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Quantum Dot-Loaded Liposomes to Evaluate the Behavior of Drug Carriers after Oral Administration

We have developed submicron-sized liposomes modified with a mucoadhesive polymer to enhance peptide drug absorption after oral administration. Liposomal behavior in the gastrointestinal tract is a critical factor for effective peptide drug delivery. The purpose of this study was to prepare quantum d...

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Autores principales: Tahara, Kohei, Fujimoto, Shiho, Fujii, Fumihiko, Tozuka, Yuichi, Jin, Takashi, Takeuchi, Hirofumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4590795/
https://www.ncbi.nlm.nih.gov/pubmed/26555997
http://dx.doi.org/10.1155/2013/848275
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author Tahara, Kohei
Fujimoto, Shiho
Fujii, Fumihiko
Tozuka, Yuichi
Jin, Takashi
Takeuchi, Hirofumi
author_facet Tahara, Kohei
Fujimoto, Shiho
Fujii, Fumihiko
Tozuka, Yuichi
Jin, Takashi
Takeuchi, Hirofumi
author_sort Tahara, Kohei
collection PubMed
description We have developed submicron-sized liposomes modified with a mucoadhesive polymer to enhance peptide drug absorption after oral administration. Liposomal behavior in the gastrointestinal tract is a critical factor for effective peptide drug delivery. The purpose of this study was to prepare quantum dot- (QD-) loaded submicron-sized liposomes and examine liposomal behavior in the body after oral administration using in vivo fluorescence imaging. Two types of CdSe/CdZnS QDs with different surface properties were used: hydrophobic (unmodified) QDs and hydrophilic QDs with glutathione (GSH) surface modifications. QD- and GSH-QD-loaded liposomes were prepared by a thin film hydration method. Transmission electron microscopy revealed that QDs were embedded in the liposomal lipid bilayer. Conversely, GSH-QDs were present in the inner aqueous phase. Some of the GSH-QDs were electrostatically associated with the lipid membrane of stearylamine-bearing cationic liposomes. QD-loaded liposomes were detected in Caco-2 cells after exposure to the liposomes, and these liposomes were not toxic to the Caco-2 cells. Furthermore, we evaluated the in vivo bioadhesion and intestinal penetration of orally administered QD-loaded liposomes by observing the intestinal segment using confocal laser scanning microscopy.
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spelling pubmed-45907952015-10-13 Quantum Dot-Loaded Liposomes to Evaluate the Behavior of Drug Carriers after Oral Administration Tahara, Kohei Fujimoto, Shiho Fujii, Fumihiko Tozuka, Yuichi Jin, Takashi Takeuchi, Hirofumi J Pharm (Cairo) Research Article We have developed submicron-sized liposomes modified with a mucoadhesive polymer to enhance peptide drug absorption after oral administration. Liposomal behavior in the gastrointestinal tract is a critical factor for effective peptide drug delivery. The purpose of this study was to prepare quantum dot- (QD-) loaded submicron-sized liposomes and examine liposomal behavior in the body after oral administration using in vivo fluorescence imaging. Two types of CdSe/CdZnS QDs with different surface properties were used: hydrophobic (unmodified) QDs and hydrophilic QDs with glutathione (GSH) surface modifications. QD- and GSH-QD-loaded liposomes were prepared by a thin film hydration method. Transmission electron microscopy revealed that QDs were embedded in the liposomal lipid bilayer. Conversely, GSH-QDs were present in the inner aqueous phase. Some of the GSH-QDs were electrostatically associated with the lipid membrane of stearylamine-bearing cationic liposomes. QD-loaded liposomes were detected in Caco-2 cells after exposure to the liposomes, and these liposomes were not toxic to the Caco-2 cells. Furthermore, we evaluated the in vivo bioadhesion and intestinal penetration of orally administered QD-loaded liposomes by observing the intestinal segment using confocal laser scanning microscopy. Hindawi Publishing Corporation 2013 2013-07-18 /pmc/articles/PMC4590795/ /pubmed/26555997 http://dx.doi.org/10.1155/2013/848275 Text en Copyright © 2013 Kohei Tahara et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Tahara, Kohei
Fujimoto, Shiho
Fujii, Fumihiko
Tozuka, Yuichi
Jin, Takashi
Takeuchi, Hirofumi
Quantum Dot-Loaded Liposomes to Evaluate the Behavior of Drug Carriers after Oral Administration
title Quantum Dot-Loaded Liposomes to Evaluate the Behavior of Drug Carriers after Oral Administration
title_full Quantum Dot-Loaded Liposomes to Evaluate the Behavior of Drug Carriers after Oral Administration
title_fullStr Quantum Dot-Loaded Liposomes to Evaluate the Behavior of Drug Carriers after Oral Administration
title_full_unstemmed Quantum Dot-Loaded Liposomes to Evaluate the Behavior of Drug Carriers after Oral Administration
title_short Quantum Dot-Loaded Liposomes to Evaluate the Behavior of Drug Carriers after Oral Administration
title_sort quantum dot-loaded liposomes to evaluate the behavior of drug carriers after oral administration
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4590795/
https://www.ncbi.nlm.nih.gov/pubmed/26555997
http://dx.doi.org/10.1155/2013/848275
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