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Relating plaque morphology to respiratory syncytial virus subgroup, viral load, and disease severity in children
BACKGROUND: Viral culture plaque morphology in human cell lines are markers for growth capability and cytopathic effect, and have been used to assess viral fitness and select preattenuation candidates for live viral vaccines. We classified respiratory syncytial virus (RSV) plaque morphology and anal...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group US
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4589428/ https://www.ncbi.nlm.nih.gov/pubmed/26107392 http://dx.doi.org/10.1038/pr.2015.122 |
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author | Kim, Young-In Murphy, Ryan Majumdar, Sirshendu Harrison, Lisa G. Aitken, Jody DeVincenzo, John P. |
author_facet | Kim, Young-In Murphy, Ryan Majumdar, Sirshendu Harrison, Lisa G. Aitken, Jody DeVincenzo, John P. |
author_sort | Kim, Young-In |
collection | PubMed |
description | BACKGROUND: Viral culture plaque morphology in human cell lines are markers for growth capability and cytopathic effect, and have been used to assess viral fitness and select preattenuation candidates for live viral vaccines. We classified respiratory syncytial virus (RSV) plaque morphology and analyzed the relationship between plaque morphology as compared to subgroup, viral load and clinical severity of infection in infants and children. METHODS: We obtained respiratory secretions from 149 RSV-infected children. Plaque morphology and viral load was assessed within the first culture passage in HEp-2 cells. Viral load was measured by polymerase chain reaction (PCR), as was RSV subgroup. Disease severity was determined by hospitalization, length of stay, intensive care requirement, and respiratory failure. RESULTS: Plaque morphology varied between individual subjects; however, similar results were observed among viruses collected from upper and lower respiratory tracts of the same subject. Significant differences in plaque morphology were observed between RSV subgroups. No correlations were found among plaque morphology and viral load. Plaque morphology did not correlate with disease severity. CONCLUSION: Plaque morphology measures parameters that are viral-specific and independent of the human host. Morphologies vary between patients and are related to RSV subgroup. In HEp-2 cells, RSV plaque morphology appears unrelated to disease severity in RSV-infected children. SUPPLEMENTARY INFORMATION: The online version of this article (doi:10.1038/pr.2015.122) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4589428 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group US |
record_format | MEDLINE/PubMed |
spelling | pubmed-45894282016-04-01 Relating plaque morphology to respiratory syncytial virus subgroup, viral load, and disease severity in children Kim, Young-In Murphy, Ryan Majumdar, Sirshendu Harrison, Lisa G. Aitken, Jody DeVincenzo, John P. Pediatr Res Article BACKGROUND: Viral culture plaque morphology in human cell lines are markers for growth capability and cytopathic effect, and have been used to assess viral fitness and select preattenuation candidates for live viral vaccines. We classified respiratory syncytial virus (RSV) plaque morphology and analyzed the relationship between plaque morphology as compared to subgroup, viral load and clinical severity of infection in infants and children. METHODS: We obtained respiratory secretions from 149 RSV-infected children. Plaque morphology and viral load was assessed within the first culture passage in HEp-2 cells. Viral load was measured by polymerase chain reaction (PCR), as was RSV subgroup. Disease severity was determined by hospitalization, length of stay, intensive care requirement, and respiratory failure. RESULTS: Plaque morphology varied between individual subjects; however, similar results were observed among viruses collected from upper and lower respiratory tracts of the same subject. Significant differences in plaque morphology were observed between RSV subgroups. No correlations were found among plaque morphology and viral load. Plaque morphology did not correlate with disease severity. CONCLUSION: Plaque morphology measures parameters that are viral-specific and independent of the human host. Morphologies vary between patients and are related to RSV subgroup. In HEp-2 cells, RSV plaque morphology appears unrelated to disease severity in RSV-infected children. SUPPLEMENTARY INFORMATION: The online version of this article (doi:10.1038/pr.2015.122) contains supplementary material, which is available to authorized users. Nature Publishing Group US 2015-06-24 2015 /pmc/articles/PMC4589428/ /pubmed/26107392 http://dx.doi.org/10.1038/pr.2015.122 Text en © International Pediatric Research Foundation, Inc. 2015 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Article Kim, Young-In Murphy, Ryan Majumdar, Sirshendu Harrison, Lisa G. Aitken, Jody DeVincenzo, John P. Relating plaque morphology to respiratory syncytial virus subgroup, viral load, and disease severity in children |
title | Relating plaque morphology to respiratory syncytial virus subgroup, viral load, and disease severity in children |
title_full | Relating plaque morphology to respiratory syncytial virus subgroup, viral load, and disease severity in children |
title_fullStr | Relating plaque morphology to respiratory syncytial virus subgroup, viral load, and disease severity in children |
title_full_unstemmed | Relating plaque morphology to respiratory syncytial virus subgroup, viral load, and disease severity in children |
title_short | Relating plaque morphology to respiratory syncytial virus subgroup, viral load, and disease severity in children |
title_sort | relating plaque morphology to respiratory syncytial virus subgroup, viral load, and disease severity in children |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4589428/ https://www.ncbi.nlm.nih.gov/pubmed/26107392 http://dx.doi.org/10.1038/pr.2015.122 |
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