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Pathogens in COPD exacerbations identified by comprehensive real-time PCR plus older methods

Respiratory infection is a major cause of exacerbation in chronic obstructive pulmonary disease (COPD). Infectious contributions to exacerbations remain incompletely described. We therefore analyzed respiratory tract samples by comprehensive real-time polymerase chain reaction (PCR) in combination w...

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Autores principales: Shimizu, Kenichiro, Yoshii, Yutaka, Morozumi, Miyuki, Chiba, Naoko, Ubukata, Kimiko, Uruga, Hironori, Hanada, Shigeo, Saito, Nayuta, Kadota, Tsukasa, Ito, Saburo, Wakui, Hiroshi, Takasaka, Naoki, Minagawa, Shunsuke, Kojima, Jun, Hara, Hiromichi, Numata, Takanori, Kawaishi, Makoto, Saito, Keisuke, Araya, Jun, Kaneko, Yumi, Nakayama, Katsutoshi, Kishi, Kazuma, Kuwano, Kazuyoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4590318/
https://www.ncbi.nlm.nih.gov/pubmed/26451098
http://dx.doi.org/10.2147/COPD.S82752
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author Shimizu, Kenichiro
Yoshii, Yutaka
Morozumi, Miyuki
Chiba, Naoko
Ubukata, Kimiko
Uruga, Hironori
Hanada, Shigeo
Saito, Nayuta
Kadota, Tsukasa
Ito, Saburo
Wakui, Hiroshi
Takasaka, Naoki
Minagawa, Shunsuke
Kojima, Jun
Hara, Hiromichi
Numata, Takanori
Kawaishi, Makoto
Saito, Keisuke
Araya, Jun
Kaneko, Yumi
Nakayama, Katsutoshi
Kishi, Kazuma
Kuwano, Kazuyoshi
author_facet Shimizu, Kenichiro
Yoshii, Yutaka
Morozumi, Miyuki
Chiba, Naoko
Ubukata, Kimiko
Uruga, Hironori
Hanada, Shigeo
Saito, Nayuta
Kadota, Tsukasa
Ito, Saburo
Wakui, Hiroshi
Takasaka, Naoki
Minagawa, Shunsuke
Kojima, Jun
Hara, Hiromichi
Numata, Takanori
Kawaishi, Makoto
Saito, Keisuke
Araya, Jun
Kaneko, Yumi
Nakayama, Katsutoshi
Kishi, Kazuma
Kuwano, Kazuyoshi
author_sort Shimizu, Kenichiro
collection PubMed
description Respiratory infection is a major cause of exacerbation in chronic obstructive pulmonary disease (COPD). Infectious contributions to exacerbations remain incompletely described. We therefore analyzed respiratory tract samples by comprehensive real-time polymerase chain reaction (PCR) in combination with conventional methods. We evaluated multiple risk factors for prolonged hospitalization to manage COPD exacerbations, including infectious agents. Over 19 months, we prospectively studied 46 patients with 50 COPD exacerbations, collecting nasopharyngeal swab and sputum samples from each. We carried out real-time PCR designed to detect six bacterial species and eleven viruses, together with conventional procedures, including sputum culture. Infectious etiologies of COPD exacerbations were identified in 44 of 50 exacerbations (88%). Infections were viral in 17 of 50 exacerbations (34%). COPD exacerbations caused by Gram-negative bacilli, including enteric and nonfermenting organisms, were significantly associated with prolonged hospitalization for COPD exacerbations. Our results support the use of a combination of real-time PCR and conventional methods for determining both infectious etiologies and risk of extended hospitalization.
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spelling pubmed-45903182015-10-08 Pathogens in COPD exacerbations identified by comprehensive real-time PCR plus older methods Shimizu, Kenichiro Yoshii, Yutaka Morozumi, Miyuki Chiba, Naoko Ubukata, Kimiko Uruga, Hironori Hanada, Shigeo Saito, Nayuta Kadota, Tsukasa Ito, Saburo Wakui, Hiroshi Takasaka, Naoki Minagawa, Shunsuke Kojima, Jun Hara, Hiromichi Numata, Takanori Kawaishi, Makoto Saito, Keisuke Araya, Jun Kaneko, Yumi Nakayama, Katsutoshi Kishi, Kazuma Kuwano, Kazuyoshi Int J Chron Obstruct Pulmon Dis Original Research Respiratory infection is a major cause of exacerbation in chronic obstructive pulmonary disease (COPD). Infectious contributions to exacerbations remain incompletely described. We therefore analyzed respiratory tract samples by comprehensive real-time polymerase chain reaction (PCR) in combination with conventional methods. We evaluated multiple risk factors for prolonged hospitalization to manage COPD exacerbations, including infectious agents. Over 19 months, we prospectively studied 46 patients with 50 COPD exacerbations, collecting nasopharyngeal swab and sputum samples from each. We carried out real-time PCR designed to detect six bacterial species and eleven viruses, together with conventional procedures, including sputum culture. Infectious etiologies of COPD exacerbations were identified in 44 of 50 exacerbations (88%). Infections were viral in 17 of 50 exacerbations (34%). COPD exacerbations caused by Gram-negative bacilli, including enteric and nonfermenting organisms, were significantly associated with prolonged hospitalization for COPD exacerbations. Our results support the use of a combination of real-time PCR and conventional methods for determining both infectious etiologies and risk of extended hospitalization. Dove Medical Press 2015-09-23 /pmc/articles/PMC4590318/ /pubmed/26451098 http://dx.doi.org/10.2147/COPD.S82752 Text en © 2015 Shimizu et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Shimizu, Kenichiro
Yoshii, Yutaka
Morozumi, Miyuki
Chiba, Naoko
Ubukata, Kimiko
Uruga, Hironori
Hanada, Shigeo
Saito, Nayuta
Kadota, Tsukasa
Ito, Saburo
Wakui, Hiroshi
Takasaka, Naoki
Minagawa, Shunsuke
Kojima, Jun
Hara, Hiromichi
Numata, Takanori
Kawaishi, Makoto
Saito, Keisuke
Araya, Jun
Kaneko, Yumi
Nakayama, Katsutoshi
Kishi, Kazuma
Kuwano, Kazuyoshi
Pathogens in COPD exacerbations identified by comprehensive real-time PCR plus older methods
title Pathogens in COPD exacerbations identified by comprehensive real-time PCR plus older methods
title_full Pathogens in COPD exacerbations identified by comprehensive real-time PCR plus older methods
title_fullStr Pathogens in COPD exacerbations identified by comprehensive real-time PCR plus older methods
title_full_unstemmed Pathogens in COPD exacerbations identified by comprehensive real-time PCR plus older methods
title_short Pathogens in COPD exacerbations identified by comprehensive real-time PCR plus older methods
title_sort pathogens in copd exacerbations identified by comprehensive real-time pcr plus older methods
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4590318/
https://www.ncbi.nlm.nih.gov/pubmed/26451098
http://dx.doi.org/10.2147/COPD.S82752
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