Cargando…

Determination of Early and Late Endothelial Progenitor Cells in Peripheral Circulation and Their Clinical Association with Coronary Artery Disease

The clinical implications of early and late endothelial progenitor cells (EPCs) in coronary artery disease (CAD) remain unclear. We investigated endothelial dysfunction in CAD by simultaneously examining early and late EPC colony formation and gene expression of specific surface markers in EPCs. EPC...

Descripción completa

Detalles Bibliográficos
Autores principales: Tagawa, Shotoku, Nakanishi, Chiaki, Mori, Masayuki, Yoshimuta, Tsuyoshi, Yoshida, Shohei, Shimojima, Masaya, Yokawa, Junichiro, Kawashiri, Masa-aki, Yamagishi, Masakazu, Hayashi, Kenshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4588339/
https://www.ncbi.nlm.nih.gov/pubmed/26451256
http://dx.doi.org/10.1155/2015/674213
_version_ 1782392605121708032
author Tagawa, Shotoku
Nakanishi, Chiaki
Mori, Masayuki
Yoshimuta, Tsuyoshi
Yoshida, Shohei
Shimojima, Masaya
Yokawa, Junichiro
Kawashiri, Masa-aki
Yamagishi, Masakazu
Hayashi, Kenshi
author_facet Tagawa, Shotoku
Nakanishi, Chiaki
Mori, Masayuki
Yoshimuta, Tsuyoshi
Yoshida, Shohei
Shimojima, Masaya
Yokawa, Junichiro
Kawashiri, Masa-aki
Yamagishi, Masakazu
Hayashi, Kenshi
author_sort Tagawa, Shotoku
collection PubMed
description The clinical implications of early and late endothelial progenitor cells (EPCs) in coronary artery disease (CAD) remain unclear. We investigated endothelial dysfunction in CAD by simultaneously examining early and late EPC colony formation and gene expression of specific surface markers in EPCs. EPCs were extracted from a total of 83 subjects with (n = 47) and without (n = 36) CAD. Early and late EPC colonies were formed from mononuclear cells extracted from peripheral blood. We found that fewer early EPC colonies were produced in the CAD group (7.2 ± 3.l/well) than those in the control group (12.4 ± 1.4/well, p < 0.05), and more late EPC colonies were produced in the CAD group (0.8 ± 0.2/well) than those in the control group (0.25 ± 0.02/well, p < 0.05). In the CAD group, the relative expression of CD31 and KDR of early and late EPCs was lower than in the control group. These results demonstrate that CAD patients could have increased late EPC density and that early and late EPCs in CAD patients exhibited immature endothelial characteristics. We suggest that changes in EPC colony count and gene expression of endothelial markers may have relation with development of CAD.
format Online
Article
Text
id pubmed-4588339
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-45883392015-10-08 Determination of Early and Late Endothelial Progenitor Cells in Peripheral Circulation and Their Clinical Association with Coronary Artery Disease Tagawa, Shotoku Nakanishi, Chiaki Mori, Masayuki Yoshimuta, Tsuyoshi Yoshida, Shohei Shimojima, Masaya Yokawa, Junichiro Kawashiri, Masa-aki Yamagishi, Masakazu Hayashi, Kenshi Int J Vasc Med Research Article The clinical implications of early and late endothelial progenitor cells (EPCs) in coronary artery disease (CAD) remain unclear. We investigated endothelial dysfunction in CAD by simultaneously examining early and late EPC colony formation and gene expression of specific surface markers in EPCs. EPCs were extracted from a total of 83 subjects with (n = 47) and without (n = 36) CAD. Early and late EPC colonies were formed from mononuclear cells extracted from peripheral blood. We found that fewer early EPC colonies were produced in the CAD group (7.2 ± 3.l/well) than those in the control group (12.4 ± 1.4/well, p < 0.05), and more late EPC colonies were produced in the CAD group (0.8 ± 0.2/well) than those in the control group (0.25 ± 0.02/well, p < 0.05). In the CAD group, the relative expression of CD31 and KDR of early and late EPCs was lower than in the control group. These results demonstrate that CAD patients could have increased late EPC density and that early and late EPCs in CAD patients exhibited immature endothelial characteristics. We suggest that changes in EPC colony count and gene expression of endothelial markers may have relation with development of CAD. Hindawi Publishing Corporation 2015 2015-09-16 /pmc/articles/PMC4588339/ /pubmed/26451256 http://dx.doi.org/10.1155/2015/674213 Text en Copyright © 2015 Shotoku Tagawa et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Tagawa, Shotoku
Nakanishi, Chiaki
Mori, Masayuki
Yoshimuta, Tsuyoshi
Yoshida, Shohei
Shimojima, Masaya
Yokawa, Junichiro
Kawashiri, Masa-aki
Yamagishi, Masakazu
Hayashi, Kenshi
Determination of Early and Late Endothelial Progenitor Cells in Peripheral Circulation and Their Clinical Association with Coronary Artery Disease
title Determination of Early and Late Endothelial Progenitor Cells in Peripheral Circulation and Their Clinical Association with Coronary Artery Disease
title_full Determination of Early and Late Endothelial Progenitor Cells in Peripheral Circulation and Their Clinical Association with Coronary Artery Disease
title_fullStr Determination of Early and Late Endothelial Progenitor Cells in Peripheral Circulation and Their Clinical Association with Coronary Artery Disease
title_full_unstemmed Determination of Early and Late Endothelial Progenitor Cells in Peripheral Circulation and Their Clinical Association with Coronary Artery Disease
title_short Determination of Early and Late Endothelial Progenitor Cells in Peripheral Circulation and Their Clinical Association with Coronary Artery Disease
title_sort determination of early and late endothelial progenitor cells in peripheral circulation and their clinical association with coronary artery disease
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4588339/
https://www.ncbi.nlm.nih.gov/pubmed/26451256
http://dx.doi.org/10.1155/2015/674213
work_keys_str_mv AT tagawashotoku determinationofearlyandlateendothelialprogenitorcellsinperipheralcirculationandtheirclinicalassociationwithcoronaryarterydisease
AT nakanishichiaki determinationofearlyandlateendothelialprogenitorcellsinperipheralcirculationandtheirclinicalassociationwithcoronaryarterydisease
AT morimasayuki determinationofearlyandlateendothelialprogenitorcellsinperipheralcirculationandtheirclinicalassociationwithcoronaryarterydisease
AT yoshimutatsuyoshi determinationofearlyandlateendothelialprogenitorcellsinperipheralcirculationandtheirclinicalassociationwithcoronaryarterydisease
AT yoshidashohei determinationofearlyandlateendothelialprogenitorcellsinperipheralcirculationandtheirclinicalassociationwithcoronaryarterydisease
AT shimojimamasaya determinationofearlyandlateendothelialprogenitorcellsinperipheralcirculationandtheirclinicalassociationwithcoronaryarterydisease
AT yokawajunichiro determinationofearlyandlateendothelialprogenitorcellsinperipheralcirculationandtheirclinicalassociationwithcoronaryarterydisease
AT kawashirimasaaki determinationofearlyandlateendothelialprogenitorcellsinperipheralcirculationandtheirclinicalassociationwithcoronaryarterydisease
AT yamagishimasakazu determinationofearlyandlateendothelialprogenitorcellsinperipheralcirculationandtheirclinicalassociationwithcoronaryarterydisease
AT hayashikenshi determinationofearlyandlateendothelialprogenitorcellsinperipheralcirculationandtheirclinicalassociationwithcoronaryarterydisease