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Comparative studies of mitochondrial proteomics reveal an intimate protein network of male sterility in wheat (Triticum aestivum L.)

Plant male sterility has often been associated with mitochondrial dysfunction; however, the mechanism in wheat (Triticum aestivum L.) has not been elucidated. This study set out to probe the mechanism of physiological male sterility (PHYMS) induced by the chemical hybridizing agent (CHA)-SQ-1, and c...

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Autores principales: Wang, Shuping, Zhang, Gaisheng, Zhang, Yingxin, Song, Qilu, Chen, Zheng, Wang, Junsheng, Guo, Jialin, Niu, Na, Wang, Junwei, Ma, Shoucai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4588876/
https://www.ncbi.nlm.nih.gov/pubmed/26136264
http://dx.doi.org/10.1093/jxb/erv322
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author Wang, Shuping
Zhang, Gaisheng
Zhang, Yingxin
Song, Qilu
Chen, Zheng
Wang, Junsheng
Guo, Jialin
Niu, Na
Wang, Junwei
Ma, Shoucai
author_facet Wang, Shuping
Zhang, Gaisheng
Zhang, Yingxin
Song, Qilu
Chen, Zheng
Wang, Junsheng
Guo, Jialin
Niu, Na
Wang, Junwei
Ma, Shoucai
author_sort Wang, Shuping
collection PubMed
description Plant male sterility has often been associated with mitochondrial dysfunction; however, the mechanism in wheat (Triticum aestivum L.) has not been elucidated. This study set out to probe the mechanism of physiological male sterility (PHYMS) induced by the chemical hybridizing agent (CHA)-SQ-1, and cytoplasmic male sterility (CMS) of wheat at the proteomic level. A total of 71 differentially expressed mitochondrial proteins were found to be involved in pollen abortion and further identified by MALDI-TOF/TOF MS (matrix-assisted laser desorption/ionization-time of fight/time of flight mass spectrometry). These proteins were implicated in different cellular responses and metabolic processes, with obvious functional tendencies toward the tricarboxylic acid cycle, the mitochondrial electron transport chain, protein synthesis and degradation, oxidation stress, the cell division cycle, and epigenetics. Interactions between identified proteins were demonstrated by bioinformatics analysis, enabling a more complete insight into biological pathways involved in anther abortion and pollen defects. Accordingly, a mitochondria-mediated male sterility protein network in wheat is proposed; this network was further confirmed by physiological data, RT-PCR (real-time PCR), and TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling) assay. The results provide intriguing insights into the metabolic pathway of anther abortion induced by CHA-SQ-1 and also give useful clues to identify the crucial proteins of PHYMS and CMS in wheat.
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spelling pubmed-45888762015-10-01 Comparative studies of mitochondrial proteomics reveal an intimate protein network of male sterility in wheat (Triticum aestivum L.) Wang, Shuping Zhang, Gaisheng Zhang, Yingxin Song, Qilu Chen, Zheng Wang, Junsheng Guo, Jialin Niu, Na Wang, Junwei Ma, Shoucai J Exp Bot Research Paper Plant male sterility has often been associated with mitochondrial dysfunction; however, the mechanism in wheat (Triticum aestivum L.) has not been elucidated. This study set out to probe the mechanism of physiological male sterility (PHYMS) induced by the chemical hybridizing agent (CHA)-SQ-1, and cytoplasmic male sterility (CMS) of wheat at the proteomic level. A total of 71 differentially expressed mitochondrial proteins were found to be involved in pollen abortion and further identified by MALDI-TOF/TOF MS (matrix-assisted laser desorption/ionization-time of fight/time of flight mass spectrometry). These proteins were implicated in different cellular responses and metabolic processes, with obvious functional tendencies toward the tricarboxylic acid cycle, the mitochondrial electron transport chain, protein synthesis and degradation, oxidation stress, the cell division cycle, and epigenetics. Interactions between identified proteins were demonstrated by bioinformatics analysis, enabling a more complete insight into biological pathways involved in anther abortion and pollen defects. Accordingly, a mitochondria-mediated male sterility protein network in wheat is proposed; this network was further confirmed by physiological data, RT-PCR (real-time PCR), and TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling) assay. The results provide intriguing insights into the metabolic pathway of anther abortion induced by CHA-SQ-1 and also give useful clues to identify the crucial proteins of PHYMS and CMS in wheat. Oxford University Press 2015-09 2015-07-01 /pmc/articles/PMC4588876/ /pubmed/26136264 http://dx.doi.org/10.1093/jxb/erv322 Text en © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Wang, Shuping
Zhang, Gaisheng
Zhang, Yingxin
Song, Qilu
Chen, Zheng
Wang, Junsheng
Guo, Jialin
Niu, Na
Wang, Junwei
Ma, Shoucai
Comparative studies of mitochondrial proteomics reveal an intimate protein network of male sterility in wheat (Triticum aestivum L.)
title Comparative studies of mitochondrial proteomics reveal an intimate protein network of male sterility in wheat (Triticum aestivum L.)
title_full Comparative studies of mitochondrial proteomics reveal an intimate protein network of male sterility in wheat (Triticum aestivum L.)
title_fullStr Comparative studies of mitochondrial proteomics reveal an intimate protein network of male sterility in wheat (Triticum aestivum L.)
title_full_unstemmed Comparative studies of mitochondrial proteomics reveal an intimate protein network of male sterility in wheat (Triticum aestivum L.)
title_short Comparative studies of mitochondrial proteomics reveal an intimate protein network of male sterility in wheat (Triticum aestivum L.)
title_sort comparative studies of mitochondrial proteomics reveal an intimate protein network of male sterility in wheat (triticum aestivum l.)
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4588876/
https://www.ncbi.nlm.nih.gov/pubmed/26136264
http://dx.doi.org/10.1093/jxb/erv322
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