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miR-96 suppresses renal cell carcinoma invasion via downregulation of Ezrin expression

BACKGROUND: The present study examined the role of microRNA (miR)-96 in renal cell carcinoma (RCC) invasion. METHODS: The expression of miR-96 was detected by quantitative reverse transcription-polymerase chain reaction in human RCC cell lines with high (Caki-1) and low (786-O) metastatic potential....

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Detalles Bibliográficos
Autores principales: Yu, Nengwang, Fu, Shuai, Liu, Yubao, Xu, Zhonghua, Liu, Yi, Hao, Junwen, Wang, Baocheng, Zhang, Aimin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4588898/
https://www.ncbi.nlm.nih.gov/pubmed/26419932
http://dx.doi.org/10.1186/s13046-015-0224-8
Descripción
Sumario:BACKGROUND: The present study examined the role of microRNA (miR)-96 in renal cell carcinoma (RCC) invasion. METHODS: The expression of miR-96 was detected by quantitative reverse transcription-polymerase chain reaction in human RCC cell lines with high (Caki-1) and low (786-O) metastatic potential. Invasive ability and Ezrin expression were assessed in Caki-1 and 786-O cells transfected with a miR-96 mimic or inhibitor using wound healing assays, Transwell assays and western blotting. Expression of miR-96 and Ezrin was also examined in primary RCC samples from 17 patients with metastatic disease and 46 patients who maintained remission during a follow-up period of 37 months. RESULTS: miR-96 expression was significantly lower in Caki-1compared to786-O cells. The invasive ability of Caki-1 and 786-O cells increased following transfection of cells with miR-96 inhibitor, whereas it decreased following transfection with miR-96 mimic. Ezrin levels were negatively correlated with miR-96 in RCC, and inhibition of Ezrin expression suppressed the miR-96-induced change in invasive ability. The negative correlation between miR-96 and metastasis/Ezrin expression was also observed in human RCC specimens. CONCLUSIONS: These results suggest that miR-96 suppresses RCC invasion by modulating Ezrin expression.