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Genome-Wide Analysis and Expression Profiling of the Phospholipase C Gene Family in Soybean (Glycine max)
Phosphatidylinositol-specific phospholipase C (PI-PLC) hydrolyses phosphatidylinositol-4,5-bisphosphate to produce diacylglycerol and inositol 1,4,5-trisphosphate. It plays an important role in plant development and abiotic stress responses. However, systematic analysis and expression profiling of t...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4589352/ https://www.ncbi.nlm.nih.gov/pubmed/26421918 http://dx.doi.org/10.1371/journal.pone.0138467 |
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author | Wang, Fawei Deng, Yu Zhou, Yonggang Dong, Jinye Chen, Huan Dong, Yuanyuan Wang, Nan Li, Xiaowei Li, Haiyan |
author_facet | Wang, Fawei Deng, Yu Zhou, Yonggang Dong, Jinye Chen, Huan Dong, Yuanyuan Wang, Nan Li, Xiaowei Li, Haiyan |
author_sort | Wang, Fawei |
collection | PubMed |
description | Phosphatidylinositol-specific phospholipase C (PI-PLC) hydrolyses phosphatidylinositol-4,5-bisphosphate to produce diacylglycerol and inositol 1,4,5-trisphosphate. It plays an important role in plant development and abiotic stress responses. However, systematic analysis and expression profiling of the phospholipase C (PLC) gene family in soybean have not been reported. In this study, 12 putative PLC genes were identified in the soybean genome. Soybean PLCs were found on chromosomes 2, 11, 14 and 18 and encoded 58.8–70.06 kD proteins. Expression pattern analysis by RT-PCR demonstrated that expression of the GmPLCs was induced by PEG, NaCl and saline-alkali treatments in roots and leaves. GmPLC transcripts accumulated specifically in roots after ABA treatment. Furthermore, GmPLC transcripts were analyzed in various tissues. The results showed that GmPLC7 was highly expressed in most tissues, whereas GmPLC12 was expressed in early pods specifically. In addition, subcellular localization analysis was carried out and confirmed that GmPLC10 was localized in the plasma membrane in Nicotiana benthamiana. Our genomic analysis of the soybean PLC family provides an insight into the regulation of abiotic stress responses and development. It also provides a solid foundation for the functional characterization of the soybean PLC gene family. |
format | Online Article Text |
id | pubmed-4589352 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-45893522015-10-02 Genome-Wide Analysis and Expression Profiling of the Phospholipase C Gene Family in Soybean (Glycine max) Wang, Fawei Deng, Yu Zhou, Yonggang Dong, Jinye Chen, Huan Dong, Yuanyuan Wang, Nan Li, Xiaowei Li, Haiyan PLoS One Research Article Phosphatidylinositol-specific phospholipase C (PI-PLC) hydrolyses phosphatidylinositol-4,5-bisphosphate to produce diacylglycerol and inositol 1,4,5-trisphosphate. It plays an important role in plant development and abiotic stress responses. However, systematic analysis and expression profiling of the phospholipase C (PLC) gene family in soybean have not been reported. In this study, 12 putative PLC genes were identified in the soybean genome. Soybean PLCs were found on chromosomes 2, 11, 14 and 18 and encoded 58.8–70.06 kD proteins. Expression pattern analysis by RT-PCR demonstrated that expression of the GmPLCs was induced by PEG, NaCl and saline-alkali treatments in roots and leaves. GmPLC transcripts accumulated specifically in roots after ABA treatment. Furthermore, GmPLC transcripts were analyzed in various tissues. The results showed that GmPLC7 was highly expressed in most tissues, whereas GmPLC12 was expressed in early pods specifically. In addition, subcellular localization analysis was carried out and confirmed that GmPLC10 was localized in the plasma membrane in Nicotiana benthamiana. Our genomic analysis of the soybean PLC family provides an insight into the regulation of abiotic stress responses and development. It also provides a solid foundation for the functional characterization of the soybean PLC gene family. Public Library of Science 2015-09-30 /pmc/articles/PMC4589352/ /pubmed/26421918 http://dx.doi.org/10.1371/journal.pone.0138467 Text en © 2015 Wang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Wang, Fawei Deng, Yu Zhou, Yonggang Dong, Jinye Chen, Huan Dong, Yuanyuan Wang, Nan Li, Xiaowei Li, Haiyan Genome-Wide Analysis and Expression Profiling of the Phospholipase C Gene Family in Soybean (Glycine max) |
title | Genome-Wide Analysis and Expression Profiling of the Phospholipase C Gene Family in Soybean (Glycine max) |
title_full | Genome-Wide Analysis and Expression Profiling of the Phospholipase C Gene Family in Soybean (Glycine max) |
title_fullStr | Genome-Wide Analysis and Expression Profiling of the Phospholipase C Gene Family in Soybean (Glycine max) |
title_full_unstemmed | Genome-Wide Analysis and Expression Profiling of the Phospholipase C Gene Family in Soybean (Glycine max) |
title_short | Genome-Wide Analysis and Expression Profiling of the Phospholipase C Gene Family in Soybean (Glycine max) |
title_sort | genome-wide analysis and expression profiling of the phospholipase c gene family in soybean (glycine max) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4589352/ https://www.ncbi.nlm.nih.gov/pubmed/26421918 http://dx.doi.org/10.1371/journal.pone.0138467 |
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