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Activation of Natural Killer Cells in Patients with Chronic Bone and Joint Infection due to Staphylococci Expressing or Not the Small Colony Variant Phenotype

Chronic bone and joint infections (BJI) are devastating diseases. Relapses are frequently observed, as some pathogens, especially staphylococci, can persist intracellularly by expressing a particular phenotype called small colony variant (SCV). As natural killer (NK) cells are lymphocytes specialize...

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Autores principales: Viel, Sébastien, Rouzaire, Paul, Laurent, Frédéric, Walzer, Thierry, Bienvenu, Jacques, Valour, Florent, Chidiac, Christian, Ferry, Tristan, Group, The Lyon BJI Study
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4590914/
https://www.ncbi.nlm.nih.gov/pubmed/26464851
http://dx.doi.org/10.1155/2014/280653
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author Viel, Sébastien
Rouzaire, Paul
Laurent, Frédéric
Walzer, Thierry
Bienvenu, Jacques
Valour, Florent
Chidiac, Christian
Ferry, Tristan
Group, The Lyon BJI Study
author_facet Viel, Sébastien
Rouzaire, Paul
Laurent, Frédéric
Walzer, Thierry
Bienvenu, Jacques
Valour, Florent
Chidiac, Christian
Ferry, Tristan
Group, The Lyon BJI Study
author_sort Viel, Sébastien
collection PubMed
description Chronic bone and joint infections (BJI) are devastating diseases. Relapses are frequently observed, as some pathogens, especially staphylococci, can persist intracellularly by expressing a particular phenotype called small colony variant (SCV). As natural killer (NK) cells are lymphocytes specialized in the killing of host cells infected by intracellular pathogens, we studied NK cells of patients with chronic BJI due to staphylococci expressing or not SCVs (10 patients in both groups). Controls were patients infected with other bacteria without detectable expression of SCVs, and healthy volunteers. NK cell phenotype was evaluated from PBMCs by flow cytometry. Degranulation capacity was evaluated after stimulation with K562 cells in vitro. We found that NK cells were activated in terms of CD69 expression, loss of CD16 and perforin, in all infected patients in comparison with healthy volunteers, independently of the SCV phenotype. Peripheral NK cells in patients with chronic BJI display signs of recent activation and degranulation in vivo in response to CD16-mediated signals, regardless of the type of bacteria involved. This could involve a universal capacity of isolates responsible for chronic BJI to produce undetectable SCVs in vivo, which might be a target of future intervention.
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spelling pubmed-45909142015-10-13 Activation of Natural Killer Cells in Patients with Chronic Bone and Joint Infection due to Staphylococci Expressing or Not the Small Colony Variant Phenotype Viel, Sébastien Rouzaire, Paul Laurent, Frédéric Walzer, Thierry Bienvenu, Jacques Valour, Florent Chidiac, Christian Ferry, Tristan Group, The Lyon BJI Study Int J Chronic Dis Clinical Study Chronic bone and joint infections (BJI) are devastating diseases. Relapses are frequently observed, as some pathogens, especially staphylococci, can persist intracellularly by expressing a particular phenotype called small colony variant (SCV). As natural killer (NK) cells are lymphocytes specialized in the killing of host cells infected by intracellular pathogens, we studied NK cells of patients with chronic BJI due to staphylococci expressing or not SCVs (10 patients in both groups). Controls were patients infected with other bacteria without detectable expression of SCVs, and healthy volunteers. NK cell phenotype was evaluated from PBMCs by flow cytometry. Degranulation capacity was evaluated after stimulation with K562 cells in vitro. We found that NK cells were activated in terms of CD69 expression, loss of CD16 and perforin, in all infected patients in comparison with healthy volunteers, independently of the SCV phenotype. Peripheral NK cells in patients with chronic BJI display signs of recent activation and degranulation in vivo in response to CD16-mediated signals, regardless of the type of bacteria involved. This could involve a universal capacity of isolates responsible for chronic BJI to produce undetectable SCVs in vivo, which might be a target of future intervention. Hindawi Publishing Corporation 2014 2014-03-03 /pmc/articles/PMC4590914/ /pubmed/26464851 http://dx.doi.org/10.1155/2014/280653 Text en Copyright © 2014 Sébastien Viel et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Clinical Study
Viel, Sébastien
Rouzaire, Paul
Laurent, Frédéric
Walzer, Thierry
Bienvenu, Jacques
Valour, Florent
Chidiac, Christian
Ferry, Tristan
Group, The Lyon BJI Study
Activation of Natural Killer Cells in Patients with Chronic Bone and Joint Infection due to Staphylococci Expressing or Not the Small Colony Variant Phenotype
title Activation of Natural Killer Cells in Patients with Chronic Bone and Joint Infection due to Staphylococci Expressing or Not the Small Colony Variant Phenotype
title_full Activation of Natural Killer Cells in Patients with Chronic Bone and Joint Infection due to Staphylococci Expressing or Not the Small Colony Variant Phenotype
title_fullStr Activation of Natural Killer Cells in Patients with Chronic Bone and Joint Infection due to Staphylococci Expressing or Not the Small Colony Variant Phenotype
title_full_unstemmed Activation of Natural Killer Cells in Patients with Chronic Bone and Joint Infection due to Staphylococci Expressing or Not the Small Colony Variant Phenotype
title_short Activation of Natural Killer Cells in Patients with Chronic Bone and Joint Infection due to Staphylococci Expressing or Not the Small Colony Variant Phenotype
title_sort activation of natural killer cells in patients with chronic bone and joint infection due to staphylococci expressing or not the small colony variant phenotype
topic Clinical Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4590914/
https://www.ncbi.nlm.nih.gov/pubmed/26464851
http://dx.doi.org/10.1155/2014/280653
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