ChIPmentation: fast, robust, low-input ChIP-seq for histones and transcription factors

Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is widely used to map histone marks and transcription factor binding throughout the genome. Here we present ChIPmentation, a method that combines chromatin immunoprecipitation with sequencing library preparation by Tn5 transposase (“tag...

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Detalles Bibliográficos
Autores principales: Schmidl, Christian, Rendeiro, André F., Sheffield, Nathan C., Bock, Christoph
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4589892/
https://www.ncbi.nlm.nih.gov/pubmed/26280331
http://dx.doi.org/10.1038/nmeth.3542
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author Schmidl, Christian
Rendeiro, André F.
Sheffield, Nathan C.
Bock, Christoph
author_facet Schmidl, Christian
Rendeiro, André F.
Sheffield, Nathan C.
Bock, Christoph
author_sort Schmidl, Christian
collection PubMed
description Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is widely used to map histone marks and transcription factor binding throughout the genome. Here we present ChIPmentation, a method that combines chromatin immunoprecipitation with sequencing library preparation by Tn5 transposase (“tagmentation”). ChIPmentation introduces sequencing-compatible adapters in a single-step reaction directly on bead-bound chromatin, which reduces time, cost, and input requirements, thus providing a convenient and broadly useful alternative to existing ChIP-seq protocols.
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spelling pubmed-45898922016-04-01 ChIPmentation: fast, robust, low-input ChIP-seq for histones and transcription factors Schmidl, Christian Rendeiro, André F. Sheffield, Nathan C. Bock, Christoph Nat Methods Article Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is widely used to map histone marks and transcription factor binding throughout the genome. Here we present ChIPmentation, a method that combines chromatin immunoprecipitation with sequencing library preparation by Tn5 transposase (“tagmentation”). ChIPmentation introduces sequencing-compatible adapters in a single-step reaction directly on bead-bound chromatin, which reduces time, cost, and input requirements, thus providing a convenient and broadly useful alternative to existing ChIP-seq protocols. 2015-08-17 2015-10 /pmc/articles/PMC4589892/ /pubmed/26280331 http://dx.doi.org/10.1038/nmeth.3542 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Schmidl, Christian
Rendeiro, André F.
Sheffield, Nathan C.
Bock, Christoph
ChIPmentation: fast, robust, low-input ChIP-seq for histones and transcription factors
title ChIPmentation: fast, robust, low-input ChIP-seq for histones and transcription factors
title_full ChIPmentation: fast, robust, low-input ChIP-seq for histones and transcription factors
title_fullStr ChIPmentation: fast, robust, low-input ChIP-seq for histones and transcription factors
title_full_unstemmed ChIPmentation: fast, robust, low-input ChIP-seq for histones and transcription factors
title_short ChIPmentation: fast, robust, low-input ChIP-seq for histones and transcription factors
title_sort chipmentation: fast, robust, low-input chip-seq for histones and transcription factors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4589892/
https://www.ncbi.nlm.nih.gov/pubmed/26280331
http://dx.doi.org/10.1038/nmeth.3542
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