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Deep sequencing analysis of transcriptomes in Aspergillus flavus in response to resveratrol
BACKGROUND: Resveratrol has been reported as a natural phytoalexin that inhibits infection or the growth of certain fungi including Aspergillus flavus. Our previous research revealed that aflatoxin production in A. flavus was reduced in medium with resveratrol. To understand the molecular mechanism...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4589122/ https://www.ncbi.nlm.nih.gov/pubmed/26420172 http://dx.doi.org/10.1186/s12866-015-0513-6 |
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author | Wang, Houmiao Lei, Yong Yan, Liying Cheng, Ke Dai, Xiaofeng Wan, Liyun Guo, Wei Cheng, Liangqiang Liao, Boshou |
author_facet | Wang, Houmiao Lei, Yong Yan, Liying Cheng, Ke Dai, Xiaofeng Wan, Liyun Guo, Wei Cheng, Liangqiang Liao, Boshou |
author_sort | Wang, Houmiao |
collection | PubMed |
description | BACKGROUND: Resveratrol has been reported as a natural phytoalexin that inhibits infection or the growth of certain fungi including Aspergillus flavus. Our previous research revealed that aflatoxin production in A. flavus was reduced in medium with resveratrol. To understand the molecular mechanism of the A. flavus response to resveratrol treatment, the high-throughput paired-end RNA-Seq was applied to analyze the transcriptomic profiles of A. flavus. RESULTS: In total, 366 and 87 genes of A. flavus were significantly up- and down- regulated, respectively, when the fungus was treated with resveratrol. Gene Ontology (GO) functional enrichment analysis revealed that 48 significantly differentially expressed genes were involved in 6 different terms. Most genes in the aflatoxin biosynthetic pathway genes cluster (#54) did not show a significant change when A. flavus was treated with resveratrol, but 23 of the 30 genes in the #54 cluster were down-regulated. The transcription of aflA and aflB was significantly suppressed under resveratrol treatment, resulting in an insufficient amount of the starter unit hexanoate for aflatoxin biosynthesis. In addition, resveratrol significantly increased the activity of antioxidative enzymes that destroy radicals, leading to decreased aflatoxin production. Moreover, stuA, fluG, flbC, and others genes involved in mycelial and conidial development were down-regulated, which disrupted the cell’s orderly differentiation and blocked conidia formation and mycelia development. The transcripts of laeA and veA were slightly inhibited by resveratrol, which may partly decrease aflatoxin production and depress conidia formation. CONCLUSIONS: Resveratrol can affect the expression of A. flavus genes that are related to developmental and secondary metabolic processes, resulting in decreased aflatoxin production and conidia formation and could also cause abnormal mycelia development. These results provide insight into the transcriptome of A. flavus in response to resveratrol and a new clew for further study in regulation of aflatoxin biosynthesis in A. flavus. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12866-015-0513-6) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4589122 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-45891222015-10-01 Deep sequencing analysis of transcriptomes in Aspergillus flavus in response to resveratrol Wang, Houmiao Lei, Yong Yan, Liying Cheng, Ke Dai, Xiaofeng Wan, Liyun Guo, Wei Cheng, Liangqiang Liao, Boshou BMC Microbiol Research Article BACKGROUND: Resveratrol has been reported as a natural phytoalexin that inhibits infection or the growth of certain fungi including Aspergillus flavus. Our previous research revealed that aflatoxin production in A. flavus was reduced in medium with resveratrol. To understand the molecular mechanism of the A. flavus response to resveratrol treatment, the high-throughput paired-end RNA-Seq was applied to analyze the transcriptomic profiles of A. flavus. RESULTS: In total, 366 and 87 genes of A. flavus were significantly up- and down- regulated, respectively, when the fungus was treated with resveratrol. Gene Ontology (GO) functional enrichment analysis revealed that 48 significantly differentially expressed genes were involved in 6 different terms. Most genes in the aflatoxin biosynthetic pathway genes cluster (#54) did not show a significant change when A. flavus was treated with resveratrol, but 23 of the 30 genes in the #54 cluster were down-regulated. The transcription of aflA and aflB was significantly suppressed under resveratrol treatment, resulting in an insufficient amount of the starter unit hexanoate for aflatoxin biosynthesis. In addition, resveratrol significantly increased the activity of antioxidative enzymes that destroy radicals, leading to decreased aflatoxin production. Moreover, stuA, fluG, flbC, and others genes involved in mycelial and conidial development were down-regulated, which disrupted the cell’s orderly differentiation and blocked conidia formation and mycelia development. The transcripts of laeA and veA were slightly inhibited by resveratrol, which may partly decrease aflatoxin production and depress conidia formation. CONCLUSIONS: Resveratrol can affect the expression of A. flavus genes that are related to developmental and secondary metabolic processes, resulting in decreased aflatoxin production and conidia formation and could also cause abnormal mycelia development. These results provide insight into the transcriptome of A. flavus in response to resveratrol and a new clew for further study in regulation of aflatoxin biosynthesis in A. flavus. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12866-015-0513-6) contains supplementary material, which is available to authorized users. BioMed Central 2015-09-16 /pmc/articles/PMC4589122/ /pubmed/26420172 http://dx.doi.org/10.1186/s12866-015-0513-6 Text en © Wang et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Wang, Houmiao Lei, Yong Yan, Liying Cheng, Ke Dai, Xiaofeng Wan, Liyun Guo, Wei Cheng, Liangqiang Liao, Boshou Deep sequencing analysis of transcriptomes in Aspergillus flavus in response to resveratrol |
title | Deep sequencing analysis of transcriptomes in Aspergillus flavus in response to resveratrol |
title_full | Deep sequencing analysis of transcriptomes in Aspergillus flavus in response to resveratrol |
title_fullStr | Deep sequencing analysis of transcriptomes in Aspergillus flavus in response to resveratrol |
title_full_unstemmed | Deep sequencing analysis of transcriptomes in Aspergillus flavus in response to resveratrol |
title_short | Deep sequencing analysis of transcriptomes in Aspergillus flavus in response to resveratrol |
title_sort | deep sequencing analysis of transcriptomes in aspergillus flavus in response to resveratrol |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4589122/ https://www.ncbi.nlm.nih.gov/pubmed/26420172 http://dx.doi.org/10.1186/s12866-015-0513-6 |
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