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Evidence of cAMP involvement in cellobiohydrolase expression and secretion by Trichoderma reesei in presence of the inducer sophorose

BACKGROUND: The signaling second messenger cyclic AMP (cAMP) regulates many aspects of cellular function in all organisms. Previous studies have suggested a role for cAMP in the regulation of gene expression of cellulolytic enzymes in Trichoderma reesei (anamorph of Hypocrea jecorina). METHODS: The...

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Autores principales: Nogueira, Karoline Maria Vieira, Costa, Mariana do Nascimento, de Paula, Renato Graciano, Mendonça-Natividade, Flávia Costa, Ricci-Azevedo, Rafael, Silva, Roberto Nascimento
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4590280/
https://www.ncbi.nlm.nih.gov/pubmed/26424592
http://dx.doi.org/10.1186/s12866-015-0536-z
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author Nogueira, Karoline Maria Vieira
Costa, Mariana do Nascimento
de Paula, Renato Graciano
Mendonça-Natividade, Flávia Costa
Ricci-Azevedo, Rafael
Silva, Roberto Nascimento
author_facet Nogueira, Karoline Maria Vieira
Costa, Mariana do Nascimento
de Paula, Renato Graciano
Mendonça-Natividade, Flávia Costa
Ricci-Azevedo, Rafael
Silva, Roberto Nascimento
author_sort Nogueira, Karoline Maria Vieira
collection PubMed
description BACKGROUND: The signaling second messenger cyclic AMP (cAMP) regulates many aspects of cellular function in all organisms. Previous studies have suggested a role for cAMP in the regulation of gene expression of cellulolytic enzymes in Trichoderma reesei (anamorph of Hypocrea jecorina). METHODS: The effects of cAMP in T. reesei were analyzed through both activity and expression of cellulase, intracellular cAMP level measurement, western blotting, indirect immunofluorescence and confocal microscopy. RESULTS: To elucidate the involvement of cAMP in the cellulase expression, we analyzed the growth of the mutant strain ∆acy1 and its parental strain QM9414 in the presence of the inducers cellulose, cellobiose, lactose, or sophorose, and the repressor glucose. Our results indicated that cAMP regulates the expression of cellulase in a carbon source-dependent manner. The expression cel7a, and cel6a genes was higher in the presence of sophorose than in the presence of cellulose, lactose, cellobiose, or glucose. Moreover, intracellular levels of cAMP were up to four times higher in the presence of sophorose compared to other carbon sources. Concomitantly, our immunofluorescence microscopy and western blot data suggest that in the presence of sophorose, cAMP may regulate secretion of cellulolytic enzymes in T. reesei. CONCLUSIONS: These results allow us to better understand the role of cAMP and expand our knowledge on the signal transduction pathways involved in the regulation of cellulase expression in T. reesei. Finally, our data may help develop new strategies to improve the expression of cel7a and cel6a genes, and therefore, favor their application in several biotechnology fields. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12866-015-0536-z) contains supplementary material, which is available to authorized users.
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spelling pubmed-45902802015-10-02 Evidence of cAMP involvement in cellobiohydrolase expression and secretion by Trichoderma reesei in presence of the inducer sophorose Nogueira, Karoline Maria Vieira Costa, Mariana do Nascimento de Paula, Renato Graciano Mendonça-Natividade, Flávia Costa Ricci-Azevedo, Rafael Silva, Roberto Nascimento BMC Microbiol Research Article BACKGROUND: The signaling second messenger cyclic AMP (cAMP) regulates many aspects of cellular function in all organisms. Previous studies have suggested a role for cAMP in the regulation of gene expression of cellulolytic enzymes in Trichoderma reesei (anamorph of Hypocrea jecorina). METHODS: The effects of cAMP in T. reesei were analyzed through both activity and expression of cellulase, intracellular cAMP level measurement, western blotting, indirect immunofluorescence and confocal microscopy. RESULTS: To elucidate the involvement of cAMP in the cellulase expression, we analyzed the growth of the mutant strain ∆acy1 and its parental strain QM9414 in the presence of the inducers cellulose, cellobiose, lactose, or sophorose, and the repressor glucose. Our results indicated that cAMP regulates the expression of cellulase in a carbon source-dependent manner. The expression cel7a, and cel6a genes was higher in the presence of sophorose than in the presence of cellulose, lactose, cellobiose, or glucose. Moreover, intracellular levels of cAMP were up to four times higher in the presence of sophorose compared to other carbon sources. Concomitantly, our immunofluorescence microscopy and western blot data suggest that in the presence of sophorose, cAMP may regulate secretion of cellulolytic enzymes in T. reesei. CONCLUSIONS: These results allow us to better understand the role of cAMP and expand our knowledge on the signal transduction pathways involved in the regulation of cellulase expression in T. reesei. Finally, our data may help develop new strategies to improve the expression of cel7a and cel6a genes, and therefore, favor their application in several biotechnology fields. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12866-015-0536-z) contains supplementary material, which is available to authorized users. BioMed Central 2015-09-30 /pmc/articles/PMC4590280/ /pubmed/26424592 http://dx.doi.org/10.1186/s12866-015-0536-z Text en © Nogueira et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Nogueira, Karoline Maria Vieira
Costa, Mariana do Nascimento
de Paula, Renato Graciano
Mendonça-Natividade, Flávia Costa
Ricci-Azevedo, Rafael
Silva, Roberto Nascimento
Evidence of cAMP involvement in cellobiohydrolase expression and secretion by Trichoderma reesei in presence of the inducer sophorose
title Evidence of cAMP involvement in cellobiohydrolase expression and secretion by Trichoderma reesei in presence of the inducer sophorose
title_full Evidence of cAMP involvement in cellobiohydrolase expression and secretion by Trichoderma reesei in presence of the inducer sophorose
title_fullStr Evidence of cAMP involvement in cellobiohydrolase expression and secretion by Trichoderma reesei in presence of the inducer sophorose
title_full_unstemmed Evidence of cAMP involvement in cellobiohydrolase expression and secretion by Trichoderma reesei in presence of the inducer sophorose
title_short Evidence of cAMP involvement in cellobiohydrolase expression and secretion by Trichoderma reesei in presence of the inducer sophorose
title_sort evidence of camp involvement in cellobiohydrolase expression and secretion by trichoderma reesei in presence of the inducer sophorose
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4590280/
https://www.ncbi.nlm.nih.gov/pubmed/26424592
http://dx.doi.org/10.1186/s12866-015-0536-z
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