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Renal Protective Effect of Probucol in Rats with Contrast-Induced Nephropathy and its Underlying Mechanism
BACKGROUND: Contrast-induced nephropathy (CIN) refers to acute renal damage that occurs after the use of contrast agents. This study investigated the renal protective effect of probucol in a rat model of contrast-induced nephropathy and the mechanism of its effect. MATERIAL/METHODS: Twenty-eight Wis...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Scientific Literature, Inc.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4588667/ https://www.ncbi.nlm.nih.gov/pubmed/26408630 http://dx.doi.org/10.12659/MSM.895543 |
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author | Wang, Na Wei, Ri-bao Li, Qing-ping Yang, Xi Li, Ping Huang, Meng-jie Wang, Rui Cai, Guang-yan Chen, Xiang-mei |
author_facet | Wang, Na Wei, Ri-bao Li, Qing-ping Yang, Xi Li, Ping Huang, Meng-jie Wang, Rui Cai, Guang-yan Chen, Xiang-mei |
author_sort | Wang, Na |
collection | PubMed |
description | BACKGROUND: Contrast-induced nephropathy (CIN) refers to acute renal damage that occurs after the use of contrast agents. This study investigated the renal protective effect of probucol in a rat model of contrast-induced nephropathy and the mechanism of its effect. MATERIAL/METHODS: Twenty-eight Wistar rats were randomly divided into the control group, model group, N-acetylcysteine(NAC) group, and probucol group. We used a rat model of iopromide-induced CIN. One day prior to modeling, the rats received gavage. At 24 h after the modeling, blood biochemistry and urine protein were assessed. Malondialdehyde (MDA) and superoxide dismutase (SOD) were measured in renal tissue. Kidney sections were created for histopathological examination. RESULTS: The model group of rats showed significantly elevated levels of blood creatinine, urea nitrogen, 24-h urine protein, histopathological scores, and parameters of oxidative stress (P<0.05). Both the NAC and probucol groups demonstrated significantly lower Scr, BUN, and urine protein levels compared to the model group (P<0.05), with no significant difference between these 2 groups. The NAC group and the probucol group had significantly lower MDA and higher SOD than the model group at 24 h after modeling (P<0.05). The 8-OHdG-positive tubule of the probucol group and NAC group were significantly lower than those of the model group (p=0.046, P=0.0008), with significant difference between these 2 groups (P=0.024). CONCLUSIONS: Probucol can effectively reduce kidney damage caused by contrast agent. The underlying mechanism may be that probucol accelerates the recovery of renal function and renal pathology by reducing local renal oxidative stress. |
format | Online Article Text |
id | pubmed-4588667 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | International Scientific Literature, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-45886672015-10-07 Renal Protective Effect of Probucol in Rats with Contrast-Induced Nephropathy and its Underlying Mechanism Wang, Na Wei, Ri-bao Li, Qing-ping Yang, Xi Li, Ping Huang, Meng-jie Wang, Rui Cai, Guang-yan Chen, Xiang-mei Med Sci Monit Animal Study BACKGROUND: Contrast-induced nephropathy (CIN) refers to acute renal damage that occurs after the use of contrast agents. This study investigated the renal protective effect of probucol in a rat model of contrast-induced nephropathy and the mechanism of its effect. MATERIAL/METHODS: Twenty-eight Wistar rats were randomly divided into the control group, model group, N-acetylcysteine(NAC) group, and probucol group. We used a rat model of iopromide-induced CIN. One day prior to modeling, the rats received gavage. At 24 h after the modeling, blood biochemistry and urine protein were assessed. Malondialdehyde (MDA) and superoxide dismutase (SOD) were measured in renal tissue. Kidney sections were created for histopathological examination. RESULTS: The model group of rats showed significantly elevated levels of blood creatinine, urea nitrogen, 24-h urine protein, histopathological scores, and parameters of oxidative stress (P<0.05). Both the NAC and probucol groups demonstrated significantly lower Scr, BUN, and urine protein levels compared to the model group (P<0.05), with no significant difference between these 2 groups. The NAC group and the probucol group had significantly lower MDA and higher SOD than the model group at 24 h after modeling (P<0.05). The 8-OHdG-positive tubule of the probucol group and NAC group were significantly lower than those of the model group (p=0.046, P=0.0008), with significant difference between these 2 groups (P=0.024). CONCLUSIONS: Probucol can effectively reduce kidney damage caused by contrast agent. The underlying mechanism may be that probucol accelerates the recovery of renal function and renal pathology by reducing local renal oxidative stress. International Scientific Literature, Inc. 2015-09-26 /pmc/articles/PMC4588667/ /pubmed/26408630 http://dx.doi.org/10.12659/MSM.895543 Text en © Med Sci Monit, 2015 This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License |
spellingShingle | Animal Study Wang, Na Wei, Ri-bao Li, Qing-ping Yang, Xi Li, Ping Huang, Meng-jie Wang, Rui Cai, Guang-yan Chen, Xiang-mei Renal Protective Effect of Probucol in Rats with Contrast-Induced Nephropathy and its Underlying Mechanism |
title | Renal Protective Effect of Probucol in Rats with Contrast-Induced Nephropathy and its Underlying Mechanism |
title_full | Renal Protective Effect of Probucol in Rats with Contrast-Induced Nephropathy and its Underlying Mechanism |
title_fullStr | Renal Protective Effect of Probucol in Rats with Contrast-Induced Nephropathy and its Underlying Mechanism |
title_full_unstemmed | Renal Protective Effect of Probucol in Rats with Contrast-Induced Nephropathy and its Underlying Mechanism |
title_short | Renal Protective Effect of Probucol in Rats with Contrast-Induced Nephropathy and its Underlying Mechanism |
title_sort | renal protective effect of probucol in rats with contrast-induced nephropathy and its underlying mechanism |
topic | Animal Study |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4588667/ https://www.ncbi.nlm.nih.gov/pubmed/26408630 http://dx.doi.org/10.12659/MSM.895543 |
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