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Enhancing production of ergosterol in Pichia pastoris GS115 by over-expression of 3-hydroxy-3-methylglutaryl CoA reductase from Glycyrrhiza uralensis

The rate-limiting enzyme in the mevalonic acid (MVA) pathway which can lead to triterpenoid saponin glycyrrhizic acid (GA) is 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR). In order to reveal the effect of copy number variation in the HMGR gene on the MVA pathway, the HMGR gene from Glycyrrhiza ur...

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Autores principales: Liu, Ying, Zhu, Xiaoqing, Li, Wendong, Wen, Hao, Gao, Ya, Liu, Yong, Liu, Chunsheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4590296/
https://www.ncbi.nlm.nih.gov/pubmed/26579379
http://dx.doi.org/10.1016/j.apsb.2014.02.007
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author Liu, Ying
Zhu, Xiaoqing
Li, Wendong
Wen, Hao
Gao, Ya
Liu, Yong
Liu, Chunsheng
author_facet Liu, Ying
Zhu, Xiaoqing
Li, Wendong
Wen, Hao
Gao, Ya
Liu, Yong
Liu, Chunsheng
author_sort Liu, Ying
collection PubMed
description The rate-limiting enzyme in the mevalonic acid (MVA) pathway which can lead to triterpenoid saponin glycyrrhizic acid (GA) is 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR). In order to reveal the effect of copy number variation in the HMGR gene on the MVA pathway, the HMGR gene from Glycyrrhiza uralensis Fisch. (GuHMGR) was cloned and over-expressed in Pichia pastoris GS115. Six recombinant P. pastoris strains containing different copy numbers of the GuHMGR gene were obtained and the content of ergosterol was analyzed by HPLC. The results showed that all the recombinant P. pastoris strains contained more ergosterol than the negative control and the strains with 8 and 44 copies contained significantly more ergosterol than the other strains. However, as the copy number increased, the content of ergosterol showed an increasing–decreasing–increasing pattern. This study provides a rationale for increasing the content of GA through over-expressing the GuHMGR gene in cultivars of G. uralensis.
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spelling pubmed-45902962015-11-17 Enhancing production of ergosterol in Pichia pastoris GS115 by over-expression of 3-hydroxy-3-methylglutaryl CoA reductase from Glycyrrhiza uralensis Liu, Ying Zhu, Xiaoqing Li, Wendong Wen, Hao Gao, Ya Liu, Yong Liu, Chunsheng Acta Pharm Sin B Original Article The rate-limiting enzyme in the mevalonic acid (MVA) pathway which can lead to triterpenoid saponin glycyrrhizic acid (GA) is 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR). In order to reveal the effect of copy number variation in the HMGR gene on the MVA pathway, the HMGR gene from Glycyrrhiza uralensis Fisch. (GuHMGR) was cloned and over-expressed in Pichia pastoris GS115. Six recombinant P. pastoris strains containing different copy numbers of the GuHMGR gene were obtained and the content of ergosterol was analyzed by HPLC. The results showed that all the recombinant P. pastoris strains contained more ergosterol than the negative control and the strains with 8 and 44 copies contained significantly more ergosterol than the other strains. However, as the copy number increased, the content of ergosterol showed an increasing–decreasing–increasing pattern. This study provides a rationale for increasing the content of GA through over-expressing the GuHMGR gene in cultivars of G. uralensis. Elsevier 2014-04 2014-04-02 /pmc/articles/PMC4590296/ /pubmed/26579379 http://dx.doi.org/10.1016/j.apsb.2014.02.007 Text en © 2014 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/3.0/ Open access under CC BY-NC-ND license.(http://creativecommons.org/licenses/by-nc-nd/3.0/).
spellingShingle Original Article
Liu, Ying
Zhu, Xiaoqing
Li, Wendong
Wen, Hao
Gao, Ya
Liu, Yong
Liu, Chunsheng
Enhancing production of ergosterol in Pichia pastoris GS115 by over-expression of 3-hydroxy-3-methylglutaryl CoA reductase from Glycyrrhiza uralensis
title Enhancing production of ergosterol in Pichia pastoris GS115 by over-expression of 3-hydroxy-3-methylglutaryl CoA reductase from Glycyrrhiza uralensis
title_full Enhancing production of ergosterol in Pichia pastoris GS115 by over-expression of 3-hydroxy-3-methylglutaryl CoA reductase from Glycyrrhiza uralensis
title_fullStr Enhancing production of ergosterol in Pichia pastoris GS115 by over-expression of 3-hydroxy-3-methylglutaryl CoA reductase from Glycyrrhiza uralensis
title_full_unstemmed Enhancing production of ergosterol in Pichia pastoris GS115 by over-expression of 3-hydroxy-3-methylglutaryl CoA reductase from Glycyrrhiza uralensis
title_short Enhancing production of ergosterol in Pichia pastoris GS115 by over-expression of 3-hydroxy-3-methylglutaryl CoA reductase from Glycyrrhiza uralensis
title_sort enhancing production of ergosterol in pichia pastoris gs115 by over-expression of 3-hydroxy-3-methylglutaryl coa reductase from glycyrrhiza uralensis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4590296/
https://www.ncbi.nlm.nih.gov/pubmed/26579379
http://dx.doi.org/10.1016/j.apsb.2014.02.007
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