A diphtheria toxin resistance marker for in vitro and in vivo selection of stably transduced human cells

We developed a selectable marker rendering human cells resistant to Diphtheria Toxin (DT). The marker (DT(R)) consists of a primary microRNA sequence engineered to downregulate the ubiquitous DPH2 gene, a key enzyme for the biosynthesis of the DT target diphthamide. DT(R) expression in human cells i...

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Detalles Bibliográficos
Autores principales: Picco, Gabriele, Petti, Consalvo, Trusolino, Livio, Bertotti, Andrea, Medico, Enzo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4588510/
https://www.ncbi.nlm.nih.gov/pubmed/26420058
http://dx.doi.org/10.1038/srep14721

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