Variation in the ribosome interacting loop of the Sec61α from Giardia lamblia

The interaction between the ribosome and the endoplasmic reticulum-located Sec61 protein translocon is mediated through an arginine residue of Sec61α, which is conserved in all prokaryotic and eukaryotic orthologues characterized to date. Using in silico approaches we report that instead of arginine...

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Autores principales: Sinha, Abhishek, Ray, Atrayee, Ganguly, Sandipan, Ghosh Dastidar, Shubhra, Sarkar, Srimonti
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Discovery Notes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4588681/
https://www.ncbi.nlm.nih.gov/pubmed/26424409
http://dx.doi.org/10.1186/s13062-015-0087-0
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author Sinha, Abhishek
Ray, Atrayee
Ganguly, Sandipan
Ghosh Dastidar, Shubhra
Sarkar, Srimonti
author_facet Sinha, Abhishek
Ray, Atrayee
Ganguly, Sandipan
Ghosh Dastidar, Shubhra
Sarkar, Srimonti
author_sort Sinha, Abhishek
collection PubMed
description The interaction between the ribosome and the endoplasmic reticulum-located Sec61 protein translocon is mediated through an arginine residue of Sec61α, which is conserved in all prokaryotic and eukaryotic orthologues characterized to date. Using in silico approaches we report that instead of arginine, this ribosome-interaction function is most likely discharged by a lysine residue in the protist Giardia lamblia. This functional substitution of the R with a K in GlSec61α may have taken place to accommodate a G-rich rRNA. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13062-015-0087-0) contains supplementary material, which is available to authorized users.
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spelling pubmed-45886812015-10-01 Variation in the ribosome interacting loop of the Sec61α from Giardia lamblia Sinha, Abhishek Ray, Atrayee Ganguly, Sandipan Ghosh Dastidar, Shubhra Sarkar, Srimonti Biol Direct Discovery Notes The interaction between the ribosome and the endoplasmic reticulum-located Sec61 protein translocon is mediated through an arginine residue of Sec61α, which is conserved in all prokaryotic and eukaryotic orthologues characterized to date. Using in silico approaches we report that instead of arginine, this ribosome-interaction function is most likely discharged by a lysine residue in the protist Giardia lamblia. This functional substitution of the R with a K in GlSec61α may have taken place to accommodate a G-rich rRNA. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13062-015-0087-0) contains supplementary material, which is available to authorized users. BioMed Central 2015-09-30 /pmc/articles/PMC4588681/ /pubmed/26424409 http://dx.doi.org/10.1186/s13062-015-0087-0 Text en © Sinha et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Discovery Notes
Sinha, Abhishek
Ray, Atrayee
Ganguly, Sandipan
Ghosh Dastidar, Shubhra
Sarkar, Srimonti
Variation in the ribosome interacting loop of the Sec61α from Giardia lamblia
title Variation in the ribosome interacting loop of the Sec61α from Giardia lamblia
title_full Variation in the ribosome interacting loop of the Sec61α from Giardia lamblia
title_fullStr Variation in the ribosome interacting loop of the Sec61α from Giardia lamblia
title_full_unstemmed Variation in the ribosome interacting loop of the Sec61α from Giardia lamblia
title_short Variation in the ribosome interacting loop of the Sec61α from Giardia lamblia
title_sort variation in the ribosome interacting loop of the sec61α from giardia lamblia
topic Discovery Notes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4588681/
https://www.ncbi.nlm.nih.gov/pubmed/26424409
http://dx.doi.org/10.1186/s13062-015-0087-0
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