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Quantum Dot-Loaded Liposomes to Evaluate the Behavior of Drug Carriers after Oral Administration
We have developed submicron-sized liposomes modified with a mucoadhesive polymer to enhance peptide drug absorption after oral administration. Liposomal behavior in the gastrointestinal tract is a critical factor for effective peptide drug delivery. The purpose of this study was to prepare quantum d...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4590795/ https://www.ncbi.nlm.nih.gov/pubmed/26555997 http://dx.doi.org/10.1155/2013/848275 |
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author | Tahara, Kohei Fujimoto, Shiho Fujii, Fumihiko Tozuka, Yuichi Jin, Takashi Takeuchi, Hirofumi |
author_facet | Tahara, Kohei Fujimoto, Shiho Fujii, Fumihiko Tozuka, Yuichi Jin, Takashi Takeuchi, Hirofumi |
author_sort | Tahara, Kohei |
collection | PubMed |
description | We have developed submicron-sized liposomes modified with a mucoadhesive polymer to enhance peptide drug absorption after oral administration. Liposomal behavior in the gastrointestinal tract is a critical factor for effective peptide drug delivery. The purpose of this study was to prepare quantum dot- (QD-) loaded submicron-sized liposomes and examine liposomal behavior in the body after oral administration using in vivo fluorescence imaging. Two types of CdSe/CdZnS QDs with different surface properties were used: hydrophobic (unmodified) QDs and hydrophilic QDs with glutathione (GSH) surface modifications. QD- and GSH-QD-loaded liposomes were prepared by a thin film hydration method. Transmission electron microscopy revealed that QDs were embedded in the liposomal lipid bilayer. Conversely, GSH-QDs were present in the inner aqueous phase. Some of the GSH-QDs were electrostatically associated with the lipid membrane of stearylamine-bearing cationic liposomes. QD-loaded liposomes were detected in Caco-2 cells after exposure to the liposomes, and these liposomes were not toxic to the Caco-2 cells. Furthermore, we evaluated the in vivo bioadhesion and intestinal penetration of orally administered QD-loaded liposomes by observing the intestinal segment using confocal laser scanning microscopy. |
format | Online Article Text |
id | pubmed-4590795 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-45907952015-10-13 Quantum Dot-Loaded Liposomes to Evaluate the Behavior of Drug Carriers after Oral Administration Tahara, Kohei Fujimoto, Shiho Fujii, Fumihiko Tozuka, Yuichi Jin, Takashi Takeuchi, Hirofumi J Pharm (Cairo) Research Article We have developed submicron-sized liposomes modified with a mucoadhesive polymer to enhance peptide drug absorption after oral administration. Liposomal behavior in the gastrointestinal tract is a critical factor for effective peptide drug delivery. The purpose of this study was to prepare quantum dot- (QD-) loaded submicron-sized liposomes and examine liposomal behavior in the body after oral administration using in vivo fluorescence imaging. Two types of CdSe/CdZnS QDs with different surface properties were used: hydrophobic (unmodified) QDs and hydrophilic QDs with glutathione (GSH) surface modifications. QD- and GSH-QD-loaded liposomes were prepared by a thin film hydration method. Transmission electron microscopy revealed that QDs were embedded in the liposomal lipid bilayer. Conversely, GSH-QDs were present in the inner aqueous phase. Some of the GSH-QDs were electrostatically associated with the lipid membrane of stearylamine-bearing cationic liposomes. QD-loaded liposomes were detected in Caco-2 cells after exposure to the liposomes, and these liposomes were not toxic to the Caco-2 cells. Furthermore, we evaluated the in vivo bioadhesion and intestinal penetration of orally administered QD-loaded liposomes by observing the intestinal segment using confocal laser scanning microscopy. Hindawi Publishing Corporation 2013 2013-07-18 /pmc/articles/PMC4590795/ /pubmed/26555997 http://dx.doi.org/10.1155/2013/848275 Text en Copyright © 2013 Kohei Tahara et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Tahara, Kohei Fujimoto, Shiho Fujii, Fumihiko Tozuka, Yuichi Jin, Takashi Takeuchi, Hirofumi Quantum Dot-Loaded Liposomes to Evaluate the Behavior of Drug Carriers after Oral Administration |
title | Quantum Dot-Loaded Liposomes to Evaluate the Behavior of Drug Carriers after Oral Administration |
title_full | Quantum Dot-Loaded Liposomes to Evaluate the Behavior of Drug Carriers after Oral Administration |
title_fullStr | Quantum Dot-Loaded Liposomes to Evaluate the Behavior of Drug Carriers after Oral Administration |
title_full_unstemmed | Quantum Dot-Loaded Liposomes to Evaluate the Behavior of Drug Carriers after Oral Administration |
title_short | Quantum Dot-Loaded Liposomes to Evaluate the Behavior of Drug Carriers after Oral Administration |
title_sort | quantum dot-loaded liposomes to evaluate the behavior of drug carriers after oral administration |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4590795/ https://www.ncbi.nlm.nih.gov/pubmed/26555997 http://dx.doi.org/10.1155/2013/848275 |
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