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Transdermal Delivery of Small Interfering RNA with Elastic Cationic Liposomes in Mice

We developed elastic cationic liposomal vectors for transdermal siRNA delivery. These liposomes were prepared with 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) as a cationic lipid and sodium cholate (NaChol) or Tween 80 as an edge activator. When NaChol or Tween 80 was included at 5, 10, and 15%...

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Detalles Bibliográficos
Autores principales: Hattori, Yoshiyuki, Date, Masataka, Arai, Shohei, Kawano, Kumi, Yonemochi, Etsuo, Maitani, Yoshie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4590792/
https://www.ncbi.nlm.nih.gov/pubmed/26555966
http://dx.doi.org/10.1155/2013/149695
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author Hattori, Yoshiyuki
Date, Masataka
Arai, Shohei
Kawano, Kumi
Yonemochi, Etsuo
Maitani, Yoshie
author_facet Hattori, Yoshiyuki
Date, Masataka
Arai, Shohei
Kawano, Kumi
Yonemochi, Etsuo
Maitani, Yoshie
author_sort Hattori, Yoshiyuki
collection PubMed
description We developed elastic cationic liposomal vectors for transdermal siRNA delivery. These liposomes were prepared with 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) as a cationic lipid and sodium cholate (NaChol) or Tween 80 as an edge activator. When NaChol or Tween 80 was included at 5, 10, and 15% (w/w) into DOTAP liposomal formulations (C5-, C10-, and C15-liposomes and T5-, T10-, and T15-liposomes), C15- and T10-liposomes showed 2.4- and 2.7-fold-higher elasticities than DOTAP liposome, respectively. Although the sizes of all elastic liposomes prepared in this study were about 80–90 nm, the sizes of C5-, C10- and C15-liposome/siRNA complexes (lipoplexes) were about 1,700–1,800 nm, and those of T5-, T10-, and T15-lipoplexes were about 550–780 nm. Their elastic lipoplexes showed strong gene suppression by siRNA without cytotoxicity when transfected into human cervical carcinoma SiHa cells. Following skin application of the fluorescence-labeled lipoplexes in mice, among the elastic lipoplexes, C15- and T5-lipoplexes showed effective penetration of siRNA into skin, compared with DOTAP lipoplex and free siRNA solution. These data suggest that elastic cationic liposomes containing an appropriate amount of NaChol or Tween 80 as an edge activator could deliver siRNA transdermally.
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spelling pubmed-45907922015-10-13 Transdermal Delivery of Small Interfering RNA with Elastic Cationic Liposomes in Mice Hattori, Yoshiyuki Date, Masataka Arai, Shohei Kawano, Kumi Yonemochi, Etsuo Maitani, Yoshie J Pharm (Cairo) Research Article We developed elastic cationic liposomal vectors for transdermal siRNA delivery. These liposomes were prepared with 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) as a cationic lipid and sodium cholate (NaChol) or Tween 80 as an edge activator. When NaChol or Tween 80 was included at 5, 10, and 15% (w/w) into DOTAP liposomal formulations (C5-, C10-, and C15-liposomes and T5-, T10-, and T15-liposomes), C15- and T10-liposomes showed 2.4- and 2.7-fold-higher elasticities than DOTAP liposome, respectively. Although the sizes of all elastic liposomes prepared in this study were about 80–90 nm, the sizes of C5-, C10- and C15-liposome/siRNA complexes (lipoplexes) were about 1,700–1,800 nm, and those of T5-, T10-, and T15-lipoplexes were about 550–780 nm. Their elastic lipoplexes showed strong gene suppression by siRNA without cytotoxicity when transfected into human cervical carcinoma SiHa cells. Following skin application of the fluorescence-labeled lipoplexes in mice, among the elastic lipoplexes, C15- and T5-lipoplexes showed effective penetration of siRNA into skin, compared with DOTAP lipoplex and free siRNA solution. These data suggest that elastic cationic liposomes containing an appropriate amount of NaChol or Tween 80 as an edge activator could deliver siRNA transdermally. Hindawi Publishing Corporation 2013 2013-12-26 /pmc/articles/PMC4590792/ /pubmed/26555966 http://dx.doi.org/10.1155/2013/149695 Text en Copyright © 2013 Yoshiyuki Hattori et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Hattori, Yoshiyuki
Date, Masataka
Arai, Shohei
Kawano, Kumi
Yonemochi, Etsuo
Maitani, Yoshie
Transdermal Delivery of Small Interfering RNA with Elastic Cationic Liposomes in Mice
title Transdermal Delivery of Small Interfering RNA with Elastic Cationic Liposomes in Mice
title_full Transdermal Delivery of Small Interfering RNA with Elastic Cationic Liposomes in Mice
title_fullStr Transdermal Delivery of Small Interfering RNA with Elastic Cationic Liposomes in Mice
title_full_unstemmed Transdermal Delivery of Small Interfering RNA with Elastic Cationic Liposomes in Mice
title_short Transdermal Delivery of Small Interfering RNA with Elastic Cationic Liposomes in Mice
title_sort transdermal delivery of small interfering rna with elastic cationic liposomes in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4590792/
https://www.ncbi.nlm.nih.gov/pubmed/26555966
http://dx.doi.org/10.1155/2013/149695
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