Continuous directed evolution of DNA-binding proteins to improve TALEN specificity

Nucleases containing programmable DNA-binding domains can alter the genomes of model organisms and have the potential to become human therapeutics. Here we present DNA-binding phage-assisted continuous evolution (DB-PACE) as a general approach for the laboratory evolution of DNA-binding activity and...

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Autores principales: Hubbard, Basil P., Badran, Ahmed H., Zuris, John A., Guilinger, John P., Davis, Kevin M., Chen, Liwei, Tsai, Shengdar Q., Sander, Jeffry D., Joung, J. Keith, Liu, David R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4589463/
https://www.ncbi.nlm.nih.gov/pubmed/26258293
http://dx.doi.org/10.1038/nmeth.3515
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author Hubbard, Basil P.
Badran, Ahmed H.
Zuris, John A.
Guilinger, John P.
Davis, Kevin M.
Chen, Liwei
Tsai, Shengdar Q.
Sander, Jeffry D.
Joung, J. Keith
Liu, David R.
author_facet Hubbard, Basil P.
Badran, Ahmed H.
Zuris, John A.
Guilinger, John P.
Davis, Kevin M.
Chen, Liwei
Tsai, Shengdar Q.
Sander, Jeffry D.
Joung, J. Keith
Liu, David R.
author_sort Hubbard, Basil P.
collection PubMed
description Nucleases containing programmable DNA-binding domains can alter the genomes of model organisms and have the potential to become human therapeutics. Here we present DNA-binding phage-assisted continuous evolution (DB-PACE) as a general approach for the laboratory evolution of DNA-binding activity and specificity. We used this system to generate TALE nucleases with broadly improved DNA cleavage specificity, establishing DB-PACE as a versatile approach for improving the accuracy of genome-editing agents.
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spelling pubmed-45894632016-04-01 Continuous directed evolution of DNA-binding proteins to improve TALEN specificity Hubbard, Basil P. Badran, Ahmed H. Zuris, John A. Guilinger, John P. Davis, Kevin M. Chen, Liwei Tsai, Shengdar Q. Sander, Jeffry D. Joung, J. Keith Liu, David R. Nat Methods Article Nucleases containing programmable DNA-binding domains can alter the genomes of model organisms and have the potential to become human therapeutics. Here we present DNA-binding phage-assisted continuous evolution (DB-PACE) as a general approach for the laboratory evolution of DNA-binding activity and specificity. We used this system to generate TALE nucleases with broadly improved DNA cleavage specificity, establishing DB-PACE as a versatile approach for improving the accuracy of genome-editing agents. 2015-08-10 2015-10 /pmc/articles/PMC4589463/ /pubmed/26258293 http://dx.doi.org/10.1038/nmeth.3515 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Hubbard, Basil P.
Badran, Ahmed H.
Zuris, John A.
Guilinger, John P.
Davis, Kevin M.
Chen, Liwei
Tsai, Shengdar Q.
Sander, Jeffry D.
Joung, J. Keith
Liu, David R.
Continuous directed evolution of DNA-binding proteins to improve TALEN specificity
title Continuous directed evolution of DNA-binding proteins to improve TALEN specificity
title_full Continuous directed evolution of DNA-binding proteins to improve TALEN specificity
title_fullStr Continuous directed evolution of DNA-binding proteins to improve TALEN specificity
title_full_unstemmed Continuous directed evolution of DNA-binding proteins to improve TALEN specificity
title_short Continuous directed evolution of DNA-binding proteins to improve TALEN specificity
title_sort continuous directed evolution of dna-binding proteins to improve talen specificity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4589463/
https://www.ncbi.nlm.nih.gov/pubmed/26258293
http://dx.doi.org/10.1038/nmeth.3515
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