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Determination of Early and Late Endothelial Progenitor Cells in Peripheral Circulation and Their Clinical Association with Coronary Artery Disease
The clinical implications of early and late endothelial progenitor cells (EPCs) in coronary artery disease (CAD) remain unclear. We investigated endothelial dysfunction in CAD by simultaneously examining early and late EPC colony formation and gene expression of specific surface markers in EPCs. EPC...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4588339/ https://www.ncbi.nlm.nih.gov/pubmed/26451256 http://dx.doi.org/10.1155/2015/674213 |
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author | Tagawa, Shotoku Nakanishi, Chiaki Mori, Masayuki Yoshimuta, Tsuyoshi Yoshida, Shohei Shimojima, Masaya Yokawa, Junichiro Kawashiri, Masa-aki Yamagishi, Masakazu Hayashi, Kenshi |
author_facet | Tagawa, Shotoku Nakanishi, Chiaki Mori, Masayuki Yoshimuta, Tsuyoshi Yoshida, Shohei Shimojima, Masaya Yokawa, Junichiro Kawashiri, Masa-aki Yamagishi, Masakazu Hayashi, Kenshi |
author_sort | Tagawa, Shotoku |
collection | PubMed |
description | The clinical implications of early and late endothelial progenitor cells (EPCs) in coronary artery disease (CAD) remain unclear. We investigated endothelial dysfunction in CAD by simultaneously examining early and late EPC colony formation and gene expression of specific surface markers in EPCs. EPCs were extracted from a total of 83 subjects with (n = 47) and without (n = 36) CAD. Early and late EPC colonies were formed from mononuclear cells extracted from peripheral blood. We found that fewer early EPC colonies were produced in the CAD group (7.2 ± 3.l/well) than those in the control group (12.4 ± 1.4/well, p < 0.05), and more late EPC colonies were produced in the CAD group (0.8 ± 0.2/well) than those in the control group (0.25 ± 0.02/well, p < 0.05). In the CAD group, the relative expression of CD31 and KDR of early and late EPCs was lower than in the control group. These results demonstrate that CAD patients could have increased late EPC density and that early and late EPCs in CAD patients exhibited immature endothelial characteristics. We suggest that changes in EPC colony count and gene expression of endothelial markers may have relation with development of CAD. |
format | Online Article Text |
id | pubmed-4588339 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-45883392015-10-08 Determination of Early and Late Endothelial Progenitor Cells in Peripheral Circulation and Their Clinical Association with Coronary Artery Disease Tagawa, Shotoku Nakanishi, Chiaki Mori, Masayuki Yoshimuta, Tsuyoshi Yoshida, Shohei Shimojima, Masaya Yokawa, Junichiro Kawashiri, Masa-aki Yamagishi, Masakazu Hayashi, Kenshi Int J Vasc Med Research Article The clinical implications of early and late endothelial progenitor cells (EPCs) in coronary artery disease (CAD) remain unclear. We investigated endothelial dysfunction in CAD by simultaneously examining early and late EPC colony formation and gene expression of specific surface markers in EPCs. EPCs were extracted from a total of 83 subjects with (n = 47) and without (n = 36) CAD. Early and late EPC colonies were formed from mononuclear cells extracted from peripheral blood. We found that fewer early EPC colonies were produced in the CAD group (7.2 ± 3.l/well) than those in the control group (12.4 ± 1.4/well, p < 0.05), and more late EPC colonies were produced in the CAD group (0.8 ± 0.2/well) than those in the control group (0.25 ± 0.02/well, p < 0.05). In the CAD group, the relative expression of CD31 and KDR of early and late EPCs was lower than in the control group. These results demonstrate that CAD patients could have increased late EPC density and that early and late EPCs in CAD patients exhibited immature endothelial characteristics. We suggest that changes in EPC colony count and gene expression of endothelial markers may have relation with development of CAD. Hindawi Publishing Corporation 2015 2015-09-16 /pmc/articles/PMC4588339/ /pubmed/26451256 http://dx.doi.org/10.1155/2015/674213 Text en Copyright © 2015 Shotoku Tagawa et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Tagawa, Shotoku Nakanishi, Chiaki Mori, Masayuki Yoshimuta, Tsuyoshi Yoshida, Shohei Shimojima, Masaya Yokawa, Junichiro Kawashiri, Masa-aki Yamagishi, Masakazu Hayashi, Kenshi Determination of Early and Late Endothelial Progenitor Cells in Peripheral Circulation and Their Clinical Association with Coronary Artery Disease |
title | Determination of Early and Late Endothelial Progenitor Cells in Peripheral Circulation and Their Clinical Association with Coronary Artery Disease |
title_full | Determination of Early and Late Endothelial Progenitor Cells in Peripheral Circulation and Their Clinical Association with Coronary Artery Disease |
title_fullStr | Determination of Early and Late Endothelial Progenitor Cells in Peripheral Circulation and Their Clinical Association with Coronary Artery Disease |
title_full_unstemmed | Determination of Early and Late Endothelial Progenitor Cells in Peripheral Circulation and Their Clinical Association with Coronary Artery Disease |
title_short | Determination of Early and Late Endothelial Progenitor Cells in Peripheral Circulation and Their Clinical Association with Coronary Artery Disease |
title_sort | determination of early and late endothelial progenitor cells in peripheral circulation and their clinical association with coronary artery disease |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4588339/ https://www.ncbi.nlm.nih.gov/pubmed/26451256 http://dx.doi.org/10.1155/2015/674213 |
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