih.gov/pubmed/26451366 http://dx.doi.org/10.1155/2015/281870 |
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