A microfluidic device for epigenomic profiling using 100 cells

The sensitivity of chromatin immunoprecipitation (ChIP) assays poses a major obstacle for epigenomic studies of low-abundance cells. Here we present a microfluidics-based ChIP-Seq protocol using as few as 100 cells via drastically improved collection of high-quality ChIP-enriched DNA. Using this tec...

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Detalles Bibliográficos
Autores principales: Cao, Zhenning, Chen, Changya, He, Bing, Tan, Kai, Lu, Chang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4589469/
https://www.ncbi.nlm.nih.gov/pubmed/26214128
http://dx.doi.org/10.1038/nmeth.3488
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author Cao, Zhenning
Chen, Changya
He, Bing
Tan, Kai
Lu, Chang
author_facet Cao, Zhenning
Chen, Changya
He, Bing
Tan, Kai
Lu, Chang
author_sort Cao, Zhenning
collection PubMed
description The sensitivity of chromatin immunoprecipitation (ChIP) assays poses a major obstacle for epigenomic studies of low-abundance cells. Here we present a microfluidics-based ChIP-Seq protocol using as few as 100 cells via drastically improved collection of high-quality ChIP-enriched DNA. Using this technology, we uncovered many novel enhancers and super enhancers in hematopoietic stem and progenitor cells from mouse fetal liver, suggesting that enhancer activity is highly dynamic during early hematopoiesis.
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spelling pubmed-45894692016-04-01 A microfluidic device for epigenomic profiling using 100 cells Cao, Zhenning Chen, Changya He, Bing Tan, Kai Lu, Chang Nat Methods Article The sensitivity of chromatin immunoprecipitation (ChIP) assays poses a major obstacle for epigenomic studies of low-abundance cells. Here we present a microfluidics-based ChIP-Seq protocol using as few as 100 cells via drastically improved collection of high-quality ChIP-enriched DNA. Using this technology, we uncovered many novel enhancers and super enhancers in hematopoietic stem and progenitor cells from mouse fetal liver, suggesting that enhancer activity is highly dynamic during early hematopoiesis. 2015-07-27 2015-10 /pmc/articles/PMC4589469/ /pubmed/26214128 http://dx.doi.org/10.1038/nmeth.3488 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Cao, Zhenning
Chen, Changya
He, Bing
Tan, Kai
Lu, Chang
A microfluidic device for epigenomic profiling using 100 cells
title A microfluidic device for epigenomic profiling using 100 cells
title_full A microfluidic device for epigenomic profiling using 100 cells
title_fullStr A microfluidic device for epigenomic profiling using 100 cells
title_full_unstemmed A microfluidic device for epigenomic profiling using 100 cells
title_short A microfluidic device for epigenomic profiling using 100 cells
title_sort microfluidic device for epigenomic profiling using 100 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4589469/
https://www.ncbi.nlm.nih.gov/pubmed/26214128
http://dx.doi.org/10.1038/nmeth.3488
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