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Detection of N-(1-deoxy-d-fructos-1-yl) Fumonisins B(2) and B(3) in Corn by High-Resolution LC-Orbitrap MS
The existence of glucose conjugates of fumonisin B(2) (FB(2)) and fumonisin B(3) (FB(3)) in corn powder was confirmed for the first time. These “bound-fumonisins” (FB(2) and FB(3) bound to glucose) were identified as N-(1-deoxy-d-fructos-1-yl) fumonisin B(2) (NDfrc-FB(2)) and N-(1-deoxy-d-fructos-1-...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4591641/ https://www.ncbi.nlm.nih.gov/pubmed/26389955 http://dx.doi.org/10.3390/toxins7093700 |
Sumario: | The existence of glucose conjugates of fumonisin B(2) (FB(2)) and fumonisin B(3) (FB(3)) in corn powder was confirmed for the first time. These “bound-fumonisins” (FB(2) and FB(3) bound to glucose) were identified as N-(1-deoxy-d-fructos-1-yl) fumonisin B(2) (NDfrc-FB(2)) and N-(1-deoxy-d-fructos-1-yl) fumonisin B(3) (NDfrc-FB(3)) respectively, based on the accurate mass measurements of characteristic ions and fragmentation patterns using high-resolution liquid chromatography-Orbitrap mass spectrometry (LC-Orbitrap MS) analysis. Treatment on NDfrc-FB(2) and NDfrc-FB(3) with the o-phthalaldehyde (OPA) reagent also supported that d-glucose binding to FB(2) and FB(3) molecules occurred to their primary amine residues. |
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