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Small RNA and Degradome Sequencing Reveal Complex Roles of miRNAs and Their Targets in Developing Wheat Grains

Plant microRNAs (miRNAs) have been shown to play critical roles in plant development. In this study, we employed small RNA combined with degradome sequencing to survey development-related miRNAs and their validated targets during wheat grain development. A total of 186 known miRNAs and 37 novel miRN...

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Autores principales: Li, Tian, Ma, Lin, Geng, Yuke, Hao, Chenyang, Chen, Xinhong, Zhang, Xueyong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4591353/
https://www.ncbi.nlm.nih.gov/pubmed/26426440
http://dx.doi.org/10.1371/journal.pone.0139658
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author Li, Tian
Ma, Lin
Geng, Yuke
Hao, Chenyang
Chen, Xinhong
Zhang, Xueyong
author_facet Li, Tian
Ma, Lin
Geng, Yuke
Hao, Chenyang
Chen, Xinhong
Zhang, Xueyong
author_sort Li, Tian
collection PubMed
description Plant microRNAs (miRNAs) have been shown to play critical roles in plant development. In this study, we employed small RNA combined with degradome sequencing to survey development-related miRNAs and their validated targets during wheat grain development. A total of 186 known miRNAs and 37 novel miRNAs were identified in four small RNA libraries. Moreover, a miRNA-like long hairpin locus was first identified to produce 21~22-nt phased siRNAs that act in trans to cleave target mRNAs. A comparison of the miRNAomes revealed that 55 miRNA families were differentially expressed during the grain development. Predicted and validated targets of these development-related miRNAs are involved in different cellular responses and metabolic processes including cell proliferation, auxin signaling, nutrient metabolism and gene expression. This study provides insight into the complex roles of miRNAs and their targets in regulating wheat grain development.
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spelling pubmed-45913532015-10-09 Small RNA and Degradome Sequencing Reveal Complex Roles of miRNAs and Their Targets in Developing Wheat Grains Li, Tian Ma, Lin Geng, Yuke Hao, Chenyang Chen, Xinhong Zhang, Xueyong PLoS One Research Article Plant microRNAs (miRNAs) have been shown to play critical roles in plant development. In this study, we employed small RNA combined with degradome sequencing to survey development-related miRNAs and their validated targets during wheat grain development. A total of 186 known miRNAs and 37 novel miRNAs were identified in four small RNA libraries. Moreover, a miRNA-like long hairpin locus was first identified to produce 21~22-nt phased siRNAs that act in trans to cleave target mRNAs. A comparison of the miRNAomes revealed that 55 miRNA families were differentially expressed during the grain development. Predicted and validated targets of these development-related miRNAs are involved in different cellular responses and metabolic processes including cell proliferation, auxin signaling, nutrient metabolism and gene expression. This study provides insight into the complex roles of miRNAs and their targets in regulating wheat grain development. Public Library of Science 2015-10-01 /pmc/articles/PMC4591353/ /pubmed/26426440 http://dx.doi.org/10.1371/journal.pone.0139658 Text en © 2015 Li et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Li, Tian
Ma, Lin
Geng, Yuke
Hao, Chenyang
Chen, Xinhong
Zhang, Xueyong
Small RNA and Degradome Sequencing Reveal Complex Roles of miRNAs and Their Targets in Developing Wheat Grains
title Small RNA and Degradome Sequencing Reveal Complex Roles of miRNAs and Their Targets in Developing Wheat Grains
title_full Small RNA and Degradome Sequencing Reveal Complex Roles of miRNAs and Their Targets in Developing Wheat Grains
title_fullStr Small RNA and Degradome Sequencing Reveal Complex Roles of miRNAs and Their Targets in Developing Wheat Grains
title_full_unstemmed Small RNA and Degradome Sequencing Reveal Complex Roles of miRNAs and Their Targets in Developing Wheat Grains
title_short Small RNA and Degradome Sequencing Reveal Complex Roles of miRNAs and Their Targets in Developing Wheat Grains
title_sort small rna and degradome sequencing reveal complex roles of mirnas and their targets in developing wheat grains
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4591353/
https://www.ncbi.nlm.nih.gov/pubmed/26426440
http://dx.doi.org/10.1371/journal.pone.0139658
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